Job ID = 10924637 sra ファイルのダウンロード中... Completed: 239416K bytes transferred in 6 seconds (303904K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 8573437 spots for /home/okishinya/chipatlas/results/dm3/SRX994749/SRR1973467.sra Written 8573437 spots for /home/okishinya/chipatlas/results/dm3/SRX994749/SRR1973467.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:31 8573437 reads; of these: 8573437 (100.00%) were unpaired; of these: 357624 (4.17%) aligned 0 times 6021839 (70.24%) aligned exactly 1 time 2193974 (25.59%) aligned >1 times 95.83% overall alignment rate Time searching: 00:04:32 Overall time: 00:04:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1953372 / 8215813 = 0.2378 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 06 Aug 2018 10:33:20: # Command line: callpeak -t SRX994749.bam -f BAM -g dm -n SRX994749.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX994749.10 # format = BAM # ChIP-seq file = ['SRX994749.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Aug 2018 10:33:20: # Command line: callpeak -t SRX994749.bam -f BAM -g dm -n SRX994749.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX994749.20 # format = BAM # ChIP-seq file = ['SRX994749.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Aug 2018 10:33:20: # Command line: callpeak -t SRX994749.bam -f BAM -g dm -n SRX994749.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX994749.05 # format = BAM # ChIP-seq file = ['SRX994749.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Aug 2018 10:33:20: #1 read tag files... INFO @ Mon, 06 Aug 2018 10:33:20: #1 read tag files... INFO @ Mon, 06 Aug 2018 10:33:20: #1 read tag files... INFO @ Mon, 06 Aug 2018 10:33:20: #1 read treatment tags... INFO @ Mon, 06 Aug 2018 10:33:20: #1 read treatment tags... INFO @ Mon, 06 Aug 2018 10:33:20: #1 read treatment tags... INFO @ Mon, 06 Aug 2018 10:33:28: 1000000 INFO @ Mon, 06 Aug 2018 10:33:28: 1000000 INFO @ Mon, 06 Aug 2018 10:33:28: 1000000 INFO @ Mon, 06 Aug 2018 10:33:35: 2000000 INFO @ Mon, 06 Aug 2018 10:33:36: 2000000 INFO @ Mon, 06 Aug 2018 10:33:36: 2000000 INFO @ Mon, 06 Aug 2018 10:33:43: 3000000 INFO @ Mon, 06 Aug 2018 10:33:43: 3000000 INFO @ Mon, 06 Aug 2018 10:33:43: 3000000 INFO @ Mon, 06 Aug 2018 10:33:50: 4000000 INFO @ Mon, 06 Aug 2018 10:33:51: 4000000 INFO @ Mon, 06 Aug 2018 10:33:51: 4000000 INFO @ Mon, 06 Aug 2018 10:33:57: 5000000 INFO @ Mon, 06 Aug 2018 10:33:58: 5000000 INFO @ Mon, 06 Aug 2018 10:33:58: 5000000 INFO @ Mon, 06 Aug 2018 10:34:05: 6000000 INFO @ Mon, 06 Aug 2018 10:34:06: 6000000 INFO @ Mon, 06 Aug 2018 10:34:06: 6000000 INFO @ Mon, 06 Aug 2018 10:34:07: #1 tag size is determined as 75 bps INFO @ Mon, 06 Aug 2018 10:34:07: #1 tag size = 75 INFO @ Mon, 06 Aug 2018 10:34:07: #1 total tags in treatment: 6262441 INFO @ Mon, 06 Aug 2018 10:34:07: #1 user defined the maximum tags... INFO @ Mon, 06 Aug 2018 10:34:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Aug 2018 10:34:07: #1 tags after filtering in treatment: 6262441 INFO @ Mon, 06 Aug 2018 10:34:07: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Aug 2018 10:34:07: #1 finished! INFO @ Mon, 06 Aug 2018 10:34:07: #2 Build Peak Model... INFO @ Mon, 06 Aug 2018 10:34:07: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Aug 2018 10:34:07: #2 number of paired peaks: 1335 INFO @ Mon, 06 Aug 2018 10:34:07: start model_add_line... INFO @ Mon, 06 Aug 2018 10:34:07: start X-correlation... INFO @ Mon, 06 Aug 2018 10:34:08: end of X-cor INFO @ Mon, 06 Aug 2018 10:34:08: #2 finished! INFO @ Mon, 06 Aug 2018 10:34:08: #2 predicted fragment length is 118 bps INFO @ Mon, 06 Aug 2018 10:34:08: #2 alternative fragment length(s) may be 118 bps INFO @ Mon, 06 Aug 2018 10:34:08: #2.2 Generate R script for model : SRX994749.05_model.r WARNING @ Mon, 06 Aug 2018 10:34:08: #2 Since the d (118) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 06 Aug 2018 10:34:08: #2 You may need to consider one of the other alternative d(s): 118 WARNING @ Mon, 06 Aug 2018 10:34:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 06 Aug 2018 10:34:08: #3 Call peaks... INFO @ Mon, 06 Aug 2018 10:34:08: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 06 Aug 2018 10:34:08: #1 tag size is determined as 75 bps INFO @ Mon, 06 Aug 2018 10:34:08: #1 tag size = 75 INFO @ Mon, 06 Aug 2018 10:34:08: #1 total tags in treatment: 6262441 INFO @ Mon, 06 Aug 2018 10:34:08: #1 user