Job ID = 2003830 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 1,304,306 reads read : 1,304,306 reads written : 1,304,306 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:14 1304306 reads; of these: 1304306 (100.00%) were unpaired; of these: 442882 (33.96%) aligned 0 times 741218 (56.83%) aligned exactly 1 time 120206 (9.22%) aligned >1 times 66.04% overall alignment rate Time searching: 00:00:14 Overall time: 00:00:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 483050 / 861424 = 0.5608 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 14:16:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 14:16:30: #1 read tag files... INFO @ Fri, 05 Jul 2019 14:16:30: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 14:16:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 14:16:31: #1 read tag files... INFO @ Fri, 05 Jul 2019 14:16:31: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 14:16:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 14:16:32: #1 read tag files... INFO @ Fri, 05 Jul 2019 14:16:32: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 14:16:33: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 14:16:33: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 14:16:33: #1 total tags in treatment: 378374 INFO @ Fri, 05 Jul 2019 14:16:33: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 14:16:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 14:16:33: #1 tags after filtering in treatment: 378374 INFO @ Fri, 05 Jul 2019 14:16:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 14:16:33: #1 finished! INFO @ Fri, 05 Jul 2019 14:16:33: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 14:16:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 14:16:33: #2 number of paired peaks: 378 WARNING @ Fri, 05 Jul 2019 14:16:33: Fewer paired peaks (378) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 378 pairs to build model! INFO @ Fri, 05 Jul 2019 14:16:33: start model_add_line... INFO @ Fri, 05 Jul 2019 14:16:33: start X-correlation... INFO @ Fri, 05 Jul 2019 14:16:33: end of X-cor INFO @ Fri, 05 Jul 2019 14:16:33: #2 finished! INFO @ Fri, 05 Jul 2019 14:16:33: #2 predicted fragment length is 141 bps INFO @ Fri, 05 Jul 2019 14:16:33: #2 alternative fragment length(s) may be 141 bps INFO @ Fri, 05 Jul 2019 14:16:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.05_model.r INFO @ Fri, 05 Jul 2019 14:16:33: #3 Call peaks... INFO @ Fri, 05 Jul 2019 14:16:33: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 14:16:35: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 14:16:35: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 14:16:35: #1 total tags in treatment: 378374 INFO @ Fri, 05 Jul 2019 14:16:35: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 14:16:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 14:16:35: #1 tags after filtering in treatment: 378374 INFO @ Fri, 05 Jul 2019 14:16:35: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 14:16:35: #1 finished! INFO @ Fri, 05 Jul 2019 14:16:35: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 14:16:35: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 14:16:35: #2 number of paired peaks: 378 WARNING @ Fri, 05 Jul 2019 14:16:35: Fewer paired peaks (378) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 378 pairs to build model! INFO @ Fri, 05 Jul 2019 14:16:35: start model_add_line... INFO @ Fri, 05 Jul 2019 14:16:35: start X-correlation... INFO @ Fri, 05 Jul 2019 14:16:35: end of X-cor INFO @ Fri, 05 Jul 2019 14:16:35: #2 finished! INFO @ Fri, 05 Jul 2019 14:16:35: #2 predicted fragment length is 141 bps INFO @ Fri, 05 Jul 2019 14:16:35: #2 alternative fragment length(s) may be 141 bps INFO @ Fri, 05 Jul 2019 14:16:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.10_model.r INFO @ Fri, 05 Jul 2019 14:16:35: #3 Call peaks... INFO @ Fri, 05 Jul 2019 14:16:35: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 14:16:35: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 14:16:35: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 14:16:35: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 14:16:35: #1 total tags in treatment: 378374 INFO @ Fri, 05 Jul 2019 14:16:35: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 14:16:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 14:16:35: #1 tags after filtering in treatment: 378374 INFO @ Fri, 05 Jul 2019 14:16:35: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 14:16:35: #1 finished! INFO @ Fri, 05 Jul 2019 14:16:35: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 14:16:35: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 14:16:35: #2 number of paired peaks: 378 WARNING @ Fri, 05 Jul 2019 14:16:35: Fewer paired peaks (378) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 378 pairs to build model! INFO @ Fri, 05 Jul 2019 14:16:35: start model_add_line... INFO @ Fri, 05 Jul 2019 14:16:35: start X-correlation... INFO @ Fri, 05 Jul 2019 14:16:35: end of X-cor INFO @ Fri, 05 Jul 2019 14:16:35: #2 finished! INFO @ Fri, 05 Jul 2019 14:16:35: #2 predicted fragment length is 141 bps INFO @ Fri, 05 Jul 2019 14:16:35: #2 alternative fragment length(s) may be 141 bps INFO @ Fri, 05 Jul 2019 14:16:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.20_model.r INFO @ Fri, 05 Jul 2019 14:16:35: #3 Call peaks... INFO @ Fri, 05 Jul 2019 14:16:35: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 14:16:35: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.05_peaks.xls INFO @ Fri, 05 Jul 2019 14:16:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.05_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 14:16:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.05_summits.bed INFO @ Fri, 05 Jul 2019 14:16:35: Done! pass1 - making usageList (16 chroms): 2 millis pass2 - checking and writing primary data (1135 records, 4 fields): 6 millis INFO @ Fri, 05 Jul 2019 14:16:36: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 14:16:37: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 14:16:37: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.10_peaks.xls INFO @ Fri, 05 Jul 2019 14:16:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.10_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 14:16:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.10_summits.bed INFO @ Fri, 05 Jul 2019 14:16:37: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (577 records, 4 fields): 4 millis INFO @ Fri, 05 Jul 2019 14:16:37: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.20_peaks.xls INFO @ Fri, 05 Jul 2019 14:16:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.20_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 14:16:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX008719/ERX008719.20_summits.bed INFO @ Fri, 05 Jul 2019 14:16:37: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (302 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... CompletedMACS2peakCalling BigWig に変換しました。 CompletedMACS2peakCalling