Job ID = 11633493 sra ファイルのダウンロード中... Completed: 61977K bytes transferred in 3 seconds (135123K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 985859 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236270/ERR1162863.sra Written 985859 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236270/ERR1162863.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:29 985859 reads; of these: 985859 (100.00%) were paired; of these: 450271 (45.67%) aligned concordantly 0 times 499044 (50.62%) aligned concordantly exactly 1 time 36544 (3.71%) aligned concordantly >1 times ---- 450271 pairs aligned concordantly 0 times; of these: 29536 (6.56%) aligned discordantly 1 time ---- 420735 pairs aligned 0 times concordantly or discordantly; of these: 841470 mates make up the pairs; of these: 832526 (98.94%) aligned 0 times 4027 (0.48%) aligned exactly 1 time 4917 (0.58%) aligned >1 times 57.78% overall alignment rate Time searching: 00:00:29 Overall time: 00:00:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 74755 / 564612 = 0.1324 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 08:59:16: # Command line: callpeak -t ERX1236270.bam -f BAM -g 12100000 -n ERX1236270.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1236270.05 # format = BAM # ChIP-seq file = ['ERX1236270.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:59:16: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:59:16: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:59:16: # Command line: callpeak -t ERX1236270.bam -f BAM -g 12100000 -n ERX1236270.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1236270.10 # format = BAM # ChIP-seq file = ['ERX1236270.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:59:16: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:59:16: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:59:16: # Command line: callpeak -t ERX1236270.bam -f BAM -g 12100000 -n ERX1236270.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1236270.20 # format = BAM # ChIP-seq file = ['ERX1236270.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:59:16: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:59:16: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:59:22: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:59:22: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:59:22: #1 total tags in treatment: 465801 INFO @ Fri, 15 Feb 2019 08:59:22: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:59:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:59:22: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:59:22: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:59:22: #1 total tags in treatment: 465801 INFO @ Fri, 15 Feb 2019 08:59:22: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:59:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:59:22: #1 tags after filtering in treatment: 459768 INFO @ Fri, 15 Feb 2019 08:59:22: #1 Redundant rate of treatment: 0.01 INFO @ Fri, 15 Feb 2019 08:59:22: #1 finished! INFO @ Fri, 15 Feb 2019 08:59:22: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:59:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:59:22: #1 tags after filtering in treatment: 459768 INFO @ Fri, 15 Feb 2019 08:59:22: #1 Redundant rate of treatment: 0.01 INFO @ Fri, 15 Feb 2019 08:59:22: #1 finished! INFO @ Fri, 15 Feb 2019 08:59:22: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:59:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:59:22: #2 number of paired peaks: 21 WARNING @ Fri, 15 Feb 2019 08:59:22: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 08:59:22: Process for pairing-model is terminated! INFO @ Fri, 15 Feb 2019 08:59:22: #2 number of paired peaks: 21 WARNING @ Fri, 15 Feb 2019 08:59:22: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 08:59:22: Process for pairing-model is terminated! cat: ERX1236270.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: ERX1236270.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1236270.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1236270.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1236270.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1236270.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1236270.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1236270.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 08:59:22: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:59:22: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:59:22: #1 total tags in treatment: 465801 INFO @ Fri, 15 Feb 2019 08:59:22: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:59:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:59:22: #1 tags after filtering in treatment: 459768 INFO @ Fri, 15 Feb 2019 08:59:22: #1 Redundant rate of treatment: 0.01 INFO @ Fri, 15 Feb 2019 08:59:22: #1 finished! INFO @ Fri, 15 Feb 2019 08:59:22: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:59:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:59:22: #2 number of paired peaks: 21 WARNING @ Fri, 15 Feb 2019 08:59:22: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 08:59:22: Process for pairing-model is terminated! cat: ERX1236270.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1236270.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1236270.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1236270.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。