Job ID = 11633498 sra ファイルのダウンロード中... Completed: 46456K bytes transferred in 3 seconds (98763K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 768239 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236275/ERR1162868.sra Written 768239 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236275/ERR1162868.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:36 768239 reads; of these: 768239 (100.00%) were paired; of these: 130570 (17.00%) aligned concordantly 0 times 567080 (73.82%) aligned concordantly exactly 1 time 70589 (9.19%) aligned concordantly >1 times ---- 130570 pairs aligned concordantly 0 times; of these: 32246 (24.70%) aligned discordantly 1 time ---- 98324 pairs aligned 0 times concordantly or discordantly; of these: 196648 mates make up the pairs; of these: 184875 (94.01%) aligned 0 times 3867 (1.97%) aligned exactly 1 time 7906 (4.02%) aligned >1 times 87.97% overall alignment rate Time searching: 00:00:36 Overall time: 00:00:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 142313 / 669566 = 0.2125 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 08:58:09: # Command line: callpeak -t ERX1236275.bam -f BAM -g 12100000 -n ERX1236275.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1236275.20 # format = BAM # ChIP-seq file = ['ERX1236275.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:58:09: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:58:09: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:58:09: # Command line: callpeak -t ERX1236275.bam -f BAM -g 12100000 -n ERX1236275.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1236275.05 # format = BAM # ChIP-seq file = ['ERX1236275.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:58:09: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:58:09: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:58:09: # Command line: callpeak -t ERX1236275.bam -f BAM -g 12100000 -n ERX1236275.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1236275.10 # format = BAM # ChIP-seq file = ['ERX1236275.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:58:09: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:58:09: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:58:14: 1000000 INFO @ Fri, 15 Feb 2019 08:58:14: 1000000 INFO @ Fri, 15 Feb 2019 08:58:14: 1000000 INFO @ Fri, 15 Feb 2019 08:58:15: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:58:15: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:58:15: #1 total tags in treatment: 501838 INFO @ Fri, 15 Feb 2019 08:58:15: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:58:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:58:15: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:58:15: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:58:15: #1 total tags in treatment: 501838 INFO @ Fri, 15 Feb 2019 08:58:15: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:58:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:58:15: #1 tags after filtering in treatment: 486508 INFO @ Fri, 15 Feb 2019 08:58:15: #1 Redundant rate of treatment: 0.03 INFO @ Fri, 15 Feb 2019 08:58:15: #1 finished! INFO @ Fri, 15 Feb 2019 08:58:15: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:58:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:58:15: #1 tags after filtering in treatment: 486508 INFO @ Fri, 15 Feb 2019 08:58:15: #1 Redundant rate of treatment: 0.03 INFO @ Fri, 15 Feb 2019 08:58:15: #1 finished! INFO @ Fri, 15 Feb 2019 08:58:15: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:58:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:58:15: #2 number of paired peaks: 154 WARNING @ Fri, 15 Feb 2019 08:58:15: Fewer paired peaks (154) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 154 pairs to build model! INFO @ Fri, 15 Feb 2019 08:58:15: start model_add_line... INFO @ Fri, 15 Feb 2019 08:58:15: start X-correlation... INFO @ Fri, 15 Feb 2019 08:58:15: #2 number of paired peaks: 154 WARNING @ Fri, 15 Feb 2019 08:58:15: Fewer paired peaks (154) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 154 pairs to build model! INFO @ Fri, 15 Feb 2019 