Job ID = 11633616 sra ファイルのダウンロード中... Completed: 127934K bytes transferred in 5 seconds (202223K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 1756900 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420235/ERR1348711.sra Written 1756900 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420235/ERR1348711.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:54 1756900 reads; of these: 1756900 (100.00%) were paired; of these: 1457262 (82.95%) aligned concordantly 0 times 251497 (14.31%) aligned concordantly exactly 1 time 48141 (2.74%) aligned concordantly >1 times ---- 1457262 pairs aligned concordantly 0 times; of these: 261855 (17.97%) aligned discordantly 1 time ---- 1195407 pairs aligned 0 times concordantly or discordantly; of these: 2390814 mates make up the pairs; of these: 2076935 (86.87%) aligned 0 times 219017 (9.16%) aligned exactly 1 time 94862 (3.97%) aligned >1 times 40.89% overall alignment rate Time searching: 00:00:54 Overall time: 00:00:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 115744 / 386763 = 0.2993 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:05:54: # Command line: callpeak -t ERX1420235.bam -f BAM -g 12100000 -n ERX1420235.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1420235.10 # format = BAM # ChIP-seq file = ['ERX1420235.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:05:54: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:05:54: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:05:54: # Command line: callpeak -t ERX1420235.bam -f BAM -g 12100000 -n ERX1420235.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1420235.05 # format = BAM # ChIP-seq file = ['ERX1420235.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:05:54: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:05:54: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:05:54: # Command line: callpeak -t ERX1420235.bam -f BAM -g 12100000 -n ERX1420235.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1420235.20 # format = BAM # ChIP-seq file = ['ERX1420235.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:05:54: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:05:54: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:05:59: 1000000 INFO @ Fri, 15 Feb 2019 09:05:59: 1000000 INFO @ Fri, 15 Feb 2019 09:05:59: 1000000 INFO @ Fri, 15 Feb 2019 09:06:00: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:06:00: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:06:00: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:06:00: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:06:00: #1 total tags in treatment: 209195 INFO @ Fri, 15 Feb 2019 09:06:00: #1 total tags in treatment: 209195 INFO @ Fri, 15 Feb 2019 09:06:00: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:06:00: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:06:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:06:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:06:00: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:06:00: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:06:00: #1 total tags in treatment: 209195 INFO @ Fri, 15 Feb 2019 09:06:00: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:06:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:06:00: #1 tags after filtering in treatment: 199120 INFO @ Fri, 15 Feb 2019 09:06:00: #1 Redundant rate of treatment: 0.05 INFO @ Fri, 15 Feb 2019 09:06:00: #1 finished! INFO @ Fri, 15 Feb 2019 09:06:00: #1 tags after filtering in treatment: 199120 INFO @ Fri, 15 Feb 2019 09:06:00: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:06:00: #1 Redundant rate of treatment: 0.05 INFO @ Fri, 15 Feb 2019 09:06:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:06:00: #1 finished! INFO @ Fri, 15 Feb 2019 09:06:00: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:06:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:06:00: #1 tags after filtering in treatment: 199120 INFO @ Fri, 15 Feb 2019 09:06:00: #1 Redundant rate of treatment: 0.05 INFO @ Fri, 15 Feb 2019 09:06:00: #1 finished! INFO @ Fri, 15 Feb 2019 