Job ID = 14168773 SRX = SRX9910772 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 31848651 spots for SRR13498843/SRR13498843.sra Written 31848651 spots for SRR13498843/SRR13498843.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169843 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:12 31848651 reads; of these: 31848651 (100.00%) were unpaired; of these: 8326191 (26.14%) aligned 0 times 16639234 (52.24%) aligned exactly 1 time 6883226 (21.61%) aligned >1 times 73.86% overall alignment rate Time searching: 00:13:12 Overall time: 00:13:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6704931 / 23522460 = 0.2850 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:23:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:23:55: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:23:55: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:24:00: 1000000 INFO @ Fri, 10 Dec 2021 20:24:06: 2000000 INFO @ Fri, 10 Dec 2021 20:24:11: 3000000 INFO @ Fri, 10 Dec 2021 20:24:16: 4000000 INFO @ Fri, 10 Dec 2021 20:24:22: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:24:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:24:24: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:24:24: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:24:27: 6000000 INFO @ Fri, 10 Dec 2021 20:24:30: 1000000 INFO @ Fri, 10 Dec 2021 20:24:33: 7000000 INFO @ Fri, 10 Dec 2021 20:24:36: 2000000 INFO @ Fri, 10 Dec 2021 20:24:39: 8000000 INFO @ Fri, 10 Dec 2021 20:24:42: 3000000 INFO @ Fri, 10 Dec 2021 20:24:45: 9000000 INFO @ Fri, 10 Dec 2021 20:24:48: 4000000 INFO @ Fri, 10 Dec 2021 20:24:51: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:24:53: 5000000 INFO @ Fri, 10 Dec 2021 20:24:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:24:54: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:24:54: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:24:56: 11000000 INFO @ Fri, 10 Dec 2021 20:24:59: 6000000 INFO @ Fri, 10 Dec 2021 20:25:00: 1000000 INFO @ Fri, 10 Dec 2021 20:25:02: 12000000 INFO @ Fri, 10 Dec 2021 20:25:05: 7000000 INFO @ Fri, 10 Dec 2021 20:25:06: 2000000 INFO @ Fri, 10 Dec 2021 20:25:08: 13000000 INFO @ Fri, 10 Dec 2021 20:25:11: 8000000 INFO @ Fri, 10 Dec 2021 20:25:12: 3000000 INFO @ Fri, 10 Dec 2021 20:25:14: 14000000 INFO @ Fri, 10 Dec 2021 20:25:17: 9000000 INFO @ Fri, 10 Dec 2021 20:25:18: 4000000 INFO @ Fri, 10 Dec 2021 20:25:20: 15000000 INFO @ Fri, 10 Dec 2021 20:25:23: 10000000 INFO @ Fri, 10 Dec 2021 20:25:24: 5000000 INFO @ Fri, 10 Dec 2021 20:25:26: 16000000 INFO @ Fri, 10 Dec 2021 20:25:29: 11000000 INFO @ Fri, 10 Dec 2021 20:25:30: 6000000 INFO @ Fri, 10 Dec 2021 20:25:31: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 20:25:31: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 20:25:31: #1 total tags in treatment: 16817529 INFO @ Fri, 10 Dec 2021 20:25:31: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 20:25:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 20:25:31: #1 tags after filtering in treatment: 16817529 INFO @ Fri, 10 Dec 2021 20:25:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 20:25:31: #1 finished! INFO @ Fri, 10 Dec 2021 20:25:31: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 20:25:31: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 20:25:32: #2 number of paired peaks: 1223 INFO @ Fri, 10 Dec 2021 20:25:32: start model_add_line... INFO @ Fri, 10 Dec 2021 20:25:32: start X-correlation... INFO @ Fri, 10 Dec 2021 20:25:32: end of X-cor INFO @ Fri, 10 Dec 2021 20:25:32: #2 finished! INFO @ Fri, 10 Dec 2021 20:25:32: #2 predicted fragment length is 243 bps INFO @ Fri, 10 Dec 2021 20:25:32: #2 alternative fragment length(s) may be 243 bps INFO @ Fri, 10 Dec 2021 20:25:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.05_model.r INFO @ Fri, 10 Dec 2021 20:25:33: #3 Call peaks... INFO @ Fri, 10 Dec 2021 20:25:33: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 20:25:34: 12000000 INFO @ Fri, 10 Dec 2021 20:25:35: 7000000 INFO @ Fri, 10 Dec 2021 20:25:40: 13000000 INFO @ Fri, 10 Dec 2021 20:25:41: 8000000 INFO @ Fri, 10 Dec 2021 20:25:46: 14000000 INFO @ Fri, 10 Dec 2021 20:25:47: 9000000 INFO @ Fri, 10 Dec 2021 20:25:52: 15000000 INFO @ Fri, 10 Dec 2021 20:25:53: 10000000 