Job ID = 11633219 sra ファイルのダウンロード中... Completed: 201991K bytes transferred in 6 seconds (275132K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 2859543 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1174155/ERR1094669.sra Written 2859543 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1174155/ERR1094669.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:59 2859543 reads; of these: 2859543 (100.00%) were paired; of these: 300579 (10.51%) aligned concordantly 0 times 2344670 (81.99%) aligned concordantly exactly 1 time 214294 (7.49%) aligned concordantly >1 times ---- 300579 pairs aligned concordantly 0 times; of these: 45698 (15.20%) aligned discordantly 1 time ---- 254881 pairs aligned 0 times concordantly or discordantly; of these: 509762 mates make up the pairs; of these: 432191 (84.78%) aligned 0 times 60939 (11.95%) aligned exactly 1 time 16632 (3.26%) aligned >1 times 92.44% overall alignment rate Time searching: 00:01:59 Overall time: 00:01:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 57228 / 2603414 = 0.0220 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 08:33:44: # Command line: callpeak -t ERX1174155.bam -f BAM -g 12100000 -n ERX1174155.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1174155.20 # format = BAM # ChIP-seq file = ['ERX1174155.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:33:44: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:33:44: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:33:45: # Command line: callpeak -t ERX1174155.bam -f BAM -g 12100000 -n ERX1174155.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1174155.05 # format = BAM # ChIP-seq file = ['ERX1174155.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:33:45: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:33:45: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:33:45: # Command line: callpeak -t ERX1174155.bam -f BAM -g 12100000 -n ERX1174155.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1174155.10 # format = BAM # ChIP-seq file = ['ERX1174155.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:33:45: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:33:45: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:33:51: 1000000 INFO @ Fri, 15 Feb 2019 08:33:51: 1000000 INFO @ Fri, 15 Feb 2019 08:33:51: 1000000 INFO @ Fri, 15 Feb 2019 08:33:57: 2000000 INFO @ Fri, 15 Feb 2019 08:33:58: 2000000 INFO @ Fri, 15 Feb 2019 08:33:58: 2000000 INFO @ Fri, 15 Feb 2019 08:34:04: 3000000 INFO @ Fri, 15 Feb 2019 08:34:05: 3000000 INFO @ Fri, 15 Feb 2019 08:34:05: 3000000 INFO @ Fri, 15 Feb 2019 08:34:11: 4000000 INFO @ Fri, 15 Feb 2019 08:34:11: 4000000 INFO @ Fri, 15 Feb 2019 08:34:11: 4000000 INFO @ Fri, 15 Feb 2019 08:34:17: 5000000 INFO @ Fri, 15 Feb 2019 08:34:18: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:34:18: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:34:18: #1 total tags in treatment: 2502307 INFO @ Fri, 15 Feb 2019 08:34:18: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:34:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:34:18: #1 tags after filtering in treatment: 2288310 INFO @ Fri, 15 Feb 2019 08:34:18: #1 Redundant rate of treatment: 0.09 INFO @ Fri, 15 Feb 2019 08:34:18: #1 finished! INFO @ Fri, 15 Feb 2019 08:34:18: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:34:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:34:18: 5000000 INFO @ Fri, 15 Feb 2019 08:34:18: 5000000 INFO @ Fri, 15 Feb 2019 08:34:18: #2 number of paired peaks: 35 WARNING @ Fri, 15 Feb 2019 08:34:18: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 08:34:18: Process for pairing-model is terminated! cat: ERX1174155.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1174155.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1174155.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1174155.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 08:34:19: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:34:19: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:34:19: #1 total tags in treatment: 2502307 INFO @ Fri, 15 Feb 2019 08:34:19: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:34:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:34:19: #1 tags after filtering in treatment: 2288310 INFO @ Fri, 15 Feb 2019 08:34:19: #1 Redundant rate of treatment: 0.09 INFO @ Fri, 15 Feb 2019 08:34:19: #1 finished! INFO @ Fri, 15 Feb 2019 08:34:19: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:34:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:34:19: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:34:19: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:34:19: #1 total tags in treatment: 2502307 INFO @ Fri, 15 Feb 2019 08:34:19: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:34:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:34:19: #1 tags after filtering in treatment: 2288310 INFO @ Fri, 15 Feb 2019 08:34:19: #1 Redundant rate of treatment: 0.09 INFO @ Fri, 15 Feb 2019 08:34:19: #1 finished! INFO @ Fri, 15 Feb 2019 08:34:19: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:34:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:34:19: #2 number of paired peaks: 35 WARNING @ Fri, 15 Feb 2019 08:34:19: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 08:34:19: Process for pairing-model is terminated! cat: ERX1174155.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1174155.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1174155.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1174155.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 08:34:20: #2 number of paired peaks: 35 WARNING @ Fri, 15 Feb 2019 08:34:20: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 08:34:20: Process for pairing-model is terminated! cat: ERX1174155.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1174155.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1174155.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1174155.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。