Job ID = 9159958 sra ファイルのダウンロード中... Completed: 1770516K bytes transferred in 18 seconds (778881K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 57070259 spots for /home/okishinya/chipatlas/results/dm3/SRX992688/SRR1970915.sra Written 57070259 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:29 57070259 reads; of these: 57070259 (100.00%) were unpaired; of these: 13877655 (24.32%) aligned 0 times 31881068 (55.86%) aligned exactly 1 time 11311536 (19.82%) aligned >1 times 75.68% overall alignment rate Time searching: 00:19:30 Overall time: 00:19:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5495000 / 43192604 = 0.1272 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 01:55:57: # Command line: callpeak -t SRX992688.bam -f BAM -g dm -n SRX992688.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX992688.05 # format = BAM # ChIP-seq file = ['SRX992688.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:55:57: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:55:57: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:55:57: # Command line: callpeak -t SRX992688.bam -f BAM -g dm -n SRX992688.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX992688.10 # format = BAM # ChIP-seq file = ['SRX992688.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:55:57: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:55:57: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:55:57: # Command line: callpeak -t SRX992688.bam -f BAM -g dm -n SRX992688.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX992688.20 # format = BAM # ChIP-seq file = ['SRX992688.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:55:57: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:55:57: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:56:03: 1000000 INFO @ Wed, 28 Jun 2017 01:56:03: 1000000 INFO @ Wed, 28 Jun 2017 01:56:03: 1000000 INFO @ Wed, 28 Jun 2017 01:56:09: 2000000 INFO @ Wed, 28 Jun 2017 01:56:10: 2000000 INFO @ Wed, 28 Jun 2017 01:56:10: 2000000 INFO @ Wed, 28 Jun 2017 01:56:16: 3000000 INFO @ Wed, 28 Jun 2017 01:56:16: 3000000 INFO @ Wed, 28 Jun 2017 01:56:17: 3000000 INFO @ Wed, 28 Jun 2017 01:56:22: 4000000 INFO @ Wed, 28 Jun 2017 01:56:23: 4000000 INFO @ Wed, 28 Jun 2017 01:56:23: 4000000 INFO @ Wed, 28 Jun 2017 01:56:28: 5000000 INFO @ Wed, 28 Jun 2017 01:56:30: 5000000 INFO @ Wed, 28 Jun 2017 01:56:30: 5000000 INFO @ Wed, 28 Jun 2017 01:56:34: 6000000 INFO @ Wed, 28 Jun 2017 01:56:36: 6000000 INFO @ Wed, 28 Jun 2017 01:56:37: 6000000 INFO @ Wed, 28 Jun 2017 01:56:41: 7000000 INFO @ Wed, 28 Jun 2017 01:56:43: 7000000 INFO @ Wed, 28 Jun 2017 01:56:44: 7000000 INFO @ Wed, 28 Jun 2017 01:56:47: 8000000 INFO @ Wed, 28 Jun 2017 01:56:50: 8000000 INFO @ Wed, 28 Jun 2017 01:56:51: 8000000 INFO @ Wed, 28 Jun 2017 01:56:53: 9000000 INFO @ Wed, 28 Jun 2017 01:56:57: 9000000 INFO @ Wed, 28 Jun 2017 01:56:58: 9000000 INFO @ Wed, 28 Jun 2017 01:57:00: 10000000 INFO @ Wed, 28 Jun 2017 01:57:03: 10000000 INFO @ Wed, 28 Jun 2017 01:57:05: 10000000 INFO @ Wed, 28 Jun 2017 01:57:06: 11000000 INFO @ Wed, 28 Jun 2017 01:57:10: 11000000 INFO @ Wed, 28 Jun 2017 01:57:11: 11000000 INFO @ Wed, 28 Jun 2017 01:57:12: 12000000 INFO @ Wed, 28 Jun 2017 01:57:17: 