Job ID = 11633655 sra ファイルのダウンロード中... Completed: 130531K bytes transferred in 5 seconds (196535K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420274/ERR1348750.sra Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420274/ERR1348750.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:38 4000000 reads; of these: 4000000 (100.00%) were unpaired; of these: 671786 (16.79%) aligned 0 times 2989493 (74.74%) aligned exactly 1 time 338721 (8.47%) aligned >1 times 83.21% overall alignment rate Time searching: 00:00:38 Overall time: 00:00:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 396700 / 3328214 = 0.1192 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:08:36: # Command line: callpeak -t ERX1420274.bam -f BAM -g 12100000 -n ERX1420274.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1420274.05 # format = BAM # ChIP-seq file = ['ERX1420274.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:08:36: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:08:36: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:08:36: # Command line: callpeak -t ERX1420274.bam -f BAM -g 12100000 -n ERX1420274.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1420274.10 # format = BAM # ChIP-seq file = ['ERX1420274.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:08:36: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:08:36: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:08:36: # Command line: callpeak -t ERX1420274.bam -f BAM -g 12100000 -n ERX1420274.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1420274.20 # format = BAM # ChIP-seq file = ['ERX1420274.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:08:36: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:08:36: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:08:42: 1000000 INFO @ Fri, 15 Feb 2019 09:08:42: 1000000 INFO @ Fri, 15 Feb 2019 09:08:42: 1000000 INFO @ Fri, 15 Feb 2019 09:08:48: 2000000 INFO @ Fri, 15 Feb 2019 09:08:48: 2000000 INFO @ Fri, 15 Feb 2019 09:08:49: 2000000 INFO @ Fri, 15 Feb 2019 09:08:54: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:08:54: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:08:54: #1 total tags in treatment: 2931514 INFO @ Fri, 15 Feb 2019 09:08:54: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:08:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:08:54: #1 tags after filtering in treatment: 2931514 INFO @ Fri, 15 Feb 2019 09:08:54: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:08:54: #1 finished! INFO @ Fri, 15 Feb 2019 09:08:54: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:08:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:08:54: #2 number of paired peaks: 29 WARNING @ Fri, 15 Feb 2019 09:08:54: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:08:54: Process for pairing-model is terminated! cat: ERX1420274.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1420274.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420274.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420274.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:08:54: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:08:54: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:08:54: #1 total tags in treatment: 2931514 INFO @ Fri, 15 Feb 2019 09:08:54: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:08:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:08:54: #1 tags after filtering in treatment: 2931514 INFO @ Fri, 15 Feb 2019 09:08:54: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:08:54: #1 finished! INFO @ Fri, 15 Feb 2019 09:08:54: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:08:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:08:55: #2 number of paired peaks: 29 WARNING @ Fri, 15 Feb 2019 09:08:55: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:08:55: Process for pairing-model is terminated! cat: ERX1420274.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1420274.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420274.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420274.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:08:55: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:08:55: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:08:55: #1 total tags in treatment: 2931514 INFO @ Fri, 15 Feb 2019 09:08:55: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:08:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:08:55: #1 tags after filtering in treatment: 2931514 INFO @ Fri, 15 Feb 2019 09:08:55: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:08:55: #1 finished! INFO @ Fri, 15 Feb 2019 09:08:55: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:08:55: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:08:55: #2 number of paired peaks: 29 WARNING @ Fri, 15 Feb 2019 09:08:55: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:08:55: Process for pairing-model is terminated! cat: ERX1420274.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1420274.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420274.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420274.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。