Job ID = 11633449 sra ファイルのダウンロード中... Completed: 192604K bytes transferred in 5 seconds (284474K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 3005706 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236226/ERR1162819.sra Written 3005706 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236226/ERR1162819.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:22 3005706 reads; of these: 3005706 (100.00%) were paired; of these: 162175 (5.40%) aligned concordantly 0 times 2605562 (86.69%) aligned concordantly exactly 1 time 237969 (7.92%) aligned concordantly >1 times ---- 162175 pairs aligned concordantly 0 times; of these: 16021 (9.88%) aligned discordantly 1 time ---- 146154 pairs aligned 0 times concordantly or discordantly; of these: 292308 mates make up the pairs; of these: 279454 (95.60%) aligned 0 times 8032 (2.75%) aligned exactly 1 time 4822 (1.65%) aligned >1 times 95.35% overall alignment rate Time searching: 00:02:22 Overall time: 00:02:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 691727 / 2856599 = 0.2422 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:03:50: # Command line: callpeak -t ERX1236226.bam -f BAM -g 12100000 -n ERX1236226.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1236226.20 # format = BAM # ChIP-seq file = ['ERX1236226.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:03:50: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:03:50: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:03:50: # Command line: callpeak -t ERX1236226.bam -f BAM -g 12100000 -n ERX1236226.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1236226.05 # format = BAM # ChIP-seq file = ['ERX1236226.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:03:50: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:03:50: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:03:50: # Command line: callpeak -t ERX1236226.bam -f BAM -g 12100000 -n ERX1236226.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1236226.10 # format = BAM # ChIP-seq file = ['ERX1236226.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:03:50: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:03:50: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:03:57: 1000000 INFO @ Fri, 15 Feb 2019 09:03:59: 1000000 INFO @ Fri, 15 Feb 2019 09:03:59: 1000000 INFO @ Fri, 15 Feb 2019 09:04:05: 2000000 INFO @ Fri, 15 Feb 2019 09:04:08: 2000000 INFO @ Fri, 15 Feb 2019 09:04:08: 2000000 INFO @ Fri, 15 Feb 2019 09:04:12: 3000000 INFO @ Fri, 15 Feb 2019 09:04:16: 3000000 INFO @ Fri, 15 Feb 2019 09:04:16: 3000000 INFO @ Fri, 15 Feb 2019 09:04:19: 4000000 INFO @ Fri, 15 Feb 2019 09:04:21: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:04:21: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:04:21: #1 total tags in treatment: 2155492 INFO @ Fri, 15 Feb 2019 09:04:21: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:04:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:04:21: #1 tags after filtering in treatment: 1950211 INFO @ Fri, 15 Feb 2019 09:04:21: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 15 Feb 2019 09:04:21: #1 finished! INFO @ Fri, 15 Feb 2019 09:04:21: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:04:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:04:21: #2 number of paired peaks: 151 WARNING @ Fri, 15 Feb 2019 09:04:21: Fewer paired peaks (151) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 151 pairs to build model! INFO @ Fri, 15 Feb 2019 09:04:21: start model_add_line... INFO @ Fri, 15 Feb 2019 09:04:21: start X-correlation... INFO @ Fri, 15 Feb 2019 09:04:21: end of X-cor INFO @ Fri, 15 Feb 2019 09:04:21: #2 finished! INFO @ Fri, 15 Feb 2019 09:04:21: #2 predicted fragment length is 120 bps INFO @ Fri, 15 Feb 2019 09:04:21: #2 alternative fragment length(s) may be 4,52,87,120,133,159,220 bps INFO @ Fri, 15 Feb 2019 09:04:21: #2.2 Generate R script for model : ERX1236226.20_model.r INFO @ Fri, 15 Feb 2019 09:04:21: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:04:21: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:04:23: 4000000 INFO @ Fri, 15 Feb 2019 09:04:23: 4000000 INFO @ Fri, 15 Feb 2019 09:04:25: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:04:25: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:04:25: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:04:25: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:04:25: #1 total tags in treatment: 2155492 INFO @ Fri, 15 Feb 2019 09:04:25: #1 total tags in treatment: 2155492 INFO @ Fri, 15 Feb 2019 09:04:25: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:04:25: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:04:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:04:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:04:25: #1 tags after filtering in treatment: 1950211 INFO @ Fri, 15 Feb 2019 09:04:25: #1 tags after filtering in treatment: 1950211 INFO @ Fri, 15 Feb 2019 09:04:25: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 15 Feb 2019 09:04:25: #1 finished! INFO @ Fri, 15 Feb 2019 09:04:25: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 15 Feb 2019 09:04:25: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:04:25: #1 finished! INFO @ Fri, 15 Feb 2019 09:04:25: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:04:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:04:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:04:26: #2 number of paired peaks: 151 WARNING @ Fri, 15 Feb 2019 09:04:26: Fewer paired peaks (151) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 151 pairs to build model! INFO @ Fri, 15 Feb 2019 09:04:26: start model_add_line... INFO @ Fri, 15 Feb 2019 09:04:26: #2 number of paired peaks: 151 WARNING @ Fri, 15 Feb 2019 09:04:26: Fewer paired peaks (151) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 151 pairs to build model! INFO @ Fri, 15 Feb 2019 09:04:26: start model_add_line... INFO @ Fri, 15 Feb 2019 09:04:26: start X-correlation... INFO @ Fri, 15 Feb 2019 09:04:26: end of X-cor INFO @ Fri, 15 Feb 2019 09:04:26: #2 finished! INFO @ Fri, 15 Feb 2019 09:04:26: #2 predicted fragment length is 120 bps INFO @ Fri, 15 Feb 2019 09:04:26: #2 alternative fragment length(s) may be 4,52,87,120,133,159,220 bps INFO @ Fri, 15 Feb 2019 09:04:26: #2.2 Generate R script for model : ERX1236226.05_model.r INFO @ Fri, 15 Feb 2019 09:04:26: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:04:26: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:04:26: start X-correlation... INFO @ Fri, 15 Feb 2019 09:04:26: end of X-cor INFO @ Fri, 15 Feb 2019 09:04:26: #2 finished! INFO @ Fri, 15 Feb 2019 09:04:26: #2 predicted fragment length is 120 bps INFO @ Fri, 15 Feb 2019 09:04:26: #2 alternative fragment length(s) may be 4,52,87,120,133,159,220 bps INFO @ Fri, 15 Feb 2019 09:04:26: #2.2 Generate R script for model : ERX1236226.10_model.r INFO @ Fri, 15 Feb 2019 09:04:26: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:04:26: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:04:27: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:04:29: #4 Write output xls file... ERX1236226.20_peaks.xls INFO @ Fri, 15 Feb 2019 09:04:29: #4 Write peak in narrowPeak format file... ERX1236226.20_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:04:29: #4 Write summits bed file... ERX1236226.20_summits.bed INFO @ Fri, 15 Feb 2019 09:04:29: Done! pass1 - making usageList (16 chroms): 3 millis pass2 - checking and writing primary data (155 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:04:31: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:04:31: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:04:33: #4 Write output xls file... ERX1236226.10_peaks.xls INFO @ Fri, 15 Feb 2019 09:04:33: #4 Write peak in narrowPeak format file... ERX1236226.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:04:33: #4 Write summits bed file... ERX1236226.10_summits.bed INFO @ Fri, 15 Feb 2019 09:04:33: Done! pass1 - making usageList (16 chroms): 3 millis pass2 - checking and writing primary data (351 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:04:33: #4 Write output xls file... ERX1236226.05_peaks.xls INFO @ Fri, 15 Feb 2019 09:04:33: #4 Write peak in narrowPeak format file... ERX1236226.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:04:33: #4 Write summits bed file... ERX1236226.05_summits.bed INFO @ Fri, 15 Feb 2019 09:04:33: Done! pass1 - making usageList (16 chroms): 3 millis pass2 - checking and writing primary data (735 records, 4 fields): 6 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。