12000000 INFO @ Wed, 28 Jun 2017 01:57:18: 12000000 INFO @ Wed, 28 Jun 2017 01:57:19: 13000000 INFO @ Wed, 28 Jun 2017 01:57:24: 13000000 INFO @ Wed, 28 Jun 2017 01:57:25: 14000000 INFO @ Wed, 28 Jun 2017 01:57:26: 13000000 INFO @ Wed, 28 Jun 2017 01:57:31: 14000000 INFO @ Wed, 28 Jun 2017 01:57:31: 15000000 INFO @ Wed, 28 Jun 2017 01:57:33: 14000000 INFO @ Wed, 28 Jun 2017 01:57:38: 15000000 INFO @ Wed, 28 Jun 2017 01:57:38: 16000000 INFO @ Wed, 28 Jun 2017 01:57:40: 15000000 INFO @ Wed, 28 Jun 2017 01:57:44: 17000000 INFO @ Wed, 28 Jun 2017 01:57:45: 16000000 INFO @ Wed, 28 Jun 2017 01:57:47: 16000000 INFO @ Wed, 28 Jun 2017 01:57:51: 18000000 INFO @ Wed, 28 Jun 2017 01:57:52: 17000000 INFO @ Wed, 28 Jun 2017 01:57:54: 17000000 INFO @ Wed, 28 Jun 2017 01:57:57: 19000000 INFO @ Wed, 28 Jun 2017 01:57:59: 18000000 INFO @ Wed, 28 Jun 2017 01:58:01: 18000000 INFO @ Wed, 28 Jun 2017 01:58:03: 20000000 INFO @ Wed, 28 Jun 2017 01:58:06: 19000000 INFO @ Wed, 28 Jun 2017 01:58:08: 19000000 INFO @ Wed, 28 Jun 2017 01:58:10: 21000000 INFO @ Wed, 28 Jun 2017 01:58:12: 20000000 INFO @ Wed, 28 Jun 2017 01:58:15: 20000000 INFO @ Wed, 28 Jun 2017 01:58:16: 22000000 INFO @ Wed, 28 Jun 2017 01:58:19: 21000000 INFO @ Wed, 28 Jun 2017 01:58:22: 21000000 INFO @ Wed, 28 Jun 2017 01:58:22: 23000000 INFO @ Wed, 28 Jun 2017 01:58:26: 22000000 INFO @ Wed, 28 Jun 2017 01:58:29: 24000000 INFO @ Wed, 28 Jun 2017 01:58:29: 22000000 INFO @ Wed, 28 Jun 2017 01:58:33: 23000000 INFO @ Wed, 28 Jun 2017 01:58:35: 25000000 INFO @ Wed, 28 Jun 2017 01:58:36: 23000000 INFO @ Wed, 28 Jun 2017 01:58:40: 24000000 INFO @ Wed, 28 Jun 2017 01:58:41: 26000000 INFO @ Wed, 28 Jun 2017 01:58:43: 24000000 INFO @ Wed, 28 Jun 2017 01:58:46: 25000000 INFO @ Wed, 28 Jun 2017 01:58:47: 27000000 INFO @ Wed, 28 Jun 2017 01:58:50: 25000000 INFO @ Wed, 28 Jun 2017 01:58:53: 26000000 INFO @ Wed, 28 Jun 2017 01:58:54: 28000000 INFO @ Wed, 28 Jun 2017 01:58:56: 26000000 INFO @ Wed, 28 Jun 2017 01:59:00: 27000000 INFO @ Wed, 28 Jun 2017 01:59:00: 29000000 INFO @ Wed, 28 Jun 2017 01:59:03: 27000000 INFO @ Wed, 28 Jun 2017 01:59:06: 28000000 INFO @ Wed, 28 Jun 2017 01:59:07: 30000000 INFO @ Wed, 28 Jun 2017 01:59:10: 28000000 INFO @ Wed, 28 Jun 2017 01:59:13: 31000000 INFO @ Wed, 28 Jun 2017 01:59:13: 29000000 INFO @ Wed, 28 Jun 2017 01:59:17: 29000000 INFO @ Wed, 28 Jun 2017 01:59:19: 32000000 INFO @ Wed, 28 Jun 2017 01:59:20: 30000000 INFO @ Wed, 28 Jun 2017 01:59:24: 30000000 INFO @ Wed, 28 Jun 2017 01:59:25: 33000000 INFO @ Wed, 28 Jun 2017 01:59:27: 31000000 INFO @ Wed, 28 Jun 2017 01:59:31: 31000000 INFO @ Wed, 28 Jun 2017 01:59:31: 34000000 INFO @ Wed, 28 Jun 2017 01:59:33: 32000000 INFO @ Wed, 28 Jun 2017 01:59:38: 35000000 INFO @ Wed, 28 Jun 2017 01:59:38: 32000000 INFO @ Wed, 28 Jun 2017 01:59:41: 33000000 INFO @ Wed, 28 Jun 2017 01:59:44: 36000000 INFO @ Wed, 28 Jun 2017 01:59:47: 33000000 INFO @ Wed, 28 Jun 2017 01:59:49: 34000000 INFO @ Wed, 28 Jun 2017 01:59:51: 37000000 INFO @ Wed, 28 Jun 2017 01:59:55: 34000000 INFO @ Wed, 28 Jun 2017 01:59:57: #1 tag size is determined as 54 bps INFO @ Wed, 28 Jun 2017 01:59:57: #1 tag size = 54 INFO @ Wed, 28 Jun 2017 01:59:57: #1 total tags in treatment: 37697604 INFO @ Wed, 28 Jun 2017 01:59:57: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:59:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:59:57: #1 tags after filtering in treatment: 37697604 INFO @ Wed, 28 Jun 2017 01:59:57: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:59:57: #1 finished! INFO @ Wed, 28 Jun 2017 01:59:57: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:59:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:59:58: 35000000 INFO @ Wed, 28 Jun 2017 02:00:00: #2 number of paired peaks: 2 WARNING @ Wed, 28 Jun 2017 02:00:00: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:00:00: Process for pairing-model is terminated! cat: SRX992688.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX992688.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX992688.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX992688.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 02:00:04: 35000000 INFO @ Wed, 28 Jun 2017 02:00:06: 36000000 INFO @ Wed, 28 Jun 2017 02:00:12: 36000000 INFO @ Wed, 28 Jun 2017 02:00:14: 37000000 INFO @ Wed, 28 Jun 2017 02:00:20: 37000000 INFO @ Wed, 28 Jun 2017 02:00:20: #1 tag size is determined as 54 bps INFO @ Wed, 28 Jun 2017 02:00:20: #1 tag size = 54 INFO @ Wed, 28 Jun 2017 02:00:20: #1 total tags in treatment: 37697604 INFO @ Wed, 28 Jun 2017 02:00:20: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:00:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:00:21: #1 tags after filtering in treatment: 37697604 INFO @ Wed, 28 Jun 2017 02:00:21: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:00:21: #1 finished! INFO @ Wed, 28 Jun 2017 02:00:21: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:00:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:00:23: #2 number of paired peaks: 2 WARNING @ Wed, 28 Jun 2017 02:00:23: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:00:23: Process for pairing-model is terminated! cat: SRX992688.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX992688.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX992688.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX992688.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 02:00:25: #1 tag size is determined as 54 bps INFO @ Wed, 28 Jun 2017 02:00:25: #1 tag size = 54 INFO @ Wed, 28 Jun 2017 02:00:25: #1 total tags in treatment: 37697604 INFO @ Wed, 28 Jun 2017 02:00:25: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:00:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:00:26: #1 tags after filtering in treatment: 37697604 INFO @ Wed, 28 Jun 2017 02:00:26: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:00:26: #1 finished! INFO @ Wed, 28 Jun 2017 02:00:26: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:00:26: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:00:28: #2 number of paired peaks: 2 WARNING @ Wed, 28 Jun 2017 02:00:28: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:00:28: Process for pairing-model is terminated! cat: SRX992688.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX992688.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX992688.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX992688.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。