Job ID = 14171185 SRX = SRX9986111 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 27353650 spots for SRR13591530/SRR13591530.sra Written 27353650 spots for SRR13591530/SRR13591530.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171672 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:07:42 27353650 reads; of these: 27353650 (100.00%) were unpaired; of these: 2352996 (8.60%) aligned 0 times 18096161 (66.16%) aligned exactly 1 time 6904493 (25.24%) aligned >1 times 91.40% overall alignment rate Time searching: 00:07:43 Overall time: 00:07:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5656232 / 25000654 = 0.2262 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:40:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:40:08: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:40:08: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:40:13: 1000000 INFO @ Sat, 11 Dec 2021 10:40:18: 2000000 INFO @ Sat, 11 Dec 2021 10:40:23: 3000000 INFO @ Sat, 11 Dec 2021 10:40:29: 4000000 INFO @ Sat, 11 Dec 2021 10:40:34: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:40:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:40:38: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:40:38: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:40:39: 6000000 INFO @ Sat, 11 Dec 2021 10:40:45: 7000000 INFO @ Sat, 11 Dec 2021 10:40:45: 1000000 INFO @ Sat, 11 Dec 2021 10:40:51: 8000000 INFO @ Sat, 11 Dec 2021 10:40:52: 2000000 INFO @ Sat, 11 Dec 2021 10:40:57: 9000000 INFO @ Sat, 11 Dec 2021 10:40:59: 3000000 INFO @ Sat, 11 Dec 2021 10:41:03: 10000000 INFO @ Sat, 11 Dec 2021 10:41:06: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:41:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:41:08: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:41:08: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:41:09: 11000000 INFO @ Sat, 11 Dec 2021 10:41:13: 5000000 INFO @ Sat, 11 Dec 2021 10:41:14: 1000000 INFO @ Sat, 11 Dec 2021 10:41:15: 12000000 INFO @ Sat, 11 Dec 2021 10:41:20: 6000000 INFO @ Sat, 11 Dec 2021 10:41:20: 2000000 INFO @ Sat, 11 Dec 2021 10:41:21: 13000000 INFO @ Sat, 11 Dec 2021 10:41:26: 3000000 INFO @ Sat, 11 Dec 2021 10:41:27: 7000000 INFO @ Sat, 11 Dec 2021 10:41:27: 14000000 INFO @ Sat, 11 Dec 2021 10:41:32: 4000000 INFO @ Sat, 11 Dec 2021 10:41:33: 15000000 INFO @ Sat, 11 Dec 2021 10:41:33: 8000000 INFO @ Sat, 11 Dec 2021 10:41:39: 5000000 INFO @ Sat, 11 Dec 2021 10:41:39: 16000000 INFO @ Sat, 11 Dec 2021 10:41:40: 9000000 INFO @ Sat, 11 Dec 2021 10:41:45: 6000000 INFO @ Sat, 11 Dec 2021 10:41:45: 17000000 INFO @ Sat, 11 Dec 2021 10:41:47: 10000000 INFO @ Sat, 11 Dec 2021 10:41:51: 18000000 INFO @ Sat, 11 Dec 2021 10:41:51: 7000000 INFO @ Sat, 11 Dec 2021 10:41:54: 11000000 INFO @ Sat, 11 Dec 2021 10:41:57: 19000000 INFO @ Sat, 11 Dec 2021 10:41:58: 8000000 INFO @ Sat, 11 Dec 2021 10:41:59: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:41:59: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:41:59: #1 total tags in treatment: 19344422 INFO @ Sat, 11 Dec 2021 10:41:59: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:41:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:42:00: #1 tags after filtering in treatment: 19344422 INFO @ Sat, 11 Dec 2021 10:42:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:42:00: #1 finished! INFO @ Sat, 11 Dec 2021 10:42:00: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:42:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:42:01: 12000000 INFO @ Sat, 11 Dec 2021 10:42:01: #2 number of paired peaks: 17 WARNING @ Sat, 11 Dec 2021 10:42:01: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:42:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:42:04: 9000000 INFO @ Sat, 11 Dec 2021 10:42:08: 13000000 INFO @ Sat, 11 Dec 2021 10:42:10: 10000000 INFO @ Sat, 11 Dec 2021 10:42:14: 14000000 INFO @ Sat, 11 Dec 2021 10:42:16: 11000000 INFO @ Sat, 11 Dec 2021 10:42:21: 15000000 INFO @ Sat, 11 Dec 2021 10:42:22: 12000000 INFO @ Sat, 11 Dec 2021 10:42:28: 13000000 INFO @ Sat, 11 Dec 2021 10:42:28: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 10:42:34: 14000000 INFO @ Sat, 11 Dec 2021 10:42:35: 17000000 INFO @ Sat, 11 Dec 2021 10:42:40: 15000000 INFO @ Sat, 11 Dec 2021 10:42:42: 18000000 INFO @ Sat, 11 Dec 2021 10:42:46: 16000000 INFO @ Sat, 11 Dec 2021 10:42:49: 19000000 INFO @ Sat, 11 Dec 2021 10:42:51: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:42:51: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:42:51: #1 total tags in treatment: 19344422 INFO @ Sat, 11 Dec 2021 10:42:51: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:42:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:42:52: #1 tags after filtering in treatment: 19344422 INFO @ Sat, 11 Dec 2021 10:42:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:42:52: #1 finished! INFO @ Sat, 11 Dec 2021 10:42:52: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:42:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:42:52: 17000000 INFO @ Sat, 11 Dec 2021 10:42:53: #2 number of paired peaks: 17 WARNING @ Sat, 11 Dec 2021 10:42:53: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:42:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:42:58: 18000000 INFO @ Sat, 11 Dec 2021 10:43:04: 19000000 INFO @ Sat, 11 Dec 2021 10:43:06: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 10:43:06: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 10:43:06: #1 total tags in treatment: 19344422 INFO @ Sat, 11 Dec 2021 10:43:06: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:43:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:43:06: #1 tags after filtering in treatment: 19344422 INFO @ Sat, 11 Dec 2021 10:43:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 10:43:06: #1 finished! INFO @ Sat, 11 Dec 2021 10:43:06: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:43:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:43:07: #2 number of paired peaks: 17 WARNING @ Sat, 11 Dec 2021 10:43:07: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 10:43:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9986111/SRX9986111.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。