Job ID = 11633380 sra ファイルのダウンロード中... Completed: 45557K bytes transferred in 3 seconds (98822K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 741855 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236158/ERR1162751.sra Written 741855 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1236158/ERR1162751.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:34 741855 reads; of these: 741855 (100.00%) were paired; of these: 73877 (9.96%) aligned concordantly 0 times 602018 (81.15%) aligned concordantly exactly 1 time 65960 (8.89%) aligned concordantly >1 times ---- 73877 pairs aligned concordantly 0 times; of these: 6000 (8.12%) aligned discordantly 1 time ---- 67877 pairs aligned 0 times concordantly or discordantly; of these: 135754 mates make up the pairs; of these: 131370 (96.77%) aligned 0 times 2450 (1.80%) aligned exactly 1 time 1934 (1.42%) aligned >1 times 91.15% overall alignment rate Time searching: 00:00:34 Overall time: 00:00:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 13715 / 673850 = 0.0204 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 08:50:53: # Command line: callpeak -t ERX1236158.bam -f BAM -g 12100000 -n ERX1236158.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1236158.05 # format = BAM # ChIP-seq file = ['ERX1236158.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:50:53: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:50:53: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:50:53: # Command line: callpeak -t ERX1236158.bam -f BAM -g 12100000 -n ERX1236158.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1236158.20 # format = BAM # ChIP-seq file = ['ERX1236158.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:50:53: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:50:53: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:50:53: # Command line: callpeak -t ERX1236158.bam -f BAM -g 12100000 -n ERX1236158.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1236158.10 # format = BAM # ChIP-seq file = ['ERX1236158.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:50:53: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:50:53: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:50:59: 1000000 INFO @ Fri, 15 Feb 2019 08:50:59: 1000000 INFO @ Fri, 15 Feb 2019 08:51:00: 1000000 INFO @ Fri, 15 Feb 2019 08:51:01: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:51:01: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:51:01: #1 total tags in treatment: 654366 INFO @ Fri, 15 Feb 2019 08:51:01: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:51:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:51:01: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:51:01: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:51:01: #1 total tags in treatment: 654366 INFO @ Fri, 15 Feb 2019 08:51:01: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:51:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:51:01: #1 tags after filtering in treatment: 634099 INFO @ Fri, 15 Feb 2019 08:51:01: #1 Redundant rate of treatment: 0.03 INFO @ Fri, 15 Feb 2019 08:51:01: #1 finished! INFO @ Fri, 15 Feb 2019 08:51:01: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:51:01: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:51:01: #1 tags after filtering in treatment: 634099 INFO @ Fri, 15 Feb 2019 08:51:01: #1 Redundant rate of treatment: 0.03 INFO @ Fri, 15 Feb 2019 08:51:01: #1 finished! INFO @ Fri, 15 Feb 2019 08:51:01: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:51:01: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:51:01: #2 number of paired peaks: 123 WARNING @ Fri, 15 Feb 2019 08:51:01: Fewer paired peaks (123) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 123 pairs to build model! INFO @ Fri, 15 Feb 2019 08:51:01: start model_add_line... INFO @ Fri, 15 Feb 2019 08:51:01: #2 number of paired peaks: 123 WARNING @ Fri, 15 Feb 2019 08:51:01: Fewer paired peaks (123) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 123 pairs to build model! INFO @ Fri, 15 Feb 2019 08:51:01: start model_add_line... INFO @ Fri, 15 Feb 2019 