Job ID = 6528526 SRX = SRX992689 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:33:58 prefetch.2.10.7: 1) Downloading 'SRR1970916'... 2020-06-29T15:33:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:38:35 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:38:35 prefetch.2.10.7: 1) 'SRR1970916' was downloaded successfully Read 40401662 spots for SRR1970916/SRR1970916.sra Written 40401662 spots for SRR1970916/SRR1970916.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:11:51 40401662 reads; of these: 40401662 (100.00%) were unpaired; of these: 12909574 (31.95%) aligned 0 times 19915233 (49.29%) aligned exactly 1 time 7576855 (18.75%) aligned >1 times 68.05% overall alignment rate Time searching: 00:11:52 Overall time: 00:11:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3999123 / 27492088 = 0.1455 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:14:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:14:15: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:14:15: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:14:23: 1000000 INFO @ Tue, 30 Jun 2020 01:14:31: 2000000 INFO @ Tue, 30 Jun 2020 01:14:39: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:14:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:14:45: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:14:45: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:14:46: 4000000 INFO @ Tue, 30 Jun 2020 01:14:53: 1000000 INFO @ Tue, 30 Jun 2020 01:14:54: 5000000 INFO @ Tue, 30 Jun 2020 01:15:00: 2000000 INFO @ Tue, 30 Jun 2020 01:15:02: 6000000 INFO @ Tue, 30 Jun 2020 01:15:07: 3000000 INFO @ Tue, 30 Jun 2020 01:15:09: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:15:14: 4000000 INFO @ Tue, 30 Jun 2020 01:15:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:15:15: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:15:15: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:15:17: 8000000 INFO @ Tue, 30 Jun 2020 01:15:21: 5000000 INFO @ Tue, 30 Jun 2020 01:15:23: 1000000 INFO @ Tue, 30 Jun 2020 01:15:25: 9000000 INFO @ Tue, 30 Jun 2020 01:15:29: 6000000 INFO @ Tue, 30 Jun 2020 01:15:30: 2000000 INFO @ Tue, 30 Jun 2020 01:15:33: 10000000 INFO @ Tue, 30 Jun 2020 01:15:36: 7000000 INFO @ Tue, 30 Jun 2020 01:15:37: 3000000 INFO @ Tue, 30 Jun 2020 01:15:42: 11000000 INFO @ Tue, 30 Jun 2020 01:15:44: 8000000 INFO @ Tue, 30 Jun 2020 01:15:45: 4000000 INFO @ Tue, 30 Jun 2020 01:15:50: 12000000 INFO @ Tue, 30 Jun 2020 01:15:51: 9000000 INFO @ Tue, 30 Jun 2020 01:15:52: 5000000 INFO @ Tue, 30 Jun 2020 01:15:58: 13000000 INFO @ Tue, 30 Jun 2020 01:15:59: 10000000 INFO @ Tue, 30 Jun 2020 01:15:59: 6000000 INFO @ Tue, 30 Jun 2020 01:16:06: 11000000 INFO @ Tue, 30 Jun 2020 01:16:06: 14000000 INFO @ Tue, 30 Jun 2020 01:16:07: 7000000 INFO @ Tue, 30 Jun 2020 01:16:14: 8000000 INFO @ Tue, 30 Jun 2020 01:16:14: 12000000 INFO @ Tue, 30 Jun 2020 01:16:15: 15000000 INFO @ Tue, 30 Jun 2020 01:16:22: 13000000 INFO @ Tue, 30 Jun 2020 01:16:23: 9000000 INFO @ Tue, 30 Jun 2020 01:16:23: 16000000 INFO @ Tue, 30 Jun 2020 01:16:28: 14000000 INFO @ Tue, 30 Jun 2020 01:16:29: 10000000 INFO @ Tue, 30 Jun 2020 01:16:31: 17000000 INFO @ Tue, 30 Jun 2020 01:16:36: 15000000 INFO @ Tue, 30 Jun 2020 01:16:37: 11000000 INFO @ Tue, 30 Jun 2020 01:16:40: 18000000 INFO @ Tue, 30 Jun 2020 01:16:43: 16000000 INFO @ Tue, 30 Jun 2020 01:16:44: 12000000 INFO @ Tue, 30 Jun 2020 01:16:48: 19000000 INFO @ Tue, 30 Jun 2020 01:16:51: 17000000 INFO @ Tue, 30 Jun 2020 01:16:52: 13000000 INFO @ Tue, 30 Jun 2020 01:16:57: 20000000 INFO @ Tue, 30 Jun 2020 01:16:59: 18000000 INFO @ Tue, 30 Jun 2020 01:17:00: 14000000 INFO @ Tue, 30 Jun 2020 01:17:05: 21000000 INFO @ Tue, 30 Jun 2020 01:17:06: 19000000 INFO @ Tue, 30 Jun 2020 01:17:07: 15000000 INFO @ Tue, 30 Jun 2020 01:17:13: 22000000 INFO @ Tue, 30 Jun 2020 01:17:14: 20000000 INFO @ Tue, 30 Jun 2020 01:17:15: 16000000 INFO @ Tue, 30 Jun 2020 01:17:21: 23000000 INFO @ Tue, 30 Jun 2020 01:17:22: 21000000 INFO @ Tue, 30 Jun 2020 01:17:23: 17000000 INFO @ Tue, 30 Jun 2020 01:17:25: #1 tag size is determined as 54 bps INFO @ Tue, 30 Jun 2020 01:17:25: #1 tag size = 54 INFO @ Tue, 30 Jun 2020 01:17:25: #1 total tags in treatment: 23492965 INFO @ Tue, 30 Jun 2020 01:17:25: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:17:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:17:26: #1 tags after filtering in treatment: 23492965 INFO @ Tue, 30 Jun 2020 01:17:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:17:26: #1 finished! INFO @ Tue, 30 Jun 2020 01:17:26: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:17:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:17:27: #2 number of paired peaks: 43 WARNING @ Tue, 30 Jun 2020 01:17:27: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:17:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:17:29: 22000000 INFO @ Tue, 30 Jun 2020 01:17:30: 18000000 INFO @ Tue, 30 Jun 2020 01:17:37: 23000000 INFO @ Tue, 30 Jun 2020 01:17:38: 19000000 INFO @ Tue, 30 Jun 2020 01:17:41: #1 tag size is determined as 54 bps INFO @ Tue, 30 Jun 2020 01:17:41: #1 tag size = 54 INFO @ Tue, 30 Jun 2020 01:17:41: #1 total tags in treatment: 23492965 INFO @ Tue, 30 Jun 2020 01:17:41: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:17:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:17:41: #1 tags after filtering in treatment: 23492965 INFO @ Tue, 30 Jun 2020 01:17:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:17:41: #1 finished! INFO @ Tue, 30 Jun 2020 01:17:41: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:17:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:17:43: #2 number of paired peaks: 43 WARNING @ Tue, 30 Jun 2020 01:17:43: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:17:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:17:46: 20000000 INFO @ Tue, 30 Jun 2020 01:17:53: 21000000 INFO @ Tue, 30 Jun 2020 01:18:00: 22000000 INFO @ Tue, 30 Jun 2020 01:18:07: 23000000 INFO @ Tue, 30 Jun 2020 01:18:11: #1 tag size is determined as 54 bps INFO @ Tue, 30 Jun 2020 01:18:11: #1 tag size = 54 INFO @ Tue, 30 Jun 2020 01:18:11: #1 total tags in treatment: 23492965 INFO @ Tue, 30 Jun 2020 01:18:11: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:18:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:18:12: #1 tags after filtering in treatment: 23492965 INFO @ Tue, 30 Jun 2020 01:18:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:18:12: #1 finished! INFO @ Tue, 30 Jun 2020 01:18:12: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:18:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:18:13: #2 number of paired peaks: 43 WARNING @ Tue, 30 Jun 2020 01:18:13: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:18:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX992689/SRX992689.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。