Job ID = 11633441 sra ファイルのダウンロード中... Completed: 160853K bytes transferred in 4 seconds (292351K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 2365677 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236218/ERR1162811.sra Written 2365677 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236218/ERR1162811.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:51 2365677 reads; of these: 2365677 (100.00%) were paired; of these: 282266 (11.93%) aligned concordantly 0 times 1868559 (78.99%) aligned concordantly exactly 1 time 214852 (9.08%) aligned concordantly >1 times ---- 282266 pairs aligned concordantly 0 times; of these: 95591 (33.87%) aligned discordantly 1 time ---- 186675 pairs aligned 0 times concordantly or discordantly; of these: 373350 mates make up the pairs; of these: 327226 (87.65%) aligned 0 times 20721 (5.55%) aligned exactly 1 time 25403 (6.80%) aligned >1 times 93.08% overall alignment rate Time searching: 00:01:51 Overall time: 00:01:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 427011 / 2166048 = 0.1971 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 08:59:38: # Command line: callpeak -t ERX1236218.bam -f BAM -g 12100000 -n ERX1236218.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1236218.10 # format = BAM # ChIP-seq file = ['ERX1236218.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:59:38: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:59:38: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:59:38: # Command line: callpeak -t ERX1236218.bam -f BAM -g 12100000 -n ERX1236218.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1236218.05 # format = BAM # ChIP-seq file = ['ERX1236218.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:59:38: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:59:38: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:59:38: # Command line: callpeak -t ERX1236218.bam -f BAM -g 12100000 -n ERX1236218.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1236218.20 # format = BAM # ChIP-seq file = ['ERX1236218.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:59:38: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:59:38: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:59:44: 1000000 INFO @ Fri, 15 Feb 2019 08:59:44: 1000000 INFO @ Fri, 15 Feb 2019 08:59:44: 1000000 INFO @ Fri, 15 Feb 2019 08:59:50: 2000000 INFO @ Fri, 15 Feb 2019 08:59:50: 2000000 INFO @ Fri, 15 Feb 2019 08:59:50: 2000000 INFO @ Fri, 15 Feb 2019 08:59:56: 3000000 INFO @ Fri, 15 Feb 2019 08:59:56: 3000000 INFO @ Fri, 15 Feb 2019 08:59:56: 3000000 INFO @ Fri, 15 Feb 2019 08:59:59: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:59:59: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:59:59: #1 total tags in treatment: 1665988 INFO @ Fri, 15 Feb 2019 08:59:59: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:59:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:59:59: #1 tags after filtering in treatment: 1517322 INFO @ Fri, 15 Feb 2019 08:59:59: #1 Redundant rate of treatment: 0.09 INFO @ Fri, 15 Feb 2019 08:59:59: #1 finished! INFO @ Fri, 15 Feb 2019 08:59:59: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:59:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:59:59: #2 number of paired peaks: 234 WARNING @ Fri, 15 Feb 2019 08:59:59: Fewer paired peaks (234) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 234 pairs to build model! INFO @ Fri, 15 Feb 2019 08:59:59: start model_add_line... INFO @ Fri, 15 Feb 2019 08:59:59: start X-correlation... INFO @ Fri, 15 Feb 2019 08:59:59: end of X-cor INFO @ Fri, 15 Feb 2019 08:59:59: #2 finished! INFO @ Fri, 15 Feb 2019 08:59:59: #2 predicted fragment length is 210 bps INFO @ Fri, 15 Feb 2019 08:59:59: #2 alternative fragment length(s) may be 4,195,210 bps INFO @ Fri, 15 Feb 2019 08:59:59: #2.2 Generate R script for model : ERX1236218.20_model.r INFO @ Fri, 15 Feb 2019 08:59:59: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:59:59: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:59:59: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:59:59: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:59:59: #1 total tags in treatment: 1665988 INFO @ Fri, 15 Feb 2019 08:59:59: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:59:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:59:59: #1 tags after filtering in treatment: 1517322 INFO @ Fri, 15 Feb 2019 08:59:59: #1 Redundant rate of treatment: 0.09 INFO @ Fri, 15 Feb 2019 08:59:59: #1 finished! INFO @ Fri, 15 Feb 2019 08:59:59: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:59:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:00:00: #2 number of paired peaks: 234 WARNING @ Fri, 15 Feb 2019 09:00:00: Fewer paired peaks (234) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 234 pairs to build model! INFO @ Fri, 15 Feb 2019 09:00:00: start model_add_line... INFO @ Fri, 15 Feb 2019 09:00:00: start X-correlation... INFO @ Fri, 15 Feb 2019 09:00:00: end of X-cor INFO @ Fri, 15 Feb 2019 09:00:00: #2 finished! INFO @ Fri, 15 Feb 2019 09:00:00: #2 predicted fragment length is 210 bps INFO @ Fri, 15 Feb 2019 09:00:00: #2 alternative fragment length(s) may be 4,195,210 bps INFO @ Fri, 15 Feb 2019 09:00:00: #2.2 Generate R script for model : ERX1236218.05_model.r INFO @ Fri, 15 Feb 2019 09:00:00: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:00:00: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:00:00: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:00:00: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:00:00: #1 total tags in treatment: 1665988 INFO @ Fri, 15 Feb 2019 09:00:00: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:00:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:00:00: #1 tags after filtering in treatment: 1517322 INFO @ Fri, 15 Feb 2019 09:00:00: #1 Redundant rate of treatment: 0.09 INFO @ Fri, 15 Feb 2019 09:00:00: #1 finished! INFO @ Fri, 15 Feb 2019 09:00:00: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:00:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:00:01: #2 number of paired peaks: 234 WARNING @ Fri, 15 Feb 2019 09:00:01: Fewer paired peaks (234) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 234 pairs to build model! INFO @ Fri, 15 Feb 2019 09:00:01: start model_add_line... INFO @ Fri, 15 Feb 2019 09:00:01: start X-correlation... INFO @ Fri, 15 Feb 2019 09:00:01: end of X-cor INFO @ Fri, 15 Feb 2019 09:00:01: #2 finished! INFO @ Fri, 15 Feb 2019 09:00:01: #2 predicted fragment length is 210 bps INFO @ Fri, 15 Feb 2019 09:00:01: #2 alternative fragment length(s) may be 4,195,210 bps INFO @ Fri, 15 Feb 2019 09:00:01: #2.2 Generate R script for model : ERX1236218.10_model.r INFO @ Fri, 15 Feb 2019 09:00:01: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:00:01: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:00:04: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:00:05: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:00:06: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:00:06: #4 Write output xls file... ERX1236218.20_peaks.xls INFO @ Fri, 15 Feb 2019 09:00:06: #4 Write peak in narrowPeak format file... ERX1236218.20_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:00:06: #4 Write summits bed file... ERX1236218.20_summits.bed INFO @ Fri, 15 Feb 2019 09:00:06: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (257 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:00:07: #4 Write output xls file... ERX1236218.05_peaks.xls INFO @ Fri, 15 Feb 2019 09:00:07: #4 Write peak in narrowPeak format file... ERX1236218.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:00:07: #4 Write summits bed file... ERX1236218.05_summits.bed INFO @ Fri, 15 Feb 2019 09:00:07: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (588 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:00:08: #4 Write output xls file... ERX1236218.10_peaks.xls INFO @ Fri, 15 Feb 2019 09:00:08: #4 Write peak in narrowPeak format file... ERX1236218.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:00:08: #4 Write summits bed file... ERX1236218.10_summits.bed INFO @ Fri, 15 Feb 2019 09:00:08: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (390 records, 4 fields): 4 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。