Job ID = 14171196 SRX = SRX9986117 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 18689220 spots for SRR13591536/SRR13591536.sra Written 18689220 spots for SRR13591536/SRR13591536.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171678 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:50 18689220 reads; of these: 18689220 (100.00%) were unpaired; of these: 1417017 (7.58%) aligned 0 times 12435347 (66.54%) aligned exactly 1 time 4836856 (25.88%) aligned >1 times 92.42% overall alignment rate Time searching: 00:05:50 Overall time: 00:05:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3026426 / 17272203 = 0.1752 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:41:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:41:32: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:41:32: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:41:39: 1000000 INFO @ Sat, 11 Dec 2021 10:41:44: 2000000 INFO @ Sat, 11 Dec 2021 10:41:50: 3000000 INFO @ Sat, 11 Dec 2021 10:41:56: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:42:02: 5000000 INFO @ Sat, 11 Dec 2021 10:42:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:42:02: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:42:02: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:42:09: 6000000 INFO @ Sat, 11 Dec 2021 10:42:10: 1000000 INFO @ Sat, 11 Dec 2021 10:42:16: 7000000 INFO @ Sat, 11 Dec 2021 10:42:16: 2000000 INFO @ Sat, 11 Dec 2021 10:42:22: 8000000 INFO @ Sat, 11 Dec 2021 10:42:23: 3000000 INFO @ Sat, 11 Dec 2021 10:42:29: 9000000 INFO @ Sat, 11 Dec 2021 10:42:29: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:42:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:42:32: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:42:32: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:42:35: 10000000 INFO @ Sat, 11 Dec 2021 10:42:36: 5000000 INFO @ Sat, 11 Dec 2021 10:42:39: 1000000 INFO @ Sat, 11 Dec 2021 10:42:42: 11000000 INFO @ Sat, 11 Dec 2021 10:42:42: 6000000 INFO @ Sat, 11 Dec 2021 10:42:46: 2000000 INFO @ Sat, 11 Dec 2021 10:42:48: 12000000 INFO @ Sat, 11 Dec 2021 10:42:49: 7000000 INFO @ Sat, 11 Dec 2021 10:42:53: 3000000 INFO @ Sat, 11 Dec 2021 10:42:55: 13000000 INFO @ Sat, 11 Dec 2021 10:42:56: 8000000 INFO @ Sat, 11 Dec 2021 10:43:00: 4000000 INFO @ Sat, 11 Dec 2021 10:43:02: 14000000 INFO @ Sat, 11 Dec 2021 10:43:03: 9000000 INFO @ Sat, 11 Dec 2021 10:43:04: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:43:04: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:43:04: #1 total tags in treatment: 14245777 INFO @ Sat, 11 Dec 2021 10:43:04: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:43:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:43:04: #1 tags after filtering in treatment: 14245777 INFO @ Sat, 11 Dec 2021 10:43:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:43:04: #1 finished! INFO @ Sat, 11 Dec 2021 10:43:04: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:43:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:43:05: #2 number of paired peaks: 43 WARNING @ Sat, 11 Dec 2021 10:43:05: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:43:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:43:07: 5000000 INFO @ Sat, 11 Dec 2021 10:43:09: 10000000 INFO @ Sat, 11 Dec 2021 10:43:13: 6000000 INFO @ Sat, 11 Dec 2021 10:43:16: 11000000 INFO @ Sat, 11 Dec 2021 10:43:20: 7000000 INFO @ Sat, 11 Dec 2021 10:43:23: 12000000 INFO @ Sat, 11 Dec 2021 10:43:27: 8000000 INFO @ Sat, 11 Dec 2021 10:43:29: 13000000 INFO @ Sat, 11 Dec 2021 10:43:34: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 10:43:36: 14000000 INFO @ Sat, 11 Dec 2021 10:43:38: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:43:38: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:43:38: #1 total tags in treatment: 14245777 INFO @ Sat, 11 Dec 2021 10:43:38: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:43:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:43:38: #1 tags after filtering in treatment: 14245777 INFO @ Sat, 11 Dec 2021 10:43:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:43:38: #1 finished! INFO @ Sat, 11 Dec 2021 10:43:38: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:43:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:43:39: #2 number of paired peaks: 43 WARNING @ Sat, 11 Dec 2021 10:43:39: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:43:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:43:40: 10000000 INFO @ Sat, 11 Dec 2021 10:43:47: 11000000 INFO @ Sat, 11 Dec 2021 10:43:53: 12000000 INFO @ Sat, 11 Dec 2021 10:43:59: 13000000 INFO @ Sat, 11 Dec 2021 10:44:06: 14000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 10:44:07: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:44:07: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:44:07: #1 total tags in treatment: 14245777 INFO @ Sat, 11 Dec 2021 10:44:07: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:44:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:44:07: #1 tags after filtering in treatment: 14245777 INFO @ Sat, 11 Dec 2021 10:44:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:44:07: #1 finished! INFO @ Sat, 11 Dec 2021 10:44:07: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:44:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:44:08: #2 number of paired peaks: 43 WARNING @ Sat, 11 Dec 2021 10:44:08: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:44:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986117/SRX9986117.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling