Job ID = 11633430 sra ファイルのダウンロード中... Completed: 1213K bytes transferred in 2 seconds (4805K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 16665 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236207/ERR1162800.sra Written 16665 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236207/ERR1162800.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:01 16665 reads; of these: 16665 (100.00%) were paired; of these: 1742 (10.45%) aligned concordantly 0 times 13065 (78.40%) aligned concordantly exactly 1 time 1858 (11.15%) aligned concordantly >1 times ---- 1742 pairs aligned concordantly 0 times; of these: 450 (25.83%) aligned discordantly 1 time ---- 1292 pairs aligned 0 times concordantly or discordantly; of these: 2584 mates make up the pairs; of these: 2076 (80.34%) aligned 0 times 310 (12.00%) aligned exactly 1 time 198 (7.66%) aligned >1 times 93.77% overall alignment rate Time searching: 00:00:01 Overall time: 00:00:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 84 / 14970 = 0.0056 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 08:52:07: # Command line: callpeak -t ERX1236207.bam -f BAM -g 12100000 -n ERX1236207.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1236207.20 # format = BAM # ChIP-seq file = ['ERX1236207.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:52:07: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:52:07: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:52:07: # Command line: callpeak -t ERX1236207.bam -f BAM -g 12100000 -n ERX1236207.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1236207.10 # format = BAM # ChIP-seq file = ['ERX1236207.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:52:07: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:52:07: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:52:07: # Command line: callpeak -t ERX1236207.bam -f BAM -g 12100000 -n ERX1236207.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1236207.05 # format = BAM # ChIP-seq file = ['ERX1236207.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:52:07: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:52:07: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:52:07: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:52:07: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:52:07: #1 total tags in treatment: 14839 INFO @ Fri, 15 Feb 2019 08:52:07: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:52:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:52:07: #1 tags after filtering in treatment: 14822 INFO @ Fri, 15 Feb 2019 08:52:07: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 08:52:07: #1 finished! INFO @ Fri, 15 Feb 2019 08:52:07: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:52:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:52:07: #2 number of paired peaks: 275 WARNING @ Fri, 15 Feb 2019 08:52:07: Fewer paired peaks (275) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 275 pairs to build model! INFO @ Fri, 15 Feb 2019 08:52:07: start model_add_line... INFO @ Fri, 15 Feb 2019 08:52:07: start X-correlation... INFO @ Fri, 15 Feb 2019 08:52:07: end of X-cor INFO @ Fri, 15 Feb 2019 08:52:07: #2 finished! INFO @ Fri, 15 Feb 2019 08:52:07: #2 predicted fragment length is 295 bps INFO @ Fri, 15 Feb 2019 08:52:07: #2 alternative fragment length(s) may be 6,63,86,108,130,206,230,295,325,344,366,393,417,445,469,493,540,566 bps INFO @ Fri, 15 Feb 2019 08:52:07: #2.2 Generate R script for model : ERX1236207.20_model.r INFO @ Fri, 15 Feb 2019 08:52:07: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:52:07: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:52:07: #1 total tags in treatment: 14839 INFO @ Fri, 15 Feb 2019 08:52:07: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:52:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:52:07: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:52:07: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:52:07: #1 total tags in treatment: 14839 INFO @ Fri, 15 Feb 2019 08:52:07: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:52:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:52:07: #1 tags after filtering in treatment: 14822 INFO @ Fri, 15 Feb 2019 08:52:07: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 08:52:07: #1 finished! INFO @ Fri, 15 Feb 2019 08:52:07: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:52:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:52:07: #1 tags after filtering in treatment: 14822 INFO @ Fri, 15 Feb 2019 08:52:07: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 08:52:07: #1 finished! INFO @ Fri, 15 Feb 2019 08:52:07: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:52:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:52:07: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:52:07: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:52:07: #2 number of paired peaks: 275 WARNING @ Fri, 15 Feb 2019 08:52:07: Fewer paired peaks (275) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 275 pairs to build model! INFO @ Fri, 15 Feb 2019 08:52:07: start model_add_line... INFO @ Fri, 15 Feb 2019 08:52:07: start X-correlation... INFO @ Fri, 15 Feb 2019 08:52:07: #2 number of paired peaks: 275 WARNING @ Fri, 15 Feb 2019 08:52:07: Fewer paired peaks (275) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 275 pairs to build model! INFO @ Fri, 15 Feb 2019 08:52:07: start model_add_line... INFO @ Fri, 15 Feb 2019 08:52:07: start X-correlation... INFO @ Fri, 15 Feb 2019 08:52:07: end of X-cor INFO @ Fri, 15 Feb 2019 08:52:07: #2 finished! INFO @ Fri, 15 Feb 2019 08:52:07: #2 predicted fragment length is 295 bps INFO @ Fri, 15 Feb 2019 08:52:07: #2 alternative fragment length(s) may be 6,63,86,108,130,206,230,295,325,344,366,393,417,445,469,493,540,566 bps INFO @ Fri, 15 Feb 2019 08:52:07: end of X-cor INFO @ Fri, 15 Feb 2019 08:52:07: #2.2 Generate R script for model : ERX1236207.10_model.r INFO @ Fri, 15 Feb 2019 08:52:07: #2 finished! INFO @ Fri, 15 Feb 2019 08:52:07: #2 predicted fragment length is 295 bps INFO @ Fri, 15 Feb 2019 08:52:07: #2 alternative fragment length(s) may be 6,63,86,108,130,206,230,295,325,344,366,393,417,445,469,493,540,566 bps INFO @ Fri, 15 Feb 2019 08:52:07: #2.2 Generate R script for model : ERX1236207.05_model.r INFO @ Fri, 15 Feb 2019 08:52:07: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:52:07: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:52:07: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:52:07: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:52:07: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:52:07: #4 Write output xls file... ERX1236207.20_peaks.xls INFO @ Fri, 15 Feb 2019 08:52:07: #4 Write peak in narrowPeak format file... ERX1236207.20_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 08:52:07: #4 Write summits bed file... ERX1236207.20_summits.bed INFO @ Fri, 15 Feb 2019 08:52:07: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:52:07: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:52:07: Done! INFO @ Fri, 15 Feb 2019 08:52:07: #4 Write output xls file... ERX1236207.10_peaks.xls INFO @ Fri, 15 Feb 2019 08:52:07: #4 Write output xls file... ERX1236207.05_peaks.xls INFO @ Fri, 15 Feb 2019 08:52:07: #4 Write peak in narrowPeak format file... ERX1236207.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 08:52:07: #4 Write peak in narrowPeak format file... ERX1236207.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 08:52:07: #4 Write summits bed file... ERX1236207.10_summits.bed INFO @ Fri, 15 Feb 2019 08:52:07: #4 Write summits bed file... ERX1236207.05_summits.bed INFO @ Fri, 15 Feb 2019 08:52:07: Done! INFO @ Fri, 15 Feb 2019 08:52:07: Done! pass1 - making usageList (0 chroms): 11 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) BedGraph に変換しました。 BigWig に変換中... pass1 - making usageList (0 chroms): 17 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 10 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling BigWig に変換しました。