Job ID = 11633100 sra ファイルのダウンロード中... Completed: 241873K bytes transferred in 6 seconds (314120K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1174038/ERR1094552.sra Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1174038/ERR1094552.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:06 4000000 reads; of these: 4000000 (100.00%) were paired; of these: 391423 (9.79%) aligned concordantly 0 times 3352894 (83.82%) aligned concordantly exactly 1 time 255683 (6.39%) aligned concordantly >1 times ---- 391423 pairs aligned concordantly 0 times; of these: 109800 (28.05%) aligned discordantly 1 time ---- 281623 pairs aligned 0 times concordantly or discordantly; of these: 563246 mates make up the pairs; of these: 531177 (94.31%) aligned 0 times 14079 (2.50%) aligned exactly 1 time 17990 (3.19%) aligned >1 times 93.36% overall alignment rate Time searching: 00:03:06 Overall time: 00:03:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 17938 / 3711850 = 0.0048 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 08:39:15: # Command line: callpeak -t ERX1174038.bam -f BAM -g 12100000 -n ERX1174038.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1174038.20 # format = BAM # ChIP-seq file = ['ERX1174038.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:39:15: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:39:15: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:39:15: # Command line: callpeak -t ERX1174038.bam -f BAM -g 12100000 -n ERX1174038.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1174038.05 # format = BAM # ChIP-seq file = ['ERX1174038.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:39:15: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:39:15: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:39:15: # Command line: callpeak -t ERX1174038.bam -f BAM -g 12100000 -n ERX1174038.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1174038.10 # format = BAM # ChIP-seq file = ['ERX1174038.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:39:15: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:39:15: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:39:21: 1000000 INFO @ Fri, 15 Feb 2019 08:39:22: 1000000 INFO @ Fri, 15 Feb 2019 08:39:22: 1000000 INFO @ Fri, 15 Feb 2019 08:39:28: 2000000 INFO @ Fri, 15 Feb 2019 08:39:29: 2000000 INFO @ Fri, 15 Feb 2019 08:39:29: 2000000 INFO @ Fri, 15 Feb 2019 08:39:35: 3000000 INFO @ Fri, 15 Feb 2019 08:39:36: 3000000 INFO @ Fri, 15 Feb 2019 08:39:36: 3000000 INFO @ Fri, 15 Feb 2019 08:39:41: 4000000 INFO @ Fri, 15 Feb 2019 08:39:43: 4000000 INFO @ Fri, 15 Feb 2019 08:39:43: 4000000 INFO @ Fri, 15 Feb 2019 08:39:48: 5000000 INFO @ Fri, 15 Feb 2019 08:39:50: 5000000 INFO @ Fri, 15 Feb 2019 08:39:50: 5000000 INFO @ Fri, 15 Feb 2019 08:39:55: 6000000 INFO @ Fri, 15 Feb 2019 08:39:57: 6000000 INFO @ Fri, 15 Feb 2019 08:39:57: 6000000 INFO @ Fri, 15 Feb 2019 08:40:01: 7000000 INFO @ Fri, 15 Feb 2019 08:40:04: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:40:04: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:40:04: #1 total tags in treatment: 3591046 INFO @ Fri, 15 Feb 2019 08:40:04: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:40:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:40:04: 7000000 INFO @ Fri, 15 Feb 2019 08:40:04: #1 tags after filtering in treatment: 3254289 INFO @ Fri, 15 Feb 2019 08:40:04: #1 Redundant rate of treatment: 0.09 INFO @ Fri, 15 Feb 2019 08:40:04: #1 finished! INFO @ Fri, 15 Feb 2019 08:40:04: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:40:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:40:04: 7000000 INFO @ Fri, 15 Feb 2019 08:40:04: #2 number of paired peaks: 4 WARNING @ Fri, 15 Feb 2019 08:40:04: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 08:40:04: Process for pairing-model is terminated! cat: ERX1174038.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1174038.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1174038.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1174038.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 08:40:07: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:40:07: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:40:07: #1 total tags in treatment: 3591046 INFO @ Fri, 15 Feb 2019 08:40:07: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:40:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:40:07: #1 tags after filtering in treatment: 3254289 INFO @ Fri, 15 Feb 2019 08:40:07: #1 Redundant rate of treatment: 0.09 INFO @ Fri, 15 Feb 2019 08:40:07: #1 finished! INFO @ Fri, 15 Feb 2019 08:40:07: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:40:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:40:07: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:40:07: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:40:07: #1 total tags in treatment: 3591046 INFO @ Fri, 15 Feb 2019 08:40:07: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:40:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:40:07: #1 tags after filtering in treatment: 3254289 INFO @ Fri, 15 Feb 2019 08:40:07: #1 Redundant rate of treatment: 0.09 INFO @ Fri, 15 Feb 2019 08:40:07: #1 finished! INFO @ Fri, 15 Feb 2019 08:40:07: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:40:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:40:07: #2 number of paired peaks: 4 WARNING @ Fri, 15 Feb 2019 08:40:07: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 08:40:07: Process for pairing-model is terminated! cat: ERX1174038.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1174038.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1174038.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1174038.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 08:40:08: #2 number of paired peaks: 4 WARNING @ Fri, 15 Feb 2019 08:40:08: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 08:40:08: Process for pairing-model is terminated! cat: ERX1174038.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1174038.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1174038.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1174038.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。