Job ID = 14171186 SRX = SRX9986112 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 13350561 spots for SRR13591531/SRR13591531.sra Written 13350561 spots for SRR13591531/SRR13591531.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171655 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:03:53 13350561 reads; of these: 13350561 (100.00%) were unpaired; of these: 1876639 (14.06%) aligned 0 times 9474146 (70.96%) aligned exactly 1 time 1999776 (14.98%) aligned >1 times 85.94% overall alignment rate Time searching: 00:03:54 Overall time: 00:03:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2047387 / 11473922 = 0.1784 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:34:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:34:37: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:34:37: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:34:43: 1000000 INFO @ Sat, 11 Dec 2021 10:34:48: 2000000 INFO @ Sat, 11 Dec 2021 10:34:54: 3000000 INFO @ Sat, 11 Dec 2021 10:34:59: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:35:05: 5000000 INFO @ Sat, 11 Dec 2021 10:35:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:35:07: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:35:07: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:35:10: 6000000 INFO @ Sat, 11 Dec 2021 10:35:15: 1000000 INFO @ Sat, 11 Dec 2021 10:35:16: 7000000 INFO @ Sat, 11 Dec 2021 10:35:22: 8000000 INFO @ Sat, 11 Dec 2021 10:35:22: 2000000 INFO @ Sat, 11 Dec 2021 10:35:28: 9000000 INFO @ Sat, 11 Dec 2021 10:35:30: 3000000 INFO @ Sat, 11 Dec 2021 10:35:31: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:35:31: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:35:31: #1 total tags in treatment: 9426535 INFO @ Sat, 11 Dec 2021 10:35:31: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:35:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:35:31: #1 tags after filtering in treatment: 9426535 INFO @ Sat, 11 Dec 2021 10:35:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:35:31: #1 finished! INFO @ Sat, 11 Dec 2021 10:35:31: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:35:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:35:32: #2 number of paired peaks: 46 WARNING @ Sat, 11 Dec 2021 10:35:32: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:35:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:35:37: 4000000 INFO @ Sat, 11 Dec 2021 10:35:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:35:37: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:35:37: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:35:43: 1000000 INFO @ Sat, 11 Dec 2021 10:35:45: 5000000 INFO @ Sat, 11 Dec 2021 10:35:48: 2000000 INFO @ Sat, 11 Dec 2021 10:35:52: 6000000 INFO @ Sat, 11 Dec 2021 10:35:54: 3000000 INFO @ Sat, 11 Dec 2021 10:36:00: 4000000 INFO @ Sat, 11 Dec 2021 10:36:00: 7000000 INFO @ Sat, 11 Dec 2021 10:36:06: 5000000 INFO @ Sat, 11 Dec 2021 10:36:08: 8000000 INFO @ Sat, 11 Dec 2021 10:36:11: 6000000 INFO @ Sat, 11 Dec 2021 10:36:15: 9000000 INFO @ Sat, 11 Dec 2021 10:36:17: 7000000 INFO @ Sat, 11 Dec 2021 10:36:18: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:36:18: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:36:18: #1 total tags in treatment: 9426535 INFO @ Sat, 11 Dec 2021 10:36:18: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:36:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:36:18: #1 tags after filtering in treatment: 9426535 INFO @ Sat, 11 Dec 2021 10:36:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:36:18: #1 finished! INFO @ Sat, 11 Dec 2021 10:36:18: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:36:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:36:19: #2 number of paired peaks: 46 WARNING @ Sat, 11 Dec 2021 10:36:19: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:36:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:36:22: 8000000 INFO @ Sat, 11 Dec 2021 10:36:27: 9000000 INFO @ Sat, 11 Dec 2021 10:36:29: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:36:29: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:36:29: #1 total tags in treatment: 9426535 INFO @ Sat, 11 Dec 2021 10:36:29: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:36:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:36:30: #1 tags after filtering in treatment: 9426535 INFO @ Sat, 11 Dec 2021 10:36:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:36:30: #1 finished! INFO @ Sat, 11 Dec 2021 10:36:30: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:36:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:36:30: #2 number of paired peaks: 46 WARNING @ Sat, 11 Dec 2021 10:36:30: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:36:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986112/SRX9986112.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。