Job ID = 14171244 SRX = SRX9986123 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 22823812 spots for SRR13591542/SRR13591542.sra Written 22823812 spots for SRR13591542/SRR13591542.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171717 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:16 22823812 reads; of these: 22823812 (100.00%) were unpaired; of these: 2411425 (10.57%) aligned 0 times 16861550 (73.88%) aligned exactly 1 time 3550837 (15.56%) aligned >1 times 89.43% overall alignment rate Time searching: 00:07:17 Overall time: 00:07:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5145395 / 20412387 = 0.2521 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:56:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:56:35: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:56:35: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:56:44: 1000000 INFO @ Sat, 11 Dec 2021 10:56:52: 2000000 INFO @ Sat, 11 Dec 2021 10:56:59: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:57:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:57:05: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:57:05: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:57:07: 4000000 INFO @ Sat, 11 Dec 2021 10:57:15: 1000000 INFO @ Sat, 11 Dec 2021 10:57:16: 5000000 INFO @ Sat, 11 Dec 2021 10:57:23: 2000000 INFO @ Sat, 11 Dec 2021 10:57:24: 6000000 INFO @ Sat, 11 Dec 2021 10:57:32: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:57:33: 7000000 INFO @ Sat, 11 Dec 2021 10:57:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:57:35: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:57:35: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:57:40: 4000000 INFO @ Sat, 11 Dec 2021 10:57:42: 8000000 INFO @ Sat, 11 Dec 2021 10:57:45: 1000000 INFO @ Sat, 11 Dec 2021 10:57:50: 5000000 INFO @ Sat, 11 Dec 2021 10:57:51: 9000000 INFO @ Sat, 11 Dec 2021 10:57:55: 2000000 INFO @ Sat, 11 Dec 2021 10:57:58: 6000000 INFO @ Sat, 11 Dec 2021 10:58:01: 10000000 INFO @ Sat, 11 Dec 2021 10:58:04: 3000000 INFO @ Sat, 11 Dec 2021 10:58:08: 7000000 INFO @ Sat, 11 Dec 2021 10:58:10: 11000000 INFO @ Sat, 11 Dec 2021 10:58:14: 4000000 INFO @ Sat, 11 Dec 2021 10:58:18: 8000000 INFO @ Sat, 11 Dec 2021 10:58:21: 12000000 INFO @ Sat, 11 Dec 2021 10:58:23: 5000000 INFO @ Sat, 11 Dec 2021 10:58:28: 9000000 INFO @ Sat, 11 Dec 2021 10:58:30: 13000000 INFO @ Sat, 11 Dec 2021 10:58:33: 6000000 INFO @ Sat, 11 Dec 2021 10:58:38: 10000000 INFO @ Sat, 11 Dec 2021 10:58:39: 14000000 INFO @ Sat, 11 Dec 2021 10:58:43: 7000000 INFO @ Sat, 11 Dec 2021 10:58:47: 15000000 INFO @ Sat, 11 Dec 2021 10:58:47: 11000000 INFO @ Sat, 11 Dec 2021 10:58:50: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:58:50: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:58:50: #1 total tags in treatment: 15266992 INFO @ Sat, 11 Dec 2021 10:58:50: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:58:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:58:50: #1 tags after filtering in treatment: 15266992 INFO @ Sat, 11 Dec 2021 10:58:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:58:50: #1 finished! INFO @ Sat, 11 Dec 2021 10:58:50: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:58:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:58:51: #2 number of paired peaks: 43 WARNING @ Sat, 11 Dec 2021 10:58:51: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:58:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:58:52: 8000000 INFO @ Sat, 11 Dec 2021 10:58:56: 12000000 INFO @ Sat, 11 Dec 2021 10:59:01: 9000000 INFO @ Sat, 11 Dec 2021 10:59:05: 13000000 INFO @ Sat, 11 Dec 2021 10:59:10: 10000000 INFO @ Sat, 11 Dec 2021 10:59:14: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 10:59:18: 11000000 INFO @ Sat, 11 Dec 2021 10:59:22: 15000000 INFO @ Sat, 11 Dec 2021 10:59:24: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:59:24: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:59:24: #1 total tags in treatment: 15266992 INFO @ Sat, 11 Dec 2021 10:59:24: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:59:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:59:25: #1 tags after filtering in treatment: 15266992 INFO @ Sat, 11 Dec 2021 10:59:25: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:59:25: #1 finished! INFO @ Sat, 11 Dec 2021 10:59:25: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:59:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:59:26: 12000000 INFO @ Sat, 11 Dec 2021 10:59:26: #2 number of paired peaks: 43 WARNING @ Sat, 11 Dec 2021 10:59:26: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:59:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:59:34: 13000000 INFO @ Sat, 11 Dec 2021 10:59:42: 14000000 INFO @ Sat, 11 Dec 2021 10:59:50: 15000000 INFO @ Sat, 11 Dec 2021 10:59:52: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:59:52: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:59:52: #1 total tags in treatment: 15266992 INFO @ Sat, 11 Dec 2021 10:59:52: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:59:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:59:52: #1 tags after filtering in treatment: 15266992 INFO @ Sat, 11 Dec 2021 10:59:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:59:52: #1 finished! INFO @ Sat, 11 Dec 2021 10:59:52: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:59:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:59:54: #2 number of paired peaks: 43 WARNING @ Sat, 11 Dec 2021 10:59:54: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:59:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986123/SRX9986123.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。