08:51:01: start X-correlation... INFO @ Fri, 15 Feb 2019 08:51:01: start X-correlation... INFO @ Fri, 15 Feb 2019 08:51:01: end of X-cor INFO @ Fri, 15 Feb 2019 08:51:01: #2 finished! INFO @ Fri, 15 Feb 2019 08:51:01: #2 predicted fragment length is 263 bps INFO @ Fri, 15 Feb 2019 08:51:01: #2 alternative fragment length(s) may be 4,32,53,93,134,159,201,224,263,312,352,412,542 bps INFO @ Fri, 15 Feb 2019 08:51:01: #2.2 Generate R script for model : ERX1236158.20_model.r INFO @ Fri, 15 Feb 2019 08:51:01: end of X-cor INFO @ Fri, 15 Feb 2019 08:51:01: #2 finished! INFO @ Fri, 15 Feb 2019 08:51:01: #2 predicted fragment length is 263 bps INFO @ Fri, 15 Feb 2019 08:51:01: #2 alternative fragment length(s) may be 4,32,53,93,134,159,201,224,263,312,352,412,542 bps INFO @ Fri, 15 Feb 2019 08:51:01: #2.2 Generate R script for model : ERX1236158.10_model.r INFO @ Fri, 15 Feb 2019 08:51:01: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:51:01: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:51:01: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:51:01: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:51:02: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:51:02: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:51:02: #1 total tags in treatment: 654366 INFO @ Fri, 15 Feb 2019 08:51:02: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:51:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:51:02: #1 tags after filtering in treatment: 634099 INFO @ Fri, 15 Feb 2019 08:51:02: #1 Redundant rate of treatment: 0.03 INFO @ Fri, 15 Feb 2019 08:51:02: #1 finished! INFO @ Fri, 15 Feb 2019 08:51:02: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:51:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:51:02: #2 number of paired peaks: 123 WARNING @ Fri, 15 Feb 2019 08:51:02: Fewer paired peaks (123) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 123 pairs to build model! INFO @ Fri, 15 Feb 2019 08:51:02: start model_add_line... INFO @ Fri, 15 Feb 2019 08:51:02: start X-correlation... INFO @ Fri, 15 Feb 2019 08:51:02: end of X-cor INFO @ Fri, 15 Feb 2019 08:51:02: #2 finished! INFO @ Fri, 15 Feb 2019 08:51:02: #2 predicted fragment length is 263 bps INFO @ Fri, 15 Feb 2019 08:51:02: #2 alternative fragment length(s) may be 4,32,53,93,134,159,201,224,263,312,352,412,542 bps INFO @ Fri, 15 Feb 2019 08:51:02: #2.2 Generate R script for model : ERX1236158.05_model.r INFO @ Fri, 15 Feb 2019 08:51:02: #3 Call peaks... INFO @ Fri, 15 Feb 2019 08:51:02: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 08:51:03: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:51:03: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:51:04: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 08:51:04: #4 Write output xls file... ERX1236158.20_peaks.xls INFO @ Fri, 15 Feb 2019 08:51:04: #4 Write peak in narrowPeak format file... ERX1236158.20_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 08:51:04: #4 Write summits bed file... ERX1236158.20_summits.bed INFO @ Fri, 15 Feb 2019 08:51:04: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (49 records, 4 fields): 4 millis INFO @ Fri, 15 Feb 2019 08:51:04: #4 Write output xls file... ERX1236158.10_peaks.xls INFO @ Fri, 15 Feb 2019 08:51:04: #4 Write peak in narrowPeak format file... ERX1236158.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 08:51:04: #4 Write summits bed file... ERX1236158.10_summits.bed INFO @ Fri, 15 Feb 2019 08:51:04: Done! CompletedMACS2peakCalling pass1 - making usageList (16 chroms): 2 millis pass2 - checking and writing primary data (146 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 08:51:05: #4 Write output xls file... ERX1236158.05_peaks.xls INFO @ Fri, 15 Feb 2019 08:51:05: #4 Write peak in narrowPeak format file... ERX1236158.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 08:51:05: #4 Write summits bed file... ERX1236158.05_summits.bed INFO @ Fri, 15 Feb 2019 08:51:05: Done! pass1 - making usageList (17 chroms): 4 millis pass2 - checking and writing primary data (257 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。