Job ID = 14172150 SRX = SRX9891436 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25900388 spots for SRR13478592/SRR13478592.sra Written 25900388 spots for SRR13478592/SRR13478592.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172615 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:16 25900388 reads; of these: 25900388 (100.00%) were unpaired; of these: 6200607 (23.94%) aligned 0 times 16708823 (64.51%) aligned exactly 1 time 2990958 (11.55%) aligned >1 times 76.06% overall alignment rate Time searching: 00:06:16 Overall time: 00:06:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2932978 / 19699781 = 0.1489 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:43:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:43:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:43:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:43:24: 1000000 INFO @ Sat, 11 Dec 2021 14:43:30: 2000000 INFO @ Sat, 11 Dec 2021 14:43:35: 3000000 INFO @ Sat, 11 Dec 2021 14:43:41: 4000000 INFO @ Sat, 11 Dec 2021 14:43:46: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:43:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:43:49: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:43:49: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:43:51: 6000000 INFO @ Sat, 11 Dec 2021 14:43:55: 1000000 INFO @ Sat, 11 Dec 2021 14:43:57: 7000000 INFO @ Sat, 11 Dec 2021 14:44:00: 2000000 INFO @ Sat, 11 Dec 2021 14:44:03: 8000000 INFO @ Sat, 11 Dec 2021 14:44:06: 3000000 INFO @ Sat, 11 Dec 2021 14:44:09: 9000000 INFO @ Sat, 11 Dec 2021 14:44:12: 4000000 INFO @ Sat, 11 Dec 2021 14:44:14: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:44:18: 5000000 INFO @ Sat, 11 Dec 2021 14:44:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:44:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:44:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:44:20: 11000000 INFO @ Sat, 11 Dec 2021 14:44:23: 6000000 INFO @ Sat, 11 Dec 2021 14:44:25: 1000000 INFO @ Sat, 11 Dec 2021 14:44:26: 12000000 INFO @ Sat, 11 Dec 2021 14:44:29: 7000000 INFO @ Sat, 11 Dec 2021 14:44:32: 13000000 INFO @ Sat, 11 Dec 2021 14:44:32: 2000000 INFO @ Sat, 11 Dec 2021 14:44:35: 8000000 INFO @ Sat, 11 Dec 2021 14:44:38: 14000000 INFO @ Sat, 11 Dec 2021 14:44:39: 3000000 INFO @ Sat, 11 Dec 2021 14:44:41: 9000000 INFO @ Sat, 11 Dec 2021 14:44:44: 15000000 INFO @ Sat, 11 Dec 2021 14:44:45: 4000000 INFO @ Sat, 11 Dec 2021 14:44:47: 10000000 INFO @ Sat, 11 Dec 2021 14:44:50: 16000000 INFO @ Sat, 11 Dec 2021 14:44:52: 5000000 INFO @ Sat, 11 Dec 2021 14:44:53: 11000000 INFO @ Sat, 11 Dec 2021 14:44:54: #1 tag size is determined as 60 bps INFO @ Sat, 11 Dec 2021 14:44:54: #1 tag size = 60 INFO @ Sat, 11 Dec 2021 14:44:54: #1 total tags in treatment: 16766803 INFO @ Sat, 11 Dec 2021 14:44:54: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:44:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:44:55: #1 tags after filtering in treatment: 16766803 INFO @ Sat, 11 Dec 2021 14:44:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:44:55: #1 finished! INFO @ Sat, 11 Dec 2021 14:44:55: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:44:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:44:56: #2 number of paired peaks: 9 WARNING @ Sat, 11 Dec 2021 14:44:56: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:44:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 14:44:58: 6000000 INFO @ Sat, 11 Dec 2021 14:44:59: 12000000 INFO @ Sat, 11 Dec 2021 14:45:05: 7000000 INFO @ Sat, 11 Dec 2021 14:45:05: 13000000 INFO @ Sat, 11 Dec 2021 14:45:11: 14000000 INFO @ Sat, 11 Dec 2021 14:45:11: 8000000 INFO @ Sat, 11 Dec 2021 14:45:17: 15000000 INFO @ Sat, 11 Dec 2021 14:45:18: 9000000 INFO @ Sat, 11 Dec 2021 14:45:23: 16000000 INFO @ Sat, 11 Dec 2021 14:45:25: 10000000 INFO @ Sat, 11 Dec 2021 14:45:28: #1 tag size is determined as 60 bps INFO @ Sat, 11 Dec 2021 14:45:28: #1 tag size = 60 INFO @ Sat, 11 Dec 2021 14:45:28: #1 total tags in treatment: 16766803 INFO @ Sat, 11 Dec 2021 14:45:28: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:45:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:45:28: #1 tags after filtering in treatment: 16766803 INFO @ Sat, 11 Dec 2021 14:45:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:45:28: #1 finished! INFO @ Sat, 11 Dec 2021 14:45:28: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:45:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:45:29: #2 number of paired peaks: 9 WARNING @ Sat, 11 Dec 2021 14:45:29: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:45:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 14:45:31: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 14:45:37: 12000000 INFO @ Sat, 11 Dec 2021 14:45:44: 13000000 INFO @ Sat, 11 Dec 2021 14:45:50: 14000000 INFO @ Sat, 11 Dec 2021 14:45:56: 15000000 INFO @ Sat, 11 Dec 2021 14:46:03: 16000000 INFO @ Sat, 11 Dec 2021 14:46:08: #1 tag size is determined as 60 bps INFO @ Sat, 11 Dec 2021 14:46:08: #1 tag size = 60 INFO @ Sat, 11 Dec 2021 14:46:08: #1 total tags in treatment: 16766803 INFO @ Sat, 11 Dec 2021 14:46:08: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:46:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:46:08: #1 tags after filtering in treatment: 16766803 INFO @ Sat, 11 Dec 2021 14:46:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:46:08: #1 finished! INFO @ Sat, 11 Dec 2021 14:46:08: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:46:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:46:09: #2 number of paired peaks: 9 WARNING @ Sat, 11 Dec 2021 14:46:09: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:46:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891436/SRX9891436.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。