Job ID = 14171188 SRX = SRX9986114 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 15032490 spots for SRR13591533/SRR13591533.sra Written 15032490 spots for SRR13591533/SRR13591533.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171671 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:50 15032490 reads; of these: 15032490 (100.00%) were unpaired; of these: 1823169 (12.13%) aligned 0 times 10489987 (69.78%) aligned exactly 1 time 2719334 (18.09%) aligned >1 times 87.87% overall alignment rate Time searching: 00:05:51 Overall time: 00:05:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2877114 / 13209321 = 0.2178 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:38:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:38:52: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:38:52: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:39:00: 1000000 INFO @ Sat, 11 Dec 2021 10:39:08: 2000000 INFO @ Sat, 11 Dec 2021 10:39:16: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:39:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:39:21: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:39:21: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:39:24: 4000000 INFO @ Sat, 11 Dec 2021 10:39:30: 1000000 INFO @ Sat, 11 Dec 2021 10:39:32: 5000000 INFO @ Sat, 11 Dec 2021 10:39:40: 2000000 INFO @ Sat, 11 Dec 2021 10:39:41: 6000000 INFO @ Sat, 11 Dec 2021 10:39:48: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:39:50: 7000000 INFO @ Sat, 11 Dec 2021 10:39:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:39:51: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:39:51: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:39:57: 4000000 INFO @ Sat, 11 Dec 2021 10:39:59: 8000000 INFO @ Sat, 11 Dec 2021 10:40:01: 1000000 INFO @ Sat, 11 Dec 2021 10:40:07: 5000000 INFO @ Sat, 11 Dec 2021 10:40:09: 9000000 INFO @ Sat, 11 Dec 2021 10:40:11: 2000000 INFO @ Sat, 11 Dec 2021 10:40:17: 6000000 INFO @ Sat, 11 Dec 2021 10:40:18: 10000000 INFO @ Sat, 11 Dec 2021 10:40:21: 3000000 INFO @ Sat, 11 Dec 2021 10:40:21: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:40:21: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:40:21: #1 total tags in treatment: 10332207 INFO @ Sat, 11 Dec 2021 10:40:21: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:40:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:40:22: #1 tags after filtering in treatment: 10332207 INFO @ Sat, 11 Dec 2021 10:40:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:40:22: #1 finished! INFO @ Sat, 11 Dec 2021 10:40:22: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:40:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:40:23: #2 number of paired peaks: 49 WARNING @ Sat, 11 Dec 2021 10:40:23: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:40:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:40:27: 7000000 INFO @ Sat, 11 Dec 2021 10:40:31: 4000000 INFO @ Sat, 11 Dec 2021 10:40:37: 8000000 INFO @ Sat, 11 Dec 2021 10:40:41: 5000000 INFO @ Sat, 11 Dec 2021 10:40:47: 9000000 INFO @ Sat, 11 Dec 2021 10:40:51: 6000000 INFO @ Sat, 11 Dec 2021 10:40:57: 10000000 INFO @ Sat, 11 Dec 2021 10:41:00: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:41:00: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:41:00: #1 total tags in treatment: 10332207 INFO @ Sat, 11 Dec 2021 10:41:00: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:41:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 10:41:01: #1 tags after filtering in treatment: 10332207 INFO @ Sat, 11 Dec 2021 10:41:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:41:01: #1 finished! INFO @ Sat, 11 Dec 2021 10:41:01: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:41:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:41:01: 7000000 INFO @ Sat, 11 Dec 2021 10:41:02: #2 number of paired peaks: 49 WARNING @ Sat, 11 Dec 2021 10:41:02: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:41:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:41:10: 8000000 INFO @ Sat, 11 Dec 2021 10:41:19: 9000000 INFO @ Sat, 11 Dec 2021 10:41:29: 10000000 INFO @ Sat, 11 Dec 2021 10:41:32: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:41:32: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:41:32: #1 total tags in treatment: 10332207 INFO @ Sat, 11 Dec 2021 10:41:32: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:41:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:41:32: #1 tags after filtering in treatment: 10332207 INFO @ Sat, 11 Dec 2021 10:41:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:41:32: #1 finished! INFO @ Sat, 11 Dec 2021 10:41:32: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:41:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:41:33: #2 number of paired peaks: 49 WARNING @ Sat, 11 Dec 2021 10:41:33: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:41:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986114/SRX9986114.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。