08:58:15: start model_add_line... INFO @ Fri, 15 Feb 2019 08:58:15: end of X-cor INFO @ Fri, 15 Feb 2019 08:58:15: #2 finished! INFO @ Fri, 15 Feb 2019 08:58:15: #2 predicted fragment length is 177 bps INFO @ Fri, 15 Feb 2019 08:58:15: #2 alternative fragment length(s) may be 3,44,102,123,145,177,214,276,351 bps INFO @ Fri, 15 Feb 2019 08:58:15: #2.2 Generate R script for model : ERX1236275.05_model.r INFO @ Fri, 15 Feb 2019 08:58:15: start X-correlation... INFO @ Fri, 15 Feb 2019 08:58:15: end of X-cor INFO @ Fri, 15 Feb 2019 08:58:15: #2 finished! INFO @ Fri, 15 Feb 2019 08:58:15: #2 predicted fragment length is 177 bps INFO @ Fri, 15 Feb 2019 08:58:15: #2 alternative fragment length(s) may be 3,44,102,123,145,177,214,276,351 bps INFO @ Fri, 15 Feb 2019 08:58:15: #2.2 Generate R script for model : ERX1236275.10_model.r INFO @ Fri, 15 Feb 2019 08:58:15: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:58:15: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:58:15: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:58:15: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:58:15: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:58:15: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:58:15: #1 total tags in treatment: 501838 INFO @ Fri, 15 Feb 2019 08:58:15: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:58:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:58:15: #1 tags after filtering in treatment: 486508 INFO @ Fri, 15 Feb 2019 08:58:15: #1 Redundant rate of treatment: 0.03 INFO @ Fri, 15 Feb 2019 08:58:15: #1 finished! INFO @ Fri, 15 Feb 2019 08:58:15: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:58:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:58:15: #2 number of paired peaks: 154 WARNING @ Fri, 15 Feb 2019 08:58:15: Fewer paired peaks (154) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 154 pairs to build model! INFO @ Fri, 15 Feb 2019 08:58:15: start model_add_line... INFO @ Fri, 15 Feb 2019 08:58:15: start X-correlation... INFO @ Fri, 15 Feb 2019 08:58:15: end of X-cor INFO @ Fri, 15 Feb 2019 08:58:15: #2 finished! INFO @ Fri, 15 Feb 2019 08:58:15: #2 predicted fragment length is 177 bps INFO @ Fri, 15 Feb 2019 08:58:15: #2 alternative fragment length(s) may be 3,44,102,123,145,177,214,276,351 bps INFO @ Fri, 15 Feb 2019 08:58:15: #2.2 Generate R script for model : ERX1236275.20_model.r INFO @ Fri, 15 Feb 2019 08:58:15: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:58:15: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:58:16: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:58:16: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:58:16: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:58:17: #4 Write output xls file... ERX1236275.10_peaks.xls INFO @ Fri, 15 Feb 2019 08:58:17: #4 Write peak in narrowPeak format file... ERX1236275.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 08:58:17: #4 Write summits bed file... ERX1236275.10_summits.bed INFO @ Fri, 15 Feb 2019 08:58:17: Done! INFO @ Fri, 15 Feb 2019 08:58:17: #4 Write output xls file... ERX1236275.05_peaks.xls INFO @ Fri, 15 Feb 2019 08:58:17: #4 Write peak in narrowPeak format file... ERX1236275.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 08:58:17: #4 Write summits bed file... ERX1236275.05_summits.bed INFO @ Fri, 15 Feb 2019 08:58:17: Done! pass1 - making usageList (16 chroms): 10 millis pass2 - checking and writing primary data (123 records, 4 fields): 8 millis INFO @ Fri, 15 Feb 2019 08:58:17: #4 Write output xls file... ERX1236275.20_peaks.xls INFO @ Fri, 15 Feb 2019 08:58:17: #4 Write peak in narrowPeak format file... ERX1236275.20_peaks.narrowPeak pass1 - making usageList (16 chroms): 11 millis INFO @ Fri, 15 Feb 2019 08:58:17: #4 Write summits bed file... ERX1236275.20_summits.bed pass2 - checking and writing primary data (269 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 08:58:17: Done! CompletedMACS2peakCalling pass1 - making usageList (15 chroms): 8 millis pass2 - checking and writing primary data (39 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。