Job ID = 11633414 sra ファイルのダウンロード中... Completed: 242659K bytes transferred in 5 seconds (352129K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236191/ERR1162784.sra Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236191/ERR1162784.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:06 4000000 reads; of these: 4000000 (100.00%) were paired; of these: 474952 (11.87%) aligned concordantly 0 times 3164995 (79.12%) aligned concordantly exactly 1 time 360053 (9.00%) aligned concordantly >1 times ---- 474952 pairs aligned concordantly 0 times; of these: 23132 (4.87%) aligned discordantly 1 time ---- 451820 pairs aligned 0 times concordantly or discordantly; of these: 903640 mates make up the pairs; of these: 874528 (96.78%) aligned 0 times 18424 (2.04%) aligned exactly 1 time 10688 (1.18%) aligned >1 times 89.07% overall alignment rate Time searching: 00:03:06 Overall time: 00:03:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 345773 / 3546272 = 0.0975 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 08:58:47: # Command line: callpeak -t ERX1236191.bam -f BAM -g 12100000 -n ERX1236191.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1236191.05 # format = BAM # ChIP-seq file = ['ERX1236191.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:58:47: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:58:47: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:58:47: # Command line: callpeak -t ERX1236191.bam -f BAM -g 12100000 -n ERX1236191.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1236191.20 # format = BAM # ChIP-seq file = ['ERX1236191.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:58:47: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:58:47: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:58:47: # Command line: callpeak -t ERX1236191.bam -f BAM -g 12100000 -n ERX1236191.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1236191.10 # format = BAM # ChIP-seq file = ['ERX1236191.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:58:47: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:58:47: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:58:53: 1000000 INFO @ Fri, 15 Feb 2019 08:58:53: 1000000 INFO @ Fri, 15 Feb 2019 08:58:53: 1000000 INFO @ Fri, 15 Feb 2019 08:58:59: 2000000 INFO @ Fri, 15 Feb 2019 08:59:00: 2000000 INFO @ Fri, 15 Feb 2019 08:59:00: 2000000 INFO @ Fri, 15 Feb 2019 08:59:05: 3000000 INFO @ Fri, 15 Feb 2019 08:59:06: 3000000 INFO @ Fri, 15 Feb 2019 08:59:06: 3000000 INFO @ Fri, 15 Feb 2019 08:59:12: 4000000 INFO @ Fri, 15 Feb 2019 08:59:13: 4000000 INFO @ Fri, 15 Feb 2019 08:59:13: 4000000 INFO @ Fri, 15 Feb 2019 08:59:18: 5000000 INFO @ Fri, 15 Feb 2019 08:59:20: 5000000 INFO @ Fri, 15 Feb 2019 08:59:20: 5000000 INFO @ Fri, 15 Feb 2019 08:59:24: 6000000 INFO @ Fri, 15 Feb 2019 08:59:26: 6000000 INFO @ Fri, 15 Feb 2019 08:59:26: 6000000 INFO @ Fri, 15 Feb 2019 08:59:27: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:59:27: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:59:27: #1 total tags in treatment: 3180794 INFO @ Fri, 15 Feb 2019 08:59:27: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:59:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:59:27: #1 tags after filtering in treatment: 2321930 INFO @ Fri, 15 Feb 2019 08:59:27: #1 Redundant rate of treatment: 0.27 INFO @ Fri, 15 Feb 2019 08:59:27: #1 finished! INFO @ Fri, 15 Feb 2019 08:59:27: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:59:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:59:27: #2 number of paired peaks: 213 WARNING @ Fri, 15 Feb 2019 08:59:27: Fewer paired peaks (213) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 213 pairs to build model! INFO @ Fri, 15 Feb 2019 08:59:27: start model_add_line... INFO @ Fri, 15 Feb 2019 08:59:27: start X-correlation... INFO @ Fri, 15 Feb 2019 08:59:27: end of X-cor INFO @ Fri, 15 Feb 2019 08:59:27: #2 finished! INFO @ Fri, 15 Feb 2019 08:59:27: #2 predicted fragment length is 223 bps INFO @ Fri, 15 Feb 2019 08:59:27: #2 alternative fragment length(s) may be 2,223 bps INFO @ Fri, 15 Feb 2019 08:59:27: #2.2 Generate R script for model : ERX1236191.05_model.r INFO @ Fri, 15 Feb 2019 08:59:27: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:59:27: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:59:29: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:59:29: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:59:29: #1 total tags in treatment: 3180794 INFO @ Fri, 15 Feb 2019 08:59:29: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:59:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:59:29: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:59:29: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:59:29: #1 total tags in treatment: 3180794 INFO @ Fri, 15 Feb 2019 08:59:29: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:59:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:59:29: #1 tags after filtering in treatment: 2321930 INFO @ Fri, 15 Feb 2019 08:59:29: #1 Redundant rate of treatment: 0.27 INFO @ Fri, 15 Feb 2019 08:59:29: #1 finished! INFO @ Fri, 15 Feb 2019 08:59:29: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:59:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:59:29: #1 tags after filtering in treatment: 2321930 INFO @ Fri, 15 Feb 2019 08:59:29: #1 Redundant rate of treatment: 0.27 INFO @ Fri, 15 Feb 2019 08:59:29: #1 finished! INFO @ Fri, 15 Feb 2019 08:59:29: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:59:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:59:29: #2 number of paired peaks: 213 WARNING @ Fri, 15 Feb 2019 08:59:29: Fewer paired peaks (213) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 213 pairs to build model! INFO @ Fri, 15 Feb 2019 08:59:29: start model_add_line... INFO @ Fri, 15 Feb 2019 08:59:30: #2 number of paired peaks: 213 WARNING @ Fri, 15 Feb 2019 08:59:30: Fewer paired peaks (213) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 213 pairs to build model! INFO @ Fri, 15 Feb 2019 08:59:30: start model_add_line... INFO @ Fri, 15 Feb 2019 08:59:30: start X-correlation... INFO @ Fri, 15 Feb 2019 08:59:30: end of X-cor INFO @ Fri, 15 Feb 2019 08:59:30: #2 finished! INFO @ Fri, 15 Feb 2019 08:59:30: #2 predicted fragment length is 223 bps INFO @ Fri, 15 Feb 2019 08:59:30: #2 alternative fragment length(s) may be 2,223 bps INFO @ Fri, 15 Feb 2019 08:59:30: #2.2 Generate R script for model : ERX1236191.20_model.r INFO @ Fri, 15 Feb 2019 08:59:30: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:59:30: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:59:30: start X-correlation... INFO @ Fri, 15 Feb 2019 08:59:30: end of X-cor INFO @ Fri, 15 Feb 2019 08:59:30: #2 finished! INFO @ Fri, 15 Feb 2019 08:59:30: #2 predicted fragment length is 223 bps INFO @ Fri, 15 Feb 2019 08:59:30: #2 alternative fragment length(s) may be 2,223 bps INFO @ Fri, 15 Feb 2019 08:59:30: #2.2 Generate R script for model : ERX1236191.10_model.r INFO @ Fri, 15 Feb 2019 08:59:30: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:59:30: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:59:35: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:59:37: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:59:38: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:59:38: #4 Write output xls file... ERX1236191.05_peaks.xls INFO @ Fri, 15 Feb 2019 08:59:38: #4 Write peak in narrowPeak format file... ERX1236191.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 08:59:38: #4 Write summits bed file... ERX1236191.05_summits.bed INFO @ Fri, 15 Feb 2019 08:59:38: Done! pass1 - making usageList (17 chroms): 2 millis pass2 - checking and writing primary data (513 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 08:59:40: #4 Write output xls file... ERX1236191.20_peaks.xls INFO @ Fri, 15 Feb 2019 08:59:40: #4 Write peak in narrowPeak format file... ERX1236191.20_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 08:59:40: #4 Write summits bed file... ERX1236191.20_summits.bed INFO @ Fri, 15 Feb 2019 08:59:40: Done! pass1 - making usageList (17 chroms): 2 millis pass2 - checking and writing primary data (151 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 08:59:40: #4 Write output xls file... ERX1236191.10_peaks.xls INFO @ Fri, 15 Feb 2019 08:59:40: #4 Write peak in narrowPeak format file... ERX1236191.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 08:59:40: #4 Write summits bed file... ERX1236191.10_summits.bed INFO @ Fri, 15 Feb 2019 08:59:40: Done! pass1 - making usageList (17 chroms): 2 millis pass2 - checking and writing primary data (286 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。