Job ID = 11633443 sra ファイルのダウンロード中... Completed: 33805K bytes transferred in 3 seconds (81070K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 474706 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236220/ERR1162813.sra Written 474706 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236220/ERR1162813.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:22 474706 reads; of these: 474706 (100.00%) were paired; of these: 28652 (6.04%) aligned concordantly 0 times 392342 (82.65%) aligned concordantly exactly 1 time 53712 (11.31%) aligned concordantly >1 times ---- 28652 pairs aligned concordantly 0 times; of these: 2485 (8.67%) aligned discordantly 1 time ---- 26167 pairs aligned 0 times concordantly or discordantly; of these: 52334 mates make up the pairs; of these: 48373 (92.43%) aligned 0 times 2700 (5.16%) aligned exactly 1 time 1261 (2.41%) aligned >1 times 94.90% overall alignment rate Time searching: 00:00:23 Overall time: 00:00:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 13183 / 447893 = 0.0294 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 08:53:48: # Command line: callpeak -t ERX1236220.bam -f BAM -g 12100000 -n ERX1236220.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1236220.05 # format = BAM # ChIP-seq file = ['ERX1236220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:53:48: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:53:48: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:53:48: # Command line: callpeak -t ERX1236220.bam -f BAM -g 12100000 -n ERX1236220.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1236220.10 # format = BAM # ChIP-seq file = ['ERX1236220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:53:48: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:53:48: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:53:48: # Command line: callpeak -t ERX1236220.bam -f BAM -g 12100000 -n ERX1236220.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1236220.20 # format = BAM # ChIP-seq file = ['ERX1236220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:53:48: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:53:48: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:53:53: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:53:53: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:53:53: #1 total tags in treatment: 432899 INFO @ Fri, 15 Feb 2019 08:53:53: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:53:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:53:53: #1 tags after filtering in treatment: 418108 INFO @ Fri, 15 Feb 2019 08:53:53: #1 Redundant rate of treatment: 0.03 INFO @ Fri, 15 Feb 2019 08:53:53: #1 finished! INFO @ Fri, 15 Feb 2019 08:53:53: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:53:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:53:53: #2 number of paired peaks: 291 WARNING @ Fri, 15 Feb 2019 08:53:53: Fewer paired peaks (291) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 291 pairs to build model! INFO @ Fri, 15 Feb 2019 08:53:53: start model_add_line... INFO @ Fri, 15 Feb 2019 08:53:53: start X-correlation... INFO @ Fri, 15 Feb 2019 08:53:53: end of X-cor INFO @ Fri, 15 Feb 2019 08:53:53: #2 finished! INFO @ Fri, 15 Feb 2019 08:53:53: #2 predicted fragment length is 196 bps INFO @ Fri, 15 Feb 2019 08:53:53: #2 alternative fragment length(s) may be 4,152,196,213,250 bps INFO @ Fri, 15 Feb 2019 08:53:53: #2.2 Generate R script for model : ERX1236220.20_model.r INFO @ Fri, 15 Feb 2019 08:53:53: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:53:53: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:53:53: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:53:53: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:53:53: #1 total tags in treatment: 432899 INFO @ Fri, 15 Feb 2019 08:53:53: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:53:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:53:53: #1 tags after filtering in treatment: 418108 INFO @ Fri, 15 Feb 2019 08:53:53: #1 Redundant rate of treatment: 0.03 INFO @ Fri, 15 Feb 2019 08:53:53: #1 finished! INFO @ Fri, 15 Feb 2019 08:53:53: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:53:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:53:53: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:53:53: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:53:53: #1 total tags in treatment: 432899 INFO @ Fri, 15 Feb 2019 08:53:53: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:53:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:53:53: #2 number of paired peaks: 291 WARNING @ Fri, 15 Feb 2019 08:53:53: Fewer paired peaks (291) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 291 pairs to build model! INFO @ Fri, 15 Feb 2019 08:53:53: start model_add_line... INFO @ Fri, 15 Feb 2019 08:53:53: start X-correlation... INFO @ Fri, 15 Feb 2019 08:53:53: #1 tags after filtering in treatment: 418108 INFO @ Fri, 15 Feb 2019 08:53:53: #1 Redundant rate of treatment: 0.03 INFO @ Fri, 15 Feb 2019 08:53:53: #1 finished! INFO @ Fri, 15 Feb 2019 08:53:53: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:53:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:53:53: end of X-cor INFO @ Fri, 15 Feb 2019 08:53:53: #2 finished! INFO @ Fri, 15 Feb 2019 08:53:53: #2 predicted fragment length is 196 bps INFO @ Fri, 15 Feb 2019 08:53:53: #2 alternative fragment length(s) may be 4,152,196,213,250 bps INFO @ Fri, 15 Feb 2019 08:53:53: #2.2 Generate R script for model : ERX1236220.05_model.r INFO @ Fri, 15 Feb 2019 08:53:53: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:53:53: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:53:53: #2 number of paired peaks: 291 WARNING @ Fri, 15 Feb 2019 08:53:53: Fewer paired peaks (291) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 291 pairs to build model! INFO @ Fri, 15 Feb 2019 08:53:53: start model_add_line... INFO @ Fri, 15 Feb 2019 08:53:53: start X-correlation... INFO @ Fri, 15 Feb 2019 08:53:53: end of X-cor INFO @ Fri, 15 Feb 2019 08:53:53: #2 finished! INFO @ Fri, 15 Feb 2019 08:53:53: #2 predicted fragment length is 196 bps INFO @ Fri, 15 Feb 2019 08:53:53: #2 alternative fragment length(s) may be 4,152,196,213,250 bps INFO @ Fri, 15 Feb 2019 08:53:53: #2.2 Generate R script for model : ERX1236220.10_model.r INFO @ Fri, 15 Feb 2019 08:53:53: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:53:53: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:53:54: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:53:54: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:53:54: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:53:55: #4 Write output xls file... ERX1236220.20_peaks.xls INFO @ Fri, 15 Feb 2019 08:53:55: #4 Write peak in narrowPeak format file... ERX1236220.20_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 08:53:55: #4 Write summits bed file... ERX1236220.20_summits.bed INFO @ Fri, 15 Feb 2019 08:53:55: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (73 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 08:53:55: #4 Write output xls file... ERX1236220.05_peaks.xls INFO @ Fri, 15 Feb 2019 08:53:55: #4 Write peak in narrowPeak format file... ERX1236220.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 08:53:55: #4 Write summits bed file... ERX1236220.05_summits.bed INFO @ Fri, 15 Feb 2019 08:53:55: Done! INFO @ Fri, 15 Feb 2019 08:53:55: #4 Write output xls file... ERX1236220.10_peaks.xls INFO @ Fri, 15 Feb 2019 08:53:55: #4 Write peak in narrowPeak format file... ERX1236220.10_peaks.narrowPeak pass1 - making usageList (16 chroms): 3 millis INFO @ Fri, 15 Feb 2019 08:53:55: #4 Write summits bed file... ERX1236220.10_summits.bed pass2 - checking and writing primary data (318 records, 4 fields): 4 millis INFO @ Fri, 15 Feb 2019 08:53:55: Done! CompletedMACS2peakCalling pass1 - making usageList (16 chroms): 2 millis pass2 - checking and writing primary data (197 records, 4 fields): 4 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。