Job ID = 2003989 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 3,499,667 reads read : 3,499,667 reads written : 3,499,667 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:41 3499667 reads; of these: 3499667 (100.00%) were unpaired; of these: 787414 (22.50%) aligned 0 times 1819970 (52.00%) aligned exactly 1 time 892283 (25.50%) aligned >1 times 77.50% overall alignment rate Time searching: 00:00:41 Overall time: 00:00:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1845061 / 2712253 = 0.6803 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 14:22:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 14:22:15: #1 read tag files... INFO @ Fri, 05 Jul 2019 14:22:15: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 14:22:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 14:22:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 14:22:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 14:22:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 14:22:17: #1 read tag files... INFO @ Fri, 05 Jul 2019 14:22:17: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 14:22:23: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 14:22:23: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 14:22:23: #1 total tags in treatment: 867192 INFO @ Fri, 05 Jul 2019 14:22:23: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 14:22:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 14:22:23: #1 tags after filtering in treatment: 867192 INFO @ Fri, 05 Jul 2019 14:22:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 14:22:23: #1 finished! INFO @ Fri, 05 Jul 2019 14:22:23: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 14:22:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 14:22:24: #2 number of paired peaks: 702 WARNING @ Fri, 05 Jul 2019 14:22:24: Fewer paired peaks (702) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 702 pairs to build model! INFO @ Fri, 05 Jul 2019 14:22:24: start model_add_line... INFO @ Fri, 05 Jul 2019 14:22:24: start X-correlation... INFO @ Fri, 05 Jul 2019 14:22:24: end of X-cor INFO @ Fri, 05 Jul 2019 14:22:24: #2 finished! INFO @ Fri, 05 Jul 2019 14:22:24: #2 predicted fragment length is 317 bps INFO @ Fri, 05 Jul 2019 14:22:24: #2 alternative fragment length(s) may be 317 bps INFO @ Fri, 05 Jul 2019 14:22:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.05_model.r INFO @ Fri, 05 Jul 2019 14:22:24: #3 Call peaks... INFO @ Fri, 05 Jul 2019 14:22:24: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 14:22:24: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 14:22:24: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 14:22:24: #1 total tags in treatment: 867192 INFO @ Fri, 05 Jul 2019 14:22:24: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 14:22:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 14:22:24: #1 tags after filtering in treatment: 867192 INFO @ Fri, 05 Jul 2019 14:22:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 14:22:24: #1 finished! INFO @ Fri, 05 Jul 2019 14:22:24: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 14:22:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 14:22:25: #2 number of paired peaks: 702 WARNING @ Fri, 05 Jul 2019 14:22:25: Fewer paired peaks (702) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 702 pairs to build model! INFO @ Fri, 05 Jul 2019 14:22:25: start model_add_line... INFO @ Fri, 05 Jul 2019 14:22:25: start X-correlation... INFO @ Fri, 05 Jul 2019 14:22:25: end of X-cor INFO @ Fri, 05 Jul 2019 14:22:25: #2 finished! INFO @ Fri, 05 Jul 2019 14:22:25: #2 predicted fragment length is 317 bps INFO @ Fri, 05 Jul 2019 14:22:25: #2 alternative fragment length(s) may be 317 bps INFO @ Fri, 05 Jul 2019 14:22:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.10_model.r INFO @ Fri, 05 Jul 2019 14:22:25: #3 Call peaks... INFO @ Fri, 05 Jul 2019 14:22:25: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 14:22:25: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 14:22:25: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 14:22:25: #1 total tags in treatment: 867192 INFO @ Fri, 05 Jul 2019 14:22:25: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 14:22:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 14:22:25: #1 tags after filtering in treatment: 867192 INFO @ Fri, 05 Jul 2019 14:22:25: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 14:22:25: #1 finished! INFO @ Fri, 05 Jul 2019 14:22:25: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 14:22:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 14:22:25: #2 number of paired peaks: 702 WARNING @ Fri, 05 Jul 2019 14:22:25: Fewer paired peaks (702) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 702 pairs to build model! INFO @ Fri, 05 Jul 2019 14:22:25: start model_add_line... INFO @ Fri, 05 Jul 2019 14:22:25: start X-correlation... INFO @ Fri, 05 Jul 2019 14:22:25: end of X-cor INFO @ Fri, 05 Jul 2019 14:22:25: #2 finished! INFO @ Fri, 05 Jul 2019 14:22:25: #2 predicted fragment length is 317 bps INFO @ Fri, 05 Jul 2019 14:22:25: #2 alternative fragment length(s) may be 317 bps INFO @ Fri, 05 Jul 2019 14:22:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.20_model.r INFO @ Fri, 05 Jul 2019 14:22:25: #3 Call peaks... INFO @ Fri, 05 Jul 2019 14:22:25: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 14:22:29: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 14:22:30: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 14:22:30: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 14:22:30: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.05_peaks.xls INFO @ Fri, 05 Jul 2019 14:22:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.05_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 14:22:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.05_summits.bed INFO @ Fri, 05 Jul 2019 14:22:30: Done! pass1 - making usageList (17 chroms): 2 millis pass2 - checking and writing primary data (1588 records, 4 fields): 7 millis INFO @ Fri, 05 Jul 2019 14:22:31: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.10_peaks.xls INFO @ Fri, 05 Jul 2019 14:22:32: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.20_peaks.xls INFO @ Fri, 05 Jul 2019 14:22:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.20_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 14:22:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.20_summits.bed INFO @ Fri, 05 Jul 2019 14:22:32: Done! INFO @ Fri, 05 Jul 2019 14:22:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.10_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 14:22:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX085705/ERX085705.10_summits.bed pass1 - making usageList (16 chroms): 2 millis INFO @ Fri, 05 Jul 2019 14:22:33: Done! pass2 - checking and writing primary data (808 records, 4 fields): 6 millis pass1 - making usageList (17 chroms): 2 millis pass2 - checking and writing primary data (1199 records, 4 fields): 6 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... CompletedMACS2peakCalling CompletedMACS2peakCalling BigWig に変換しました。