Job ID = 11633437 sra ファイルのダウンロード中... Completed: 77737K bytes transferred in 4 seconds (150283K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 1307261 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236214/ERR1162807.sra Written 1307261 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236214/ERR1162807.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:00 1307261 reads; of these: 1307261 (100.00%) were paired; of these: 175997 (13.46%) aligned concordantly 0 times 969739 (74.18%) aligned concordantly exactly 1 time 161525 (12.36%) aligned concordantly >1 times ---- 175997 pairs aligned concordantly 0 times; of these: 37297 (21.19%) aligned discordantly 1 time ---- 138700 pairs aligned 0 times concordantly or discordantly; of these: 277400 mates make up the pairs; of these: 257960 (92.99%) aligned 0 times 5415 (1.95%) aligned exactly 1 time 14025 (5.06%) aligned >1 times 90.13% overall alignment rate Time searching: 00:01:00 Overall time: 00:01:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 100076 / 1168278 = 0.0857 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 08:57:09: # Command line: callpeak -t ERX1236214.bam -f BAM -g 12100000 -n ERX1236214.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1236214.10 # format = BAM # ChIP-seq file = ['ERX1236214.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:57:09: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:57:09: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:57:09: # Command line: callpeak -t ERX1236214.bam -f BAM -g 12100000 -n ERX1236214.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1236214.05 # format = BAM # ChIP-seq file = ['ERX1236214.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:57:09: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:57:09: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:57:09: # Command line: callpeak -t ERX1236214.bam -f BAM -g 12100000 -n ERX1236214.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1236214.20 # format = BAM # ChIP-seq file = ['ERX1236214.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:57:09: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:57:09: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:57:15: 1000000 INFO @ Fri, 15 Feb 2019 08:57:15: 1000000 INFO @ Fri, 15 Feb 2019 08:57:15: 1000000 INFO @ Fri, 15 Feb 2019 08:57:21: 2000000 INFO @ Fri, 15 Feb 2019 08:57:21: 2000000 INFO @ Fri, 15 Feb 2019 08:57:21: 2000000 INFO @ Fri, 15 Feb 2019 08:57:22: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:57:22: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:57:22: #1 total tags in treatment: 1034378 INFO @ Fri, 15 Feb 2019 08:57:22: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:57:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:57:22: #1 tags after filtering in treatment: 949091 INFO @ Fri, 15 Feb 2019 08:57:22: #1 Redundant rate of treatment: 0.08 INFO @ Fri, 15 Feb 2019 08:57:22: #1 finished! INFO @ Fri, 15 Feb 2019 08:57:22: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:57:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:57:22: #2 number of paired peaks: 136 WARNING @ Fri, 15 Feb 2019 08:57:22: Fewer paired peaks (136) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 136 pairs to build model! INFO @ Fri, 15 Feb 2019 08:57:22: start model_add_line... INFO @ Fri, 15 Feb 2019 08:57:22: start X-correlation... INFO @ Fri, 15 Feb 2019 08:57:22: end of X-cor INFO @ Fri, 15 Feb 2019 08:57:22: #2 finished! INFO @ Fri, 15 Feb 2019 08:57:22: #2 predicted fragment length is 168 bps INFO @ Fri, 15 Feb 2019 08:57:22: #2 alternative fragment length(s) may be 4,142,168,219,259 bps INFO @ Fri, 15 Feb 2019 08:57:22: #2.2 Generate R script for model : ERX1236214.20_model.r INFO @ Fri, 15 Feb 2019 08:57:22: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:57:22: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:57:22: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:57:22: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:57:22: #1 total tags in treatment: 1034378 INFO @ Fri, 15 Feb 2019 08:57:22: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:57:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:57:22: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:57:22: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:57:22: #1 total tags in treatment: 1034378 INFO @ Fri, 15 Feb 2019 08:57:22: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:57:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:57:22: #1 tags after filtering in treatment: 949091 INFO @ Fri, 15 Feb 2019 08:57:22: #1 Redundant rate of treatment: 0.08 INFO @ Fri, 15 Feb 2019 08:57:22: #1 finished! INFO @ Fri, 15 Feb 2019 08:57:22: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:57:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:57:22: #1 tags after filtering in treatment: 949091 INFO @ Fri, 15 Feb 2019 08:57:22: #1 Redundant rate of treatment: 0.08 INFO @ Fri, 15 Feb 2019 08:57:22: #1 finished! INFO @ Fri, 15 Feb 2019 08:57:22: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:57:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:57:22: #2 number of paired peaks: 136 WARNING @ Fri, 15 Feb 2019 08:57:22: Fewer paired peaks (136) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 136 pairs to build model! INFO @ Fri, 15 Feb 2019 08:57:22: start model_add_line... INFO @ Fri, 15 Feb 2019 08:57:22: start X-correlation... INFO @ Fri, 15 Feb 2019 08:57:22: #2 number of paired peaks: 136 WARNING @ Fri, 15 Feb 2019 08:57:22: Fewer paired peaks (136) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 136 pairs to build model! INFO @ Fri, 15 Feb 2019 08:57:22: start model_add_line... INFO @ Fri, 15 Feb 2019 08:57:22: end of X-cor INFO @ Fri, 15 Feb 2019 08:57:22: #2 finished! INFO @ Fri, 15 Feb 2019 08:57:22: #2 predicted fragment length is 168 bps INFO @ Fri, 15 Feb 2019 08:57:22: #2 alternative fragment length(s) may be 4,142,168,219,259 bps INFO @ Fri, 15 Feb 2019 08:57:22: #2.2 Generate R script for model : ERX1236214.10_model.r INFO @ Fri, 15 Feb 2019 08:57:22: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:57:22: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:57:22: start X-correlation... INFO @ Fri, 15 Feb 2019 08:57:22: end of X-cor INFO @ Fri, 15 Feb 2019 08:57:22: #2 finished! INFO @ Fri, 15 Feb 2019 08:57:22: #2 predicted fragment length is 168 bps INFO @ Fri, 15 Feb 2019 08:57:22: #2 alternative fragment length(s) may be 4,142,168,219,259 bps INFO @ Fri, 15 Feb 2019 08:57:22: #2.2 Generate R script for model : ERX1236214.05_model.r INFO @ Fri, 15 Feb 2019 08:57:22: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:57:22: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:57:24: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:57:25: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:57:25: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:57:26: #4 Write output xls file... ERX1236214.20_peaks.xls INFO @ Fri, 15 Feb 2019 08:57:26: #4 Write peak in narrowPeak format file... ERX1236214.20_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 08:57:26: #4 Write summits bed file... ERX1236214.20_summits.bed INFO @ Fri, 15 Feb 2019 08:57:26: Done! pass1 - making usageList (16 chroms): 2 millis pass2 - checking and writing primary data (110 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 08:57:26: #4 Write output xls file... ERX1236214.10_peaks.xls INFO @ Fri, 15 Feb 2019 08:57:26: #4 Write peak in narrowPeak format file... ERX1236214.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 08:57:26: #4 Write summits bed file... ERX1236214.10_summits.bed INFO @ Fri, 15 Feb 2019 08:57:26: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (258 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 08:57:26: #4 Write output xls file... ERX1236214.05_peaks.xls INFO @ Fri, 15 Feb 2019 08:57:26: #4 Write peak in narrowPeak format file... ERX1236214.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 08:57:26: #4 Write summits bed file... ERX1236214.05_summits.bed INFO @ Fri, 15 Feb 2019 08:57:26: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (391 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。