Job ID = 14171336 SRX = SRX9986154 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 18254559 spots for SRR13591565/SRR13591565.sra Written 18254559 spots for SRR13591565/SRR13591565.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171809 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:00 18254559 reads; of these: 18254559 (100.00%) were unpaired; of these: 2132695 (11.68%) aligned 0 times 13185604 (72.23%) aligned exactly 1 time 2936260 (16.09%) aligned >1 times 88.32% overall alignment rate Time searching: 00:05:00 Overall time: 00:05:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3726905 / 16121864 = 0.2312 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:27:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:27:55: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:27:55: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:28:00: 1000000 INFO @ Sat, 11 Dec 2021 11:28:05: 2000000 INFO @ Sat, 11 Dec 2021 11:28:10: 3000000 INFO @ Sat, 11 Dec 2021 11:28:15: 4000000 INFO @ Sat, 11 Dec 2021 11:28:20: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:28:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:28:25: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:28:25: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:28:25: 6000000 INFO @ Sat, 11 Dec 2021 11:28:30: 1000000 INFO @ Sat, 11 Dec 2021 11:28:30: 7000000 INFO @ Sat, 11 Dec 2021 11:28:35: 2000000 INFO @ Sat, 11 Dec 2021 11:28:35: 8000000 INFO @ Sat, 11 Dec 2021 11:28:40: 3000000 INFO @ Sat, 11 Dec 2021 11:28:40: 9000000 INFO @ Sat, 11 Dec 2021 11:28:46: 4000000 INFO @ Sat, 11 Dec 2021 11:28:46: 10000000 INFO @ Sat, 11 Dec 2021 11:28:51: 5000000 INFO @ Sat, 11 Dec 2021 11:28:51: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:28:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:28:55: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:28:55: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:28:56: 6000000 INFO @ Sat, 11 Dec 2021 11:28:56: 12000000 INFO @ Sat, 11 Dec 2021 11:28:58: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:28:58: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:28:58: #1 total tags in treatment: 12394959 INFO @ Sat, 11 Dec 2021 11:28:58: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:28:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:28:59: #1 tags after filtering in treatment: 12394959 INFO @ Sat, 11 Dec 2021 11:28:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:28:59: #1 finished! INFO @ Sat, 11 Dec 2021 11:28:59: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:28:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:28:59: #2 number of paired peaks: 14 WARNING @ Sat, 11 Dec 2021 11:28:59: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:28:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:29:00: 1000000 INFO @ Sat, 11 Dec 2021 11:29:01: 7000000 INFO @ Sat, 11 Dec 2021 11:29:05: 2000000 INFO @ Sat, 11 Dec 2021 11:29:07: 8000000 INFO @ Sat, 11 Dec 2021 11:29:11: 3000000 INFO @ Sat, 11 Dec 2021 11:29:12: 9000000 INFO @ Sat, 11 Dec 2021 11:29:16: 4000000 INFO @ Sat, 11 Dec 2021 11:29:17: 10000000 INFO @ Sat, 11 Dec 2021 11:29:21: 5000000 INFO @ Sat, 11 Dec 2021 11:29:23: 11000000 INFO @ Sat, 11 Dec 2021 11:29:27: 6000000 INFO @ Sat, 11 Dec 2021 11:29:28: 12000000 INFO @ Sat, 11 Dec 2021 11:29:30: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:29:30: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:29:30: #1 total tags in treatment: 12394959 INFO @ Sat, 11 Dec 2021 11:29:30: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:29:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:29:30: #1 tags after filtering in treatment: 12394959 INFO @ Sat, 11 Dec 2021 11:29:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:29:30: #1 finished! INFO @ Sat, 11 Dec 2021 11:29:30: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:29:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:29:31: #2 number of paired peaks: 14 WARNING @ Sat, 11 Dec 2021 11:29:31: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:29:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:29:32: 7000000 INFO @ Sat, 11 Dec 2021 11:29:37: 8000000 INFO @ Sat, 11 Dec 2021 11:29:42: 9000000 INFO @ Sat, 11 Dec 2021 11:29:47: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 11:29:52: 11000000 INFO @ Sat, 11 Dec 2021 11:29:58: 12000000 INFO @ Sat, 11 Dec 2021 11:30:00: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:30:00: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:30:00: #1 total tags in treatment: 12394959 INFO @ Sat, 11 Dec 2021 11:30:00: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:30:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:30:00: #1 tags after filtering in treatment: 12394959 INFO @ Sat, 11 Dec 2021 11:30:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:30:00: #1 finished! INFO @ Sat, 11 Dec 2021 11:30:00: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:30:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:30:01: #2 number of paired peaks: 14 WARNING @ Sat, 11 Dec 2021 11:30:01: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 11:30:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986154/SRX9986154.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。