INFO @ Fri, 10 Dec 2021 20:25:58: 16000000 INFO @ Fri, 10 Dec 2021 20:25:59: 11000000 INFO @ Fri, 10 Dec 2021 20:26:03: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 20:26:03: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 20:26:03: #1 total tags in treatment: 16817529 INFO @ Fri, 10 Dec 2021 20:26:03: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 20:26:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 20:26:03: #1 tags after filtering in treatment: 16817529 INFO @ Fri, 10 Dec 2021 20:26:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 20:26:03: #1 finished! INFO @ Fri, 10 Dec 2021 20:26:03: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 20:26:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 20:26:04: #2 number of paired peaks: 1223 INFO @ Fri, 10 Dec 2021 20:26:04: start model_add_line... INFO @ Fri, 10 Dec 2021 20:26:04: 12000000 INFO @ Fri, 10 Dec 2021 20:26:04: start X-correlation... INFO @ Fri, 10 Dec 2021 20:26:04: end of X-cor INFO @ Fri, 10 Dec 2021 20:26:04: #2 finished! INFO @ Fri, 10 Dec 2021 20:26:04: #2 predicted fragment length is 243 bps INFO @ Fri, 10 Dec 2021 20:26:04: #2 alternative fragment length(s) may be 243 bps INFO @ Fri, 10 Dec 2021 20:26:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.10_model.r INFO @ Fri, 10 Dec 2021 20:26:04: #3 Call peaks... INFO @ Fri, 10 Dec 2021 20:26:04: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 20:26:09: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 20:26:10: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 20:26:15: 14000000 INFO @ Fri, 10 Dec 2021 20:26:21: 15000000 INFO @ Fri, 10 Dec 2021 20:26:26: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.05_peaks.xls INFO @ Fri, 10 Dec 2021 20:26:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 20:26:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.05_summits.bed INFO @ Fri, 10 Dec 2021 20:26:26: Done! INFO @ Fri, 10 Dec 2021 20:26:26: 16000000 pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (6386 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 20:26:31: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 20:26:31: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 20:26:31: #1 total tags in treatment: 16817529 INFO @ Fri, 10 Dec 2021 20:26:31: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 20:26:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 20:26:31: #1 tags after filtering in treatment: 16817529 INFO @ Fri, 10 Dec 2021 20:26:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 20:26:31: #1 finished! INFO @ Fri, 10 Dec 2021 20:26:31: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 20:26:31: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 20:26:32: #2 number of paired peaks: 1223 INFO @ Fri, 10 Dec 2021 20:26:32: start model_add_line... INFO @ Fri, 10 Dec 2021 20:26:33: start X-correlation... INFO @ Fri, 10 Dec 2021 20:26:33: end of X-cor INFO @ Fri, 10 Dec 2021 20:26:33: #2 finished! INFO @ Fri, 10 Dec 2021 20:26:33: #2 predicted fragment length is 243 bps INFO @ Fri, 10 Dec 2021 20:26:33: #2 alternative fragment length(s) may be 243 bps INFO @ Fri, 10 Dec 2021 20:26:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.20_model.r INFO @ Fri, 10 Dec 2021 20:26:33: #3 Call peaks... INFO @ Fri, 10 Dec 2021 20:26:33: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 20:26:41: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 20:26:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.10_peaks.xls INFO @ Fri, 10 Dec 2021 20:26:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 20:26:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.10_summits.bed INFO @ Fri, 10 Dec 2021 20:26:58: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (4715 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 20:27:09: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 20:27:26: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.20_peaks.xls INFO @ Fri, 10 Dec 2021 20:27:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 20:27:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9910772/SRX9910772.20_summits.bed INFO @ Fri, 10 Dec 2021 20:27:26: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (3206 records, 4 fields): 5 millis CompletedMACS2peakCalling