Job ID = 14171371 SRX = SRX9986162 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 22070623 spots for SRR13591517/SRR13591517.sra Written 22070623 spots for SRR13591517/SRR13591517.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171855 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:30 22070623 reads; of these: 22070623 (100.00%) were unpaired; of these: 2511257 (11.38%) aligned 0 times 14037096 (63.60%) aligned exactly 1 time 5522270 (25.02%) aligned >1 times 88.62% overall alignment rate Time searching: 00:06:31 Overall time: 00:06:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4066641 / 19559366 = 0.2079 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:37:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:37:55: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:37:55: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:38:01: 1000000 INFO @ Sat, 11 Dec 2021 11:38:07: 2000000 INFO @ Sat, 11 Dec 2021 11:38:12: 3000000 INFO @ Sat, 11 Dec 2021 11:38:17: 4000000 INFO @ Sat, 11 Dec 2021 11:38:23: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:38:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:38:26: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:38:26: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:38:28: 6000000 INFO @ Sat, 11 Dec 2021 11:38:32: 1000000 INFO @ Sat, 11 Dec 2021 11:38:34: 7000000 INFO @ Sat, 11 Dec 2021 11:38:37: 2000000 INFO @ Sat, 11 Dec 2021 11:38:39: 8000000 INFO @ Sat, 11 Dec 2021 11:38:43: 3000000 INFO @ Sat, 11 Dec 2021 11:38:44: 9000000 INFO @ Sat, 11 Dec 2021 11:38:48: 4000000 INFO @ Sat, 11 Dec 2021 11:38:49: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:38:54: 5000000 INFO @ Sat, 11 Dec 2021 11:38:55: 11000000 INFO @ Sat, 11 Dec 2021 11:38:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:38:55: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:38:55: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:38:59: 6000000 INFO @ Sat, 11 Dec 2021 11:39:00: 12000000 INFO @ Sat, 11 Dec 2021 11:39:01: 1000000 INFO @ Sat, 11 Dec 2021 11:39:05: 13000000 INFO @ Sat, 11 Dec 2021 11:39:05: 7000000 INFO @ Sat, 11 Dec 2021 11:39:06: 2000000 INFO @ Sat, 11 Dec 2021 11:39:10: 14000000 INFO @ Sat, 11 Dec 2021 11:39:11: 8000000 INFO @ Sat, 11 Dec 2021 11:39:11: 3000000 INFO @ Sat, 11 Dec 2021 11:39:15: 15000000 INFO @ Sat, 11 Dec 2021 11:39:16: 9000000 INFO @ Sat, 11 Dec 2021 11:39:16: 4000000 INFO @ Sat, 11 Dec 2021 11:39:18: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:39:18: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:39:18: #1 total tags in treatment: 15492725 INFO @ Sat, 11 Dec 2021 11:39:18: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:39:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:39:18: #1 tags after filtering in treatment: 15492725 INFO @ Sat, 11 Dec 2021 11:39:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:39:18: #1 finished! INFO @ Sat, 11 Dec 2021 11:39:18: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:39:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:39:19: #2 number of paired peaks: 25 WARNING @ Sat, 11 Dec 2021 11:39:19: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:39:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:39:21: 10000000 INFO @ Sat, 11 Dec 2021 11:39:22: 5000000 INFO @ Sat, 11 Dec 2021 11:39:27: 11000000 INFO @ Sat, 11 Dec 2021 11:39:27: 6000000 INFO @ Sat, 11 Dec 2021 11:39:32: 12000000 INFO @ Sat, 11 Dec 2021 11:39:32: 7000000 INFO @ Sat, 11 Dec 2021 11:39:37: 13000000 INFO @ Sat, 11 Dec 2021 11:39:38: 8000000 INFO @ Sat, 11 Dec 2021 11:39:42: 14000000 INFO @ Sat, 11 Dec 2021 11:39:43: 9000000 INFO @ Sat, 11 Dec 2021 11:39:47: 15000000 INFO @ Sat, 11 Dec 2021 11:39:49: 10000000 INFO @ Sat, 11 Dec 2021 11:39:50: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:39:50: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:39:50: #1 total tags in treatment: 15492725 INFO @ Sat, 11 Dec 2021 11:39:50: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:39:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:39:50: #1 tags after filtering in treatment: 15492725 INFO @ Sat, 11 Dec 2021 11:39:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:39:50: #1 finished! INFO @ Sat, 11 Dec 2021 11:39:50: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:39:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:39:51: #2 number of paired peaks: 25 WARNING @ Sat, 11 Dec 2021 11:39:51: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:39:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:39:54: 11000000 INFO @ Sat, 11 Dec 2021 11:40:00: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 11:40:05: 13000000 INFO @ Sat, 11 Dec 2021 11:40:11: 14000000 INFO @ Sat, 11 Dec 2021 11:40:16: 15000000 INFO @ Sat, 11 Dec 2021 11:40:19: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:40:19: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:40:19: #1 total tags in treatment: 15492725 INFO @ Sat, 11 Dec 2021 11:40:19: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:40:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:40:19: #1 tags after filtering in treatment: 15492725 INFO @ Sat, 11 Dec 2021 11:40:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:40:19: #1 finished! INFO @ Sat, 11 Dec 2021 11:40:19: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:40:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:40:20: #2 number of paired peaks: 25 WARNING @ Sat, 11 Dec 2021 11:40:20: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:40:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986162/SRX9986162.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。