Job ID = 14171242 SRX = SRX9986121 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 11921957 spots for SRR13591540/SRR13591540.sra Written 11921957 spots for SRR13591540/SRR13591540.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171704 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:40 11921957 reads; of these: 11921957 (100.00%) were unpaired; of these: 900918 (7.56%) aligned 0 times 8066498 (67.66%) aligned exactly 1 time 2954541 (24.78%) aligned >1 times 92.44% overall alignment rate Time searching: 00:03:40 Overall time: 00:03:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1589626 / 11021039 = 0.1442 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:48:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:48:34: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:48:34: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:48:39: 1000000 INFO @ Sat, 11 Dec 2021 10:48:44: 2000000 INFO @ Sat, 11 Dec 2021 10:48:49: 3000000 INFO @ Sat, 11 Dec 2021 10:48:54: 4000000 INFO @ Sat, 11 Dec 2021 10:49:00: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:49:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:49:04: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:49:04: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:49:04: 6000000 INFO @ Sat, 11 Dec 2021 10:49:09: 1000000 INFO @ Sat, 11 Dec 2021 10:49:10: 7000000 INFO @ Sat, 11 Dec 2021 10:49:15: 2000000 INFO @ Sat, 11 Dec 2021 10:49:15: 8000000 INFO @ Sat, 11 Dec 2021 10:49:19: 3000000 INFO @ Sat, 11 Dec 2021 10:49:20: 9000000 INFO @ Sat, 11 Dec 2021 10:49:22: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:49:22: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:49:22: #1 total tags in treatment: 9431413 INFO @ Sat, 11 Dec 2021 10:49:22: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:49:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:49:22: #1 tags after filtering in treatment: 9431413 INFO @ Sat, 11 Dec 2021 10:49:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:49:22: #1 finished! INFO @ Sat, 11 Dec 2021 10:49:22: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:49:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:49:23: #2 number of paired peaks: 66 WARNING @ Sat, 11 Dec 2021 10:49:23: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:49:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:49:24: 4000000 INFO @ Sat, 11 Dec 2021 10:49:29: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:49:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:49:34: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:49:34: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:49:34: 6000000 INFO @ Sat, 11 Dec 2021 10:49:39: 7000000 INFO @ Sat, 11 Dec 2021 10:49:39: 1000000 INFO @ Sat, 11 Dec 2021 10:49:44: 8000000 INFO @ Sat, 11 Dec 2021 10:49:45: 2000000 INFO @ Sat, 11 Dec 2021 10:49:48: 9000000 INFO @ Sat, 11 Dec 2021 10:49:50: 3000000 INFO @ Sat, 11 Dec 2021 10:49:50: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:49:50: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:49:50: #1 total tags in treatment: 9431413 INFO @ Sat, 11 Dec 2021 10:49:50: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:49:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:49:51: #1 tags after filtering in treatment: 9431413 INFO @ Sat, 11 Dec 2021 10:49:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:49:51: #1 finished! INFO @ Sat, 11 Dec 2021 10:49:51: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:49:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:49:51: #2 number of paired peaks: 66 WARNING @ Sat, 11 Dec 2021 10:49:51: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:49:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:49:55: 4000000 INFO @ Sat, 11 Dec 2021 10:50:00: 5000000 INFO @ Sat, 11 Dec 2021 10:50:05: 6000000 INFO @ Sat, 11 Dec 2021 10:50:10: 7000000 INFO @ Sat, 11 Dec 2021 10:50:15: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 10:50:20: 9000000 INFO @ Sat, 11 Dec 2021 10:50:22: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:50:22: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:50:22: #1 total tags in treatment: 9431413 INFO @ Sat, 11 Dec 2021 10:50:22: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:50:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:50:22: #1 tags after filtering in treatment: 9431413 INFO @ Sat, 11 Dec 2021 10:50:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:50:22: #1 finished! INFO @ Sat, 11 Dec 2021 10:50:22: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:50:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:50:23: #2 number of paired peaks: 66 WARNING @ Sat, 11 Dec 2021 10:50:23: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:50:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986121/SRX9986121.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。