Job ID = 14171317 SRX = SRX9986147 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 11685877 spots for SRR13591510/SRR13591510.sra Written 11685877 spots for SRR13591510/SRR13591510.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171773 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:44 11685877 reads; of these: 11685877 (100.00%) were unpaired; of these: 1131637 (9.68%) aligned 0 times 8235828 (70.48%) aligned exactly 1 time 2318412 (19.84%) aligned >1 times 90.32% overall alignment rate Time searching: 00:03:44 Overall time: 00:03:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2232472 / 10554240 = 0.2115 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:20:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:20:42: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:20:42: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:20:51: 1000000 INFO @ Sat, 11 Dec 2021 11:20:59: 2000000 INFO @ Sat, 11 Dec 2021 11:21:07: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:21:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:21:12: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:21:12: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:21:16: 4000000 INFO @ Sat, 11 Dec 2021 11:21:20: 1000000 INFO @ Sat, 11 Dec 2021 11:21:24: 5000000 INFO @ Sat, 11 Dec 2021 11:21:28: 2000000 INFO @ Sat, 11 Dec 2021 11:21:32: 6000000 INFO @ Sat, 11 Dec 2021 11:21:37: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:21:41: 7000000 INFO @ Sat, 11 Dec 2021 11:21:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:21:42: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:21:42: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:21:45: 4000000 INFO @ Sat, 11 Dec 2021 11:21:49: 8000000 INFO @ Sat, 11 Dec 2021 11:21:51: 1000000 INFO @ Sat, 11 Dec 2021 11:21:52: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:21:52: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:21:52: #1 total tags in treatment: 8321768 INFO @ Sat, 11 Dec 2021 11:21:52: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:21:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:21:52: #1 tags after filtering in treatment: 8321768 INFO @ Sat, 11 Dec 2021 11:21:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:21:52: #1 finished! INFO @ Sat, 11 Dec 2021 11:21:52: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:21:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:21:53: #2 number of paired peaks: 1752 INFO @ Sat, 11 Dec 2021 11:21:53: start model_add_line... INFO @ Sat, 11 Dec 2021 11:21:53: start X-correlation... INFO @ Sat, 11 Dec 2021 11:21:53: end of X-cor INFO @ Sat, 11 Dec 2021 11:21:53: #2 finished! INFO @ Sat, 11 Dec 2021 11:21:53: #2 predicted fragment length is 147 bps INFO @ Sat, 11 Dec 2021 11:21:53: #2 alternative fragment length(s) may be 17,19,37,98,145,147,271,288,472,517 bps INFO @ Sat, 11 Dec 2021 11:21:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.05_model.r INFO @ Sat, 11 Dec 2021 11:21:53: #3 Call peaks... INFO @ Sat, 11 Dec 2021 11:21:53: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 11:21:54: 5000000 INFO @ Sat, 11 Dec 2021 11:21:59: 2000000 INFO @ Sat, 11 Dec 2021 11:22:02: 6000000 INFO @ Sat, 11 Dec 2021 11:22:07: 3000000 INFO @ Sat, 11 Dec 2021 11:22:10: 7000000 INFO @ Sat, 11 Dec 2021 11:22:16: 4000000 INFO @ Sat, 11 Dec 2021 11:22:18: 8000000 INFO @ Sat, 11 Dec 2021 11:22:18: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 11:22:20: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:22:20: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:22:20: #1 total tags in treatment: 8321768 INFO @ Sat, 11 Dec 2021 11:22:20: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:22:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:22:20: #1 tags after filtering in treatment: 8321768 INFO @ Sat, 11 Dec 2021 11:22:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:22:20: #1 finished! INFO @ Sat, 11 Dec 2021 11:22:20: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:22:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:22:21: #2 number of paired peaks: 1752 INFO @ Sat, 11 Dec 2021 11:22:21: start model_add_line... INFO @ Sat, 11 Dec 2021 11:22:21: start X-correlation... INFO @ Sat, 11 Dec 2021 11:22:21: end of X-cor INFO @ Sat, 11 Dec 2021 11:22:21: #2 finished! INFO @ Sat, 11 Dec 2021 11:22:21: #2 predicted fragment length is 147 bps INFO @ Sat, 11 Dec 2021 11:22:21: #2 alternative fragment length(s) may be 17,19,37,98,145,147,271,288,472,517 bps INFO @ Sat, 11 Dec 2021 11:22:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.10_model.r INFO @ Sat, 11 Dec 2021 11:22:21: #3 Call peaks... INFO @ Sat, 11 Dec 2021 11:22:21: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 11:22:24: 5000000 INFO @ Sat, 11 Dec 2021 11:22:32: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.05_peaks.xls INFO @ Sat, 11 Dec 2021 11:22:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 11:22:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.05_summits.bed INFO @ Sat, 11 Dec 2021 11:22:32: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (1065 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:22:32: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 11:22:40: 7000000 INFO @ Sat, 11 Dec 2021 11:22:47: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 11:22:48: 8000000 INFO @ Sat, 11 Dec 2021 11:22:50: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 11:22:50: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 11:22:50: #1 total tags in treatment: 8321768 INFO @ Sat, 11 Dec 2021 11:22:50: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:22:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:22:50: #1 tags after filtering in treatment: 8321768 INFO @ Sat, 11 Dec 2021 11:22:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 11:22:50: #1 finished! INFO @ Sat, 11 Dec 2021 11:22:50: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:22:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:22:51: #2 number of paired peaks: 1752 INFO @ Sat, 11 Dec 2021 11:22:51: start model_add_line... INFO @ Sat, 11 Dec 2021 11:22:51: start X-correlation... INFO @ Sat, 11 Dec 2021 11:22:51: end of X-cor INFO @ Sat, 11 Dec 2021 11:22:51: #2 finished! INFO @ Sat, 11 Dec 2021 11:22:51: #2 predicted fragment length is 147 bps INFO @ Sat, 11 Dec 2021 11:22:51: #2 alternative fragment length(s) may be 17,19,37,98,145,147,271,288,472,517 bps INFO @ Sat, 11 Dec 2021 11:22:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.20_model.r INFO @ Sat, 11 Dec 2021 11:22:51: #3 Call peaks... INFO @ Sat, 11 Dec 2021 11:22:51: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 11:22:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.10_peaks.xls INFO @ Sat, 11 Dec 2021 11:22:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 11:22:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.10_summits.bed INFO @ Sat, 11 Dec 2021 11:22:59: Done! pass1 - making usageList (11 chroms): 1 millis BigWig に変換しました。 pass2 - checking and writing primary data (331 records, 4 fields): 22 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 11:23:17: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 11:23:29: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.20_peaks.xls INFO @ Sat, 11 Dec 2021 11:23:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 11:23:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9986147/SRX9986147.20_summits.bed INFO @ Sat, 11 Dec 2021 11:23:29: Done! pass1 - making usageList (4 chroms): 1 millis pass2 - checking and writing primary data (66 records, 4 fields): 2 millis CompletedMACS2peakCalling