defined the maximum tags... INFO @ Mon, 06 Aug 2018 10:34:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Aug 2018 10:34:08: #1 tags after filtering in treatment: 6262441 INFO @ Mon, 06 Aug 2018 10:34:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Aug 2018 10:34:08: #1 finished! INFO @ Mon, 06 Aug 2018 10:34:08: #2 Build Peak Model... INFO @ Mon, 06 Aug 2018 10:34:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Aug 2018 10:34:08: #1 tag size is determined as 75 bps INFO @ Mon, 06 Aug 2018 10:34:08: #1 tag size = 75 INFO @ Mon, 06 Aug 2018 10:34:08: #1 total tags in treatment: 6262441 INFO @ Mon, 06 Aug 2018 10:34:08: #1 user defined the maximum tags... INFO @ Mon, 06 Aug 2018 10:34:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Aug 2018 10:34:08: #1 tags after filtering in treatment: 6262441 INFO @ Mon, 06 Aug 2018 10:34:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Aug 2018 10:34:08: #1 finished! INFO @ Mon, 06 Aug 2018 10:34:08: #2 Build Peak Model... INFO @ Mon, 06 Aug 2018 10:34:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Aug 2018 10:34:08: #2 number of paired peaks: 1335 INFO @ Mon, 06 Aug 2018 10:34:08: start model_add_line... INFO @ Mon, 06 Aug 2018 10:34:09: start X-correlation... INFO @ Mon, 06 Aug 2018 10:34:09: end of X-cor INFO @ Mon, 06 Aug 2018 10:34:09: #2 finished! INFO @ Mon, 06 Aug 2018 10:34:09: #2 predicted fragment length is 118 bps INFO @ Mon, 06 Aug 2018 10:34:09: #2 alternative fragment length(s) may be 118 bps INFO @ Mon, 06 Aug 2018 10:34:09: #2.2 Generate R script for model : SRX994749.10_model.r WARNING @ Mon, 06 Aug 2018 10:34:09: #2 Since the d (118) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 06 Aug 2018 10:34:09: #2 You may need to consider one of the other alternative d(s): 118 WARNING @ Mon, 06 Aug 2018 10:34:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 06 Aug 2018 10:34:09: #3 Call peaks... INFO @ Mon, 06 Aug 2018 10:34:09: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 06 Aug 2018 10:34:09: #2 number of paired peaks: 1335 INFO @ Mon, 06 Aug 2018 10:34:09: start model_add_line... INFO @ Mon, 06 Aug 2018 10:34:09: start X-correlation... INFO @ Mon, 06 Aug 2018 10:34:09: end of X-cor INFO @ Mon, 06 Aug 2018 10:34:09: #2 finished! INFO @ Mon, 06 Aug 2018 10:34:09: #2 predicted fragment length is 118 bps INFO @ Mon, 06 Aug 2018 10:34:09: #2 alternative fragment length(s) may be 118 bps INFO @ Mon, 06 Aug 2018 10:34:09: #2.2 Generate R script for model : SRX994749.20_model.r WARNING @ Mon, 06 Aug 2018 10:34:09: #2 Since the d (118) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 06 Aug 2018 10:34:09: #2 You may need to consider one of the other alternative d(s): 118 WARNING @ Mon, 06 Aug 2018 10:34:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 06 Aug 2018 10:34:09: #3 Call peaks... INFO @ Mon, 06 Aug 2018 10:34:09: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 06 Aug 2018 10:34:23: #3 Call peaks for each chromosome... INFO @ Mon, 06 Aug 2018 10:34:24: #3 Call peaks for each chromosome... INFO @ Mon, 06 Aug 2018 10:34:25: #3 Call peaks for each chromosome... INFO @ Mon, 06 Aug 2018 10:34:31: #4 Write output xls file... SRX994749.05_peaks.xls INFO @ Mon, 06 Aug 2018 10:34:31: #4 Write peak in narrowPeak format file... SRX994749.05_peaks.narrowPeak INFO @ Mon, 06 Aug 2018 10:34:31: #4 Write summits bed file... SRX994749.05_summits.bed INFO @ Mon, 06 Aug 2018 10:34:31: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (2573 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Mon, 06 Aug 2018 10:34:31: #4 Write output xls file... SRX994749.10_peaks.xls INFO @ Mon, 06 Aug 2018 10:34:31: #4 Write peak in narrowPeak format file... SRX994749.10_peaks.narrowPeak INFO @ Mon, 06 Aug 2018 10:34:31: #4 Write summits bed file... SRX994749.10_summits.bed INFO @ Mon, 06 Aug 2018 10:34:31: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (1446 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Mon, 06 Aug 2018 10:34:33: #4 Write output xls file... SRX994749.20_peaks.xls INFO @ Mon, 06 Aug 2018 10:34:33: #4 Write peak in narrowPeak format file... SRX994749.20_peaks.narrowPeak INFO @ Mon, 06 Aug 2018 10:34:33: #4 Write summits bed file... SRX994749.20_summits.bed INFO @ Mon, 06 Aug 2018 10:34:33: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (929 records, 4 fields): 750 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。