Job ID = 14172146 SRX = SRX9891432 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 26387552 spots for SRR13478588/SRR13478588.sra Written 26387552 spots for SRR13478588/SRR13478588.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172614 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:24 26387552 reads; of these: 26387552 (100.00%) were unpaired; of these: 1583454 (6.00%) aligned 0 times 21775002 (82.52%) aligned exactly 1 time 3029096 (11.48%) aligned >1 times 94.00% overall alignment rate Time searching: 00:07:24 Overall time: 00:07:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2759665 / 24804098 = 0.1113 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:44:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:44:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:44:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:44:25: 1000000 INFO @ Sat, 11 Dec 2021 14:44:31: 2000000 INFO @ Sat, 11 Dec 2021 14:44:37: 3000000 INFO @ Sat, 11 Dec 2021 14:44:43: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:44:49: 5000000 INFO @ Sat, 11 Dec 2021 14:44:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:44:49: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:44:49: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:44:55: 6000000 INFO @ Sat, 11 Dec 2021 14:44:56: 1000000 INFO @ Sat, 11 Dec 2021 14:45:01: 7000000 INFO @ Sat, 11 Dec 2021 14:45:03: 2000000 INFO @ Sat, 11 Dec 2021 14:45:07: 8000000 INFO @ Sat, 11 Dec 2021 14:45:10: 3000000 INFO @ Sat, 11 Dec 2021 14:45:13: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:45:17: 4000000 INFO @ Sat, 11 Dec 2021 14:45:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:45:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:45:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:45:19: 10000000 INFO @ Sat, 11 Dec 2021 14:45:24: 5000000 INFO @ Sat, 11 Dec 2021 14:45:26: 1000000 INFO @ Sat, 11 Dec 2021 14:45:26: 11000000 INFO @ Sat, 11 Dec 2021 14:45:32: 6000000 INFO @ Sat, 11 Dec 2021 14:45:32: 2000000 INFO @ Sat, 11 Dec 2021 14:45:32: 12000000 INFO @ Sat, 11 Dec 2021 14:45:38: 3000000 INFO @ Sat, 11 Dec 2021 14:45:38: 13000000 INFO @ Sat, 11 Dec 2021 14:45:39: 7000000 INFO @ Sat, 11 Dec 2021 14:45:44: 14000000 INFO @ Sat, 11 Dec 2021 14:45:44: 4000000 INFO @ Sat, 11 Dec 2021 14:45:46: 8000000 INFO @ Sat, 11 Dec 2021 14:45:51: 15000000 INFO @ Sat, 11 Dec 2021 14:45:51: 5000000 INFO @ Sat, 11 Dec 2021 14:45:53: 9000000 INFO @ Sat, 11 Dec 2021 14:45:57: 16000000 INFO @ Sat, 11 Dec 2021 14:45:57: 6000000 INFO @ Sat, 11 Dec 2021 14:46:00: 10000000 INFO @ Sat, 11 Dec 2021 14:46:03: 17000000 INFO @ Sat, 11 Dec 2021 14:46:03: 7000000 INFO @ Sat, 11 Dec 2021 14:46:08: 11000000 INFO @ Sat, 11 Dec 2021 14:46:10: 18000000 INFO @ Sat, 11 Dec 2021 14:46:10: 8000000 INFO @ Sat, 11 Dec 2021 14:46:15: 12000000 INFO @ Sat, 11 Dec 2021 14:46:16: 19000000 INFO @ Sat, 11 Dec 2021 14:46:16: 9000000 INFO @ Sat, 11 Dec 2021 14:46:22: 13000000 INFO @ Sat, 11 Dec 2021 14:46:22: 20000000 INFO @ Sat, 11 Dec 2021 14:46:22: 10000000 INFO @ Sat, 11 Dec 2021 14:46:29: 21000000 INFO @ Sat, 11 Dec 2021 14:46:29: 11000000 INFO @ Sat, 11 Dec 2021 14:46:29: 14000000 INFO @ Sat, 11 Dec 2021 14:46:35: 22000000 INFO @ Sat, 11 Dec 2021 14:46:35: 12000000 INFO @ Sat, 11 Dec 2021 14:46:35: #1 tag size is determined as 60 bps INFO @ Sat, 11 Dec 2021 14:46:35: #1 tag size = 60 INFO @ Sat, 11 Dec 2021 14:46:35: #1 total tags in treatment: 22044433 INFO @ Sat, 11 Dec 2021 14:46:35: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:46:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:46:36: #1 tags after filtering in treatment: 22044433 INFO @ Sat, 11 Dec 2021 14:46:36: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:46:36: #1 finished! INFO @ Sat, 11 Dec 2021 14:46:36: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:46:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:46:37: 15000000 INFO @ Sat, 11 Dec 2021 14:46:37: #2 number of paired peaks: 1 WARNING @ Sat, 11 Dec 2021 14:46:37: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:46:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 14:46:41: 13000000 INFO @ Sat, 11 Dec 2021 14:46:44: 16000000 INFO @ Sat, 11 Dec 2021 14:46:47: 14000000 INFO @ Sat, 11 Dec 2021 14:46:51: 17000000 INFO @ Sat, 11 Dec 2021 14:46:54: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 14:46:59: 18000000 INFO @ Sat, 11 Dec 2021 14:47:00: 16000000 INFO @ Sat, 11 Dec 2021 14:47:06: 19000000 INFO @ Sat, 11 Dec 2021 14:47:06: 17000000 INFO @ Sat, 11 Dec 2021 14:47:13: 18000000 INFO @ Sat, 11 Dec 2021 14:47:14: 20000000 INFO @ Sat, 11 Dec 2021 14:47:19: 19000000 INFO @ Sat, 11 Dec 2021 14:47:21: 21000000 INFO @ Sat, 11 Dec 2021 14:47:26: 20000000 INFO @ Sat, 11 Dec 2021 14:47:28: 22000000 INFO @ Sat, 11 Dec 2021 14:47:29: #1 tag size is determined as 60 bps INFO @ Sat, 11 Dec 2021 14:47:29: #1 tag size = 60 INFO @ Sat, 11 Dec 2021 14:47:29: #1 total tags in treatment: 22044433 INFO @ Sat, 11 Dec 2021 14:47:29: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:47:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:47:29: #1 tags after filtering in treatment: 22044433 INFO @ Sat, 11 Dec 2021 14:47:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:47:29: #1 finished! INFO @ Sat, 11 Dec 2021 14:47:29: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:47:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:47:31: #2 number of paired peaks: 1 WARNING @ Sat, 11 Dec 2021 14:47:31: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:47:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 14:47:32: 21000000 INFO @ Sat, 11 Dec 2021 14:47:37: 22000000 INFO @ Sat, 11 Dec 2021 14:47:38: #1 tag size is determined as 60 bps INFO @ Sat, 11 Dec 2021 14:47:38: #1 tag size = 60 INFO @ Sat, 11 Dec 2021 14:47:38: #1 total tags in treatment: 22044433 INFO @ Sat, 11 Dec 2021 14:47:38: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:47:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:47:38: #1 tags after filtering in treatment: 22044433 INFO @ Sat, 11 Dec 2021 14:47:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:47:38: #1 finished! INFO @ Sat, 11 Dec 2021 14:47:38: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:47:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:47:40: #2 number of paired peaks: 1 WARNING @ Sat, 11 Dec 2021 14:47:40: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:47:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9891432/SRX9891432.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。