09:06:00: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:06:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:06:01: #2 number of paired peaks: 118 WARNING @ Fri, 15 Feb 2019 09:06:01: Fewer paired peaks (118) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 118 pairs to build model! INFO @ Fri, 15 Feb 2019 09:06:01: start model_add_line... INFO @ Fri, 15 Feb 2019 09:06:01: #2 number of paired peaks: 118 WARNING @ Fri, 15 Feb 2019 09:06:01: Fewer paired peaks (118) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 118 pairs to build model! INFO @ Fri, 15 Feb 2019 09:06:01: start model_add_line... INFO @ Fri, 15 Feb 2019 09:06:01: start X-correlation... INFO @ Fri, 15 Feb 2019 09:06:01: start X-correlation... INFO @ Fri, 15 Feb 2019 09:06:01: #2 number of paired peaks: 118 WARNING @ Fri, 15 Feb 2019 09:06:01: Fewer paired peaks (118) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 118 pairs to build model! INFO @ Fri, 15 Feb 2019 09:06:01: start model_add_line... INFO @ Fri, 15 Feb 2019 09:06:01: end of X-cor INFO @ Fri, 15 Feb 2019 09:06:01: #2 finished! INFO @ Fri, 15 Feb 2019 09:06:01: #2 predicted fragment length is 336 bps INFO @ Fri, 15 Feb 2019 09:06:01: #2 alternative fragment length(s) may be 13,286,310,336 bps INFO @ Fri, 15 Feb 2019 09:06:01: #2.2 Generate R script for model : ERX1420235.20_model.r INFO @ Fri, 15 Feb 2019 09:06:01: start X-correlation... INFO @ Fri, 15 Feb 2019 09:06:01: end of X-cor INFO @ Fri, 15 Feb 2019 09:06:01: #2 finished! INFO @ Fri, 15 Feb 2019 09:06:01: #2 predicted fragment length is 336 bps INFO @ Fri, 15 Feb 2019 09:06:01: #2 alternative fragment length(s) may be 13,286,310,336 bps INFO @ Fri, 15 Feb 2019 09:06:01: #2.2 Generate R script for model : ERX1420235.05_model.r INFO @ Fri, 15 Feb 2019 09:06:01: end of X-cor INFO @ Fri, 15 Feb 2019 09:06:01: #2 finished! INFO @ Fri, 15 Feb 2019 09:06:01: #2 predicted fragment length is 336 bps INFO @ Fri, 15 Feb 2019 09:06:01: #2 alternative fragment length(s) may be 13,286,310,336 bps INFO @ Fri, 15 Feb 2019 09:06:01: #2.2 Generate R script for model : ERX1420235.10_model.r INFO @ Fri, 15 Feb 2019 09:06:01: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:06:01: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:06:01: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:06:01: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:06:01: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:06:01: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:06:01: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:06:01: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:06:01: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:06:02: #4 Write output xls file... ERX1420235.10_peaks.xls INFO @ Fri, 15 Feb 2019 09:06:02: #4 Write peak in narrowPeak format file... ERX1420235.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:06:02: #4 Write summits bed file... ERX1420235.10_summits.bed INFO @ Fri, 15 Feb 2019 09:06:02: #4 Write output xls file... ERX1420235.05_peaks.xls INFO @ Fri, 15 Feb 2019 09:06:02: Done! INFO @ Fri, 15 Feb 2019 09:06:02: #4 Write peak in narrowPeak format file... ERX1420235.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:06:02: #4 Write output xls file... ERX1420235.20_peaks.xls INFO @ Fri, 15 Feb 2019 09:06:02: #4 Write summits bed file... ERX1420235.05_summits.bed INFO @ Fri, 15 Feb 2019 09:06:02: #4 Write peak in narrowPeak format file... ERX1420235.20_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:06:02: Done! INFO @ Fri, 15 Feb 2019 09:06:02: #4 Write summits bed file... ERX1420235.20_summits.bed INFO @ Fri, 15 Feb 2019 09:06:02: Done! pass1 - making usageList (13 chroms): 9 millis pass2 - checking and writing primary data (42 records, 4 fields): 6 millis pass1 - making usageList (15 chroms): 12 millis pass2 - checking and writing primary data (75 records, 4 fields): 10 millis CompletedMACS2peakCalling pass1 - making usageList (7 chroms): 10 millis CompletedMACS2peakCalling pass2 - checking and writing primary data (8 records, 4 fields): 6 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。