Job ID = 11633725 sra ファイルのダウンロード中... Completed: 31479K bytes transferred in 3 seconds (79661K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 973462 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420344/ERR1348820.sra Written 973462 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420344/ERR1348820.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:08 973462 reads; of these: 973462 (100.00%) were unpaired; of these: 429496 (44.12%) aligned 0 times 471481 (48.43%) aligned exactly 1 time 72485 (7.45%) aligned >1 times 55.88% overall alignment rate Time searching: 00:00:08 Overall time: 00:00:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 24898 / 543966 = 0.0458 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:10:26: # Command line: callpeak -t ERX1420344.bam -f BAM -g 12100000 -n ERX1420344.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1420344.10 # format = BAM # ChIP-seq file = ['ERX1420344.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:10:26: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:10:26: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:10:26: # Command line: callpeak -t ERX1420344.bam -f BAM -g 12100000 -n ERX1420344.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1420344.05 # format = BAM # ChIP-seq file = ['ERX1420344.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:10:26: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:10:26: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:10:26: # Command line: callpeak -t ERX1420344.bam -f BAM -g 12100000 -n ERX1420344.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1420344.20 # format = BAM # ChIP-seq file = ['ERX1420344.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:10:26: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:10:26: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:10:30: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:10:30: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:10:30: #1 total tags in treatment: 519068 INFO @ Fri, 15 Feb 2019 09:10:30: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:10:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:10:30: #1 tags after filtering in treatment: 519068 INFO @ Fri, 15 Feb 2019 09:10:30: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:10:30: #1 finished! INFO @ Fri, 15 Feb 2019 09:10:30: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:10:30: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:10:30: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:10:30: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:10:30: #1 total tags in treatment: 519068 INFO @ Fri, 15 Feb 2019 09:10:30: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:10:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:10:30: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:10:30: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:10:30: #1 total tags in treatment: 519068 INFO @ Fri, 15 Feb 2019 09:10:30: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:10:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:10:30: #1 tags after filtering in treatment: 519068 INFO @ Fri, 15 Feb 2019 09:10:30: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:10:30: #1 finished! INFO @ Fri, 15 Feb 2019 09:10:30: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:10:30: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:10:30: #1 tags after filtering in treatment: 519068 INFO @ Fri, 15 Feb 2019 09:10:30: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:10:30: #1 finished! INFO @ Fri, 15 Feb 2019 09:10:30: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:10:30: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:10:30: #2 number of paired peaks: 35 WARNING @ Fri, 15 Feb 2019 09:10:30: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:10:30: Process for pairing-model is terminated! INFO @ Fri, 15 Feb 2019 09:10:30: #2 number of paired peaks: 35 WARNING @ Fri, 15 Feb 2019 09:10:30: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:10:30: Process for pairing-model is terminated! cat: ERX1420344.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: ERX1420344.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Fri, 15 Feb 2019 09:10:30: #2 number of paired peaks: 35 WARNING @ Fri, 15 Feb 2019 09:10:30: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:10:30: Process for pairing-model is terminated! pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) cat: ERX1420344.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 4 millis rm: cannot remove `ERX1420344.10_model.r': そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1420344.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420344.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling rm: cannot remove `ERX1420344.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420344.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420344.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling rm: cannot remove `ERX1420344.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420344.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420344.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。