Job ID = 6528527 SRX = SRX992690 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:18:47 prefetch.2.10.7: 1) Downloading 'SRR1970917'... 2020-06-29T15:18:47 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:21:10 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:21:10 prefetch.2.10.7: 1) 'SRR1970917' was downloaded successfully Read 32113401 spots for SRR1970917/SRR1970917.sra Written 32113401 spots for SRR1970917/SRR1970917.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:41 32113401 reads; of these: 32113401 (100.00%) were unpaired; of these: 10811950 (33.67%) aligned 0 times 15482249 (48.21%) aligned exactly 1 time 5819202 (18.12%) aligned >1 times 66.33% overall alignment rate Time searching: 00:08:41 Overall time: 00:08:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2621991 / 21301451 = 0.1231 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:44:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:44:05: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:44:05: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:44:10: 1000000 INFO @ Tue, 30 Jun 2020 00:44:15: 2000000 INFO @ Tue, 30 Jun 2020 00:44:20: 3000000 INFO @ Tue, 30 Jun 2020 00:44:25: 4000000 INFO @ Tue, 30 Jun 2020 00:44:30: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:44:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:44:35: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:44:35: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:44:35: 6000000 INFO @ Tue, 30 Jun 2020 00:44:40: 1000000 INFO @ Tue, 30 Jun 2020 00:44:41: 7000000 INFO @ Tue, 30 Jun 2020 00:44:46: 2000000 INFO @ Tue, 30 Jun 2020 00:44:47: 8000000 INFO @ Tue, 30 Jun 2020 00:44:51: 3000000 INFO @ Tue, 30 Jun 2020 00:44:52: 9000000 INFO @ Tue, 30 Jun 2020 00:44:57: 4000000 INFO @ Tue, 30 Jun 2020 00:44:58: 10000000 INFO @ Tue, 30 Jun 2020 00:45:02: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:45:03: 11000000 INFO @ Tue, 30 Jun 2020 00:45:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:45:05: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:45:05: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:45:08: 6000000 INFO @ Tue, 30 Jun 2020 00:45:09: 12000000 INFO @ Tue, 30 Jun 2020 00:45:11: 1000000 INFO @ Tue, 30 Jun 2020 00:45:14: 7000000 INFO @ Tue, 30 Jun 2020 00:45:14: 13000000 INFO @ Tue, 30 Jun 2020 00:45:16: 2000000 INFO @ Tue, 30 Jun 2020 00:45:19: 8000000 INFO @ Tue, 30 Jun 2020 00:45:20: 14000000 INFO @ Tue, 30 Jun 2020 00:45:22: 3000000 INFO @ Tue, 30 Jun 2020 00:45:25: 9000000 INFO @ Tue, 30 Jun 2020 00:45:26: 15000000 INFO @ Tue, 30 Jun 2020 00:45:28: 4000000 INFO @ Tue, 30 Jun 2020 00:45:30: 10000000 INFO @ Tue, 30 Jun 2020 00:45:31: 16000000 INFO @ Tue, 30 Jun 2020 00:45:33: 5000000 INFO @ Tue, 30 Jun 2020 00:45:36: 11000000 INFO @ Tue, 30 Jun 2020 00:45:37: 17000000 INFO @ Tue, 30 Jun 2020 00:45:39: 6000000 INFO @ Tue, 30 Jun 2020 00:45:41: 12000000 INFO @ Tue, 30 Jun 2020 00:45:42: 18000000 INFO @ Tue, 30 Jun 2020 00:45:44: 7000000 INFO @ Tue, 30 Jun 2020 00:45:46: #1 tag size is determined as 54 bps INFO @ Tue, 30 Jun 2020 00:45:46: #1 tag size = 54 INFO @ Tue, 30 Jun 2020 00:45:46: #1 total tags in treatment: 18679460 INFO @ Tue, 30 Jun 2020 00:45:46: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:45:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:45:47: #1 tags after filtering in treatment: 18679460 INFO @ Tue, 30 Jun 2020 00:45:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:45:47: #1 finished! INFO @ Tue, 30 Jun 2020 00:45:47: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:45:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:45:47: 13000000 INFO @ Tue, 30 Jun 2020 00:45:48: #2 number of paired peaks: 95 WARNING @ Tue, 30 Jun 2020 00:45:48: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:45:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:45:50: 8000000 INFO @ Tue, 30 Jun 2020 00:45:52: 14000000 INFO @ Tue, 30 Jun 2020 00:45:55: 9000000 INFO @ Tue, 30 Jun 2020 00:45:58: 15000000 INFO @ Tue, 30 Jun 2020 00:46:01: 10000000 INFO @ Tue, 30 Jun 2020 00:46:04: 16000000 INFO @ Tue, 30 Jun 2020 00:46:06: 11000000 INFO @ Tue, 30 Jun 2020 00:46:09: 17000000 INFO @ Tue, 30 Jun 2020 00:46:12: 12000000 INFO @ Tue, 30 Jun 2020 00:46:15: 18000000 INFO @ Tue, 30 Jun 2020 00:46:18: 13000000 INFO @ Tue, 30 Jun 2020 00:46:19: #1 tag size is determined as 54 bps INFO @ Tue, 30 Jun 2020 00:46:19: #1 tag size = 54 INFO @ Tue, 30 Jun 2020 00:46:19: #1 total tags in treatment: 18679460 INFO @ Tue, 30 Jun 2020 00:46:19: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:46:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:46:19: #1 tags after filtering in treatment: 18679460 INFO @ Tue, 30 Jun 2020 00:46:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:46:19: #1 finished! INFO @ Tue, 30 Jun 2020 00:46:19: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:46:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:46:20: #2 number of paired peaks: 95 WARNING @ Tue, 30 Jun 2020 00:46:20: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:46:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:46:23: 14000000 INFO @ Tue, 30 Jun 2020 00:46:28: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:46:33: 16000000 INFO @ Tue, 30 Jun 2020 00:46:39: 17000000 INFO @ Tue, 30 Jun 2020 00:46:44: 18000000 INFO @ Tue, 30 Jun 2020 00:46:48: #1 tag size is determined as 54 bps INFO @ Tue, 30 Jun 2020 00:46:48: #1 tag size = 54 INFO @ Tue, 30 Jun 2020 00:46:48: #1 total tags in treatment: 18679460 INFO @ Tue, 30 Jun 2020 00:46:48: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:46:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:46:48: #1 tags after filtering in treatment: 18679460 INFO @ Tue, 30 Jun 2020 00:46:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:46:48: #1 finished! INFO @ Tue, 30 Jun 2020 00:46:48: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:46:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:46:49: #2 number of paired peaks: 95 WARNING @ Tue, 30 Jun 2020 00:46:49: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:46:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992690/SRX992690.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。