Job ID = 11633832 sra ファイルのダウンロード中... Completed: 110246K bytes transferred in 5 seconds (152195K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 3424330 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420449/ERR1348925.sra Written 3424330 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420449/ERR1348925.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:37 3424330 reads; of these: 3424330 (100.00%) were unpaired; of these: 339855 (9.92%) aligned 0 times 2665659 (77.84%) aligned exactly 1 time 418816 (12.23%) aligned >1 times 90.08% overall alignment rate Time searching: 00:00:37 Overall time: 00:00:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 725382 / 3084475 = 0.2352 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:18:30: # Command line: callpeak -t ERX1420449.bam -f BAM -g 12100000 -n ERX1420449.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1420449.20 # format = BAM # ChIP-seq file = ['ERX1420449.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:18:30: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:18:30: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:18:30: # Command line: callpeak -t ERX1420449.bam -f BAM -g 12100000 -n ERX1420449.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1420449.10 # format = BAM # ChIP-seq file = ['ERX1420449.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:18:30: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:18:30: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:18:30: # Command line: callpeak -t ERX1420449.bam -f BAM -g 12100000 -n ERX1420449.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1420449.05 # format = BAM # ChIP-seq file = ['ERX1420449.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:18:30: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:18:30: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:18:37: 1000000 INFO @ Fri, 15 Feb 2019 09:18:38: 1000000 INFO @ Fri, 15 Feb 2019 09:18:38: 1000000 INFO @ Fri, 15 Feb 2019 09:18:45: 2000000 INFO @ Fri, 15 Feb 2019 09:18:45: 2000000 INFO @ Fri, 15 Feb 2019 09:18:46: 2000000 INFO @ Fri, 15 Feb 2019 09:18:47: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:18:47: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:18:47: #1 total tags in treatment: 2359093 INFO @ Fri, 15 Feb 2019 09:18:47: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:18:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:18:47: #1 tags after filtering in treatment: 2359093 INFO @ Fri, 15 Feb 2019 09:18:47: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:18:47: #1 finished! INFO @ Fri, 15 Feb 2019 09:18:47: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:18:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:18:47: #2 number of paired peaks: 177 WARNING @ Fri, 15 Feb 2019 09:18:47: Fewer paired peaks (177) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 177 pairs to build model! INFO @ Fri, 15 Feb 2019 09:18:47: start model_add_line... INFO @ Fri, 15 Feb 2019 09:18:47: start X-correlation... INFO @ Fri, 15 Feb 2019 09:18:47: end of X-cor INFO @ Fri, 15 Feb 2019 09:18:47: #2 finished! INFO @ Fri, 15 Feb 2019 09:18:47: #2 predicted fragment length is 303 bps INFO @ Fri, 15 Feb 2019 09:18:47: #2 alternative fragment length(s) may be 170,195,232,259,303,332 bps INFO @ Fri, 15 Feb 2019 09:18:47: #2.2 Generate R script for model : ERX1420449.10_model.r INFO @ Fri, 15 Feb 2019 09:18:47: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:18:47: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:18:48: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:18:48: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:18:48: #1 total tags in treatment: 2359093 INFO @ Fri, 15 Feb 2019 09:18:48: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:18:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:18:48: #1 tags after filtering in treatment: 2359093 INFO @ Fri, 15 Feb 2019 09:18:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:18:48: #1 finished! INFO @ Fri, 15 Feb 2019 09:18:48: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:18:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:18:48: #2 number of paired peaks: 177 WARNING @ Fri, 15 Feb 2019 09:18:48: Fewer paired peaks (177) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 177 pairs to build model! INFO @ Fri, 15 Feb 2019 09:18:48: start model_add_line... INFO @ Fri, 15 Feb 2019 09:18:48: start X-correlation... INFO @ Fri, 15 Feb 2019 09:18:48: end of X-cor INFO @ Fri, 15 Feb 2019 09:18:48: #2 finished! INFO @ Fri, 15 Feb 2019 09:18:48: #2 predicted fragment length is 303 bps INFO @ Fri, 15 Feb 2019 09:18:48: #2 alternative fragment length(s) may be 170,195,232,259,303,332 bps INFO @ Fri, 15 Feb 2019 09:18:48: #2.2 Generate R script for model : ERX1420449.20_model.r INFO @ Fri, 15 Feb 2019 09:18:48: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:18:48: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:18:48: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:18:48: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:18:48: #1 total tags in treatment: 2359093 INFO @ Fri, 15 Feb 2019 09:18:48: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:18:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:18:48: #1 tags after filtering in treatment: 2359093 INFO @ Fri, 15 Feb 2019 09:18:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:18:48: #1 finished! INFO @ Fri, 15 Feb 2019 09:18:48: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:18:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:18:48: #2 number of paired peaks: 177 WARNING @ Fri, 15 Feb 2019 09:18:48: Fewer paired peaks (177) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 177 pairs to build model! INFO @ Fri, 15 Feb 2019 09:18:48: start model_add_line... INFO @ Fri, 15 Feb 2019 09:18:49: start X-correlation... INFO @ Fri, 15 Feb 2019 09:18:49: end of X-cor INFO @ Fri, 15 Feb 2019 09:18:49: #2 finished! INFO @ Fri, 15 Feb 2019 09:18:49: #2 predicted fragment length is 303 bps INFO @ Fri, 15 Feb 2019 09:18:49: #2 alternative fragment length(s) may be 170,195,232,259,303,332 bps INFO @ Fri, 15 Feb 2019 09:18:49: #2.2 Generate R script for model : ERX1420449.05_model.r INFO @ Fri, 15 Feb 2019 09:18:49: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:18:49: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:18:54: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:18:54: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:18:55: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:18:56: #4 Write output xls file... ERX1420449.10_peaks.xls INFO @ Fri, 15 Feb 2019 09:18:56: #4 Write peak in narrowPeak format file... ERX1420449.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:18:56: #4 Write summits bed file... ERX1420449.10_summits.bed INFO @ Fri, 15 Feb 2019 09:18:56: Done! pass1 - making usageList (5 chroms): 4 millis pass2 - checking and writing primary data (9 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:18:57: #4 Write output xls file... ERX1420449.20_peaks.xls INFO @ Fri, 15 Feb 2019 09:18:57: #4 Write peak in narrowPeak format file... ERX1420449.20_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:18:57: #4 Write summits bed file... ERX1420449.20_summits.bed INFO @ Fri, 15 Feb 2019 09:18:57: Done! pass1 - making usageList (5 chroms): 3 millis pass2 - checking and writing primary data (9 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:18:57: #4 Write output xls file... ERX1420449.05_peaks.xls INFO @ Fri, 15 Feb 2019 09:18:57: #4 Write peak in narrowPeak format file... ERX1420449.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:18:57: #4 Write summits bed file... ERX1420449.05_summits.bed INFO @ Fri, 15 Feb 2019 09:18:57: Done! pass1 - making usageList (8 chroms): 3 millis pass2 - checking and writing primary data (14 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。