Job ID = 11633023 sra ファイルのダウンロード中... Completed: 268721K bytes transferred in 7 seconds (309261K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1173961/ERR1094475.sra Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1173961/ERR1094475.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:53 4000000 reads; of these: 4000000 (100.00%) were paired; of these: 452159 (11.30%) aligned concordantly 0 times 3229085 (80.73%) aligned concordantly exactly 1 time 318756 (7.97%) aligned concordantly >1 times ---- 452159 pairs aligned concordantly 0 times; of these: 26253 (5.81%) aligned discordantly 1 time ---- 425906 pairs aligned 0 times concordantly or discordantly; of these: 851812 mates make up the pairs; of these: 833674 (97.87%) aligned 0 times 11080 (1.30%) aligned exactly 1 time 7058 (0.83%) aligned >1 times 89.58% overall alignment rate Time searching: 00:02:53 Overall time: 00:02:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 44745 / 3563760 = 0.0126 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 08:13:31: # Command line: callpeak -t ERX1173961.bam -f BAM -g 12100000 -n ERX1173961.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1173961.20 # format = BAM # ChIP-seq file = ['ERX1173961.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:13:31: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:13:31: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:13:31: # Command line: callpeak -t ERX1173961.bam -f BAM -g 12100000 -n ERX1173961.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1173961.10 # format = BAM # ChIP-seq file = ['ERX1173961.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:13:31: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:13:31: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:13:31: # Command line: callpeak -t ERX1173961.bam -f BAM -g 12100000 -n ERX1173961.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1173961.05 # format = BAM # ChIP-seq file = ['ERX1173961.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 08:13:31: #1 read tag files... INFO @ Fri, 15 Feb 2019 08:13:31: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 08:13:36: 1000000 INFO @ Fri, 15 Feb 2019 08:13:37: 1000000 INFO @ Fri, 15 Feb 2019 08:13:37: 1000000 INFO @ Fri, 15 Feb 2019 08:13:42: 2000000 INFO @ Fri, 15 Feb 2019 08:13:43: 2000000 INFO @ Fri, 15 Feb 2019 08:13:43: 2000000 INFO @ Fri, 15 Feb 2019 08:13:48: 3000000 INFO @ Fri, 15 Feb 2019 08:13:49: 3000000 INFO @ Fri, 15 Feb 2019 08:13:49: 3000000 INFO @ Fri, 15 Feb 2019 08:13:53: 4000000 INFO @ Fri, 15 Feb 2019 08:13:54: 4000000 INFO @ Fri, 15 Feb 2019 08:13:55: 4000000 INFO @ Fri, 15 Feb 2019 08:13:59: 5000000 INFO @ Fri, 15 Feb 2019 08:14:01: 5000000 INFO @ Fri, 15 Feb 2019 08:14:01: 5000000 INFO @ Fri, 15 Feb 2019 08:14:05: 6000000 INFO @ Fri, 15 Feb 2019 08:14:07: 6000000 INFO @ Fri, 15 Feb 2019 08:14:07: 6000000 INFO @ Fri, 15 Feb 2019 08:14:10: 7000000 INFO @ Fri, 15 Feb 2019 08:14:11: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:14:11: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:14:11: #1 total tags in treatment: 3503196 INFO @ Fri, 15 Feb 2019 08:14:11: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:14:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:14:11: #1 tags after filtering in treatment: 3179906 INFO @ Fri, 15 Feb 2019 08:14:11: #1 Redundant rate of treatment: 0.09 INFO @ Fri, 15 Feb 2019 08:14:11: #1 finished! INFO @ Fri, 15 Feb 2019 08:14:11: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:14:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:14:11: #2 number of paired peaks: 29 WARNING @ Fri, 15 Feb 2019 08:14:11: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 08:14:11: Process for pairing-model is terminated! cat: ERX1173961.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 5 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1173961.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1173961.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1173961.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 08:14:13: 7000000 INFO @ Fri, 15 Feb 2019 08:14:13: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:14:13: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:14:13: #1 total tags in treatment: 3503196 INFO @ Fri, 15 Feb 2019 08:14:13: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:14:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:14:14: #1 tags after filtering in treatment: 3179906 INFO @ Fri, 15 Feb 2019 08:14:14: #1 Redundant rate of treatment: 0.09 INFO @ Fri, 15 Feb 2019 08:14:14: #1 finished! INFO @ Fri, 15 Feb 2019 08:14:14: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:14:14: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:14:14: 7000000 INFO @ Fri, 15 Feb 2019 08:14:14: #2 number of paired peaks: 29 WARNING @ Fri, 15 Feb 2019 08:14:14: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 08:14:14: Process for pairing-model is terminated! cat: ERX1173961.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 7 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1173961.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1173961.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1173961.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 08:14:14: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 08:14:14: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 08:14:14: #1 total tags in treatment: 3503196 INFO @ Fri, 15 Feb 2019 08:14:14: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 08:14:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 08:14:14: #1 tags after filtering in treatment: 3179906 INFO @ Fri, 15 Feb 2019 08:14:14: #1 Redundant rate of treatment: 0.09 INFO @ Fri, 15 Feb 2019 08:14:14: #1 finished! INFO @ Fri, 15 Feb 2019 08:14:14: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 08:14:14: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 08:14:14: #2 number of paired peaks: 29 WARNING @ Fri, 15 Feb 2019 08:14:14: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 08:14:14: Process for pairing-model is terminated! cat: ERX1173961.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1173961.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1173961.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1173961.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。