Job ID = 11633660 sra ファイルのダウンロード中... Completed: 99602K bytes transferred in 4 seconds (174889K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 3081791 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420279/ERR1348755.sra Written 3081791 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420279/ERR1348755.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:31 3081791 reads; of these: 3081791 (100.00%) were unpaired; of these: 451097 (14.64%) aligned 0 times 2159342 (70.07%) aligned exactly 1 time 471352 (15.29%) aligned >1 times 85.36% overall alignment rate Time searching: 00:00:31 Overall time: 00:00:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 515928 / 2630694 = 0.1961 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:08:28: # Command line: callpeak -t ERX1420279.bam -f BAM -g 12100000 -n ERX1420279.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1420279.05 # format = BAM # ChIP-seq file = ['ERX1420279.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:08:28: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:08:28: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:08:28: # Command line: callpeak -t ERX1420279.bam -f BAM -g 12100000 -n ERX1420279.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1420279.10 # format = BAM # ChIP-seq file = ['ERX1420279.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:08:28: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:08:28: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:08:28: # Command line: callpeak -t ERX1420279.bam -f BAM -g 12100000 -n ERX1420279.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1420279.20 # format = BAM # ChIP-seq file = ['ERX1420279.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:08:28: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:08:28: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:08:34: 1000000 INFO @ Fri, 15 Feb 2019 09:08:34: 1000000 INFO @ Fri, 15 Feb 2019 09:08:34: 1000000 INFO @ Fri, 15 Feb 2019 09:08:40: 2000000 INFO @ Fri, 15 Feb 2019 09:08:41: 2000000 INFO @ Fri, 15 Feb 2019 09:08:41: 2000000 INFO @ Fri, 15 Feb 2019 09:08:41: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:08:41: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:08:41: #1 total tags in treatment: 2114766 INFO @ Fri, 15 Feb 2019 09:08:41: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:08:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:08:41: #1 tags after filtering in treatment: 2114766 INFO @ Fri, 15 Feb 2019 09:08:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:08:41: #1 finished! INFO @ Fri, 15 Feb 2019 09:08:41: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:08:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:08:41: #2 number of paired peaks: 92 WARNING @ Fri, 15 Feb 2019 09:08:41: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:08:41: Process for pairing-model is terminated! cat: ERX1420279.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 9 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1420279.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420279.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420279.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:08:42: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:08:42: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:08:42: #1 total tags in treatment: 2114766 INFO @ Fri, 15 Feb 2019 09:08:42: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:08:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:08:42: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:08:42: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:08:42: #1 total tags in treatment: 2114766 INFO @ Fri, 15 Feb 2019 09:08:42: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:08:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:08:42: #1 tags after filtering in treatment: 2114766 INFO @ Fri, 15 Feb 2019 09:08:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:08:42: #1 finished! INFO @ Fri, 15 Feb 2019 09:08:42: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:08:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:08:42: #1 tags after filtering in treatment: 2114766 INFO @ Fri, 15 Feb 2019 09:08:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:08:42: #1 finished! INFO @ Fri, 15 Feb 2019 09:08:42: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:08:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:08:42: #2 number of paired peaks: 92 WARNING @ Fri, 15 Feb 2019 09:08:42: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:08:42: Process for pairing-model is terminated! INFO @ Fri, 15 Feb 2019 09:08:42: #2 number of paired peaks: 92 WARNING @ Fri, 15 Feb 2019 09:08:42: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:08:42: Process for pairing-model is terminated! cat: ERX1420279.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: ERX1420279.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 11 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1420279.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420279.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420279.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 11 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling rm: cannot remove `ERX1420279.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420279.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420279.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。