Job ID = 14167024 SRX = SRX9717951 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 54090415 spots for SRR13288858/SRR13288858.sra Written 54090415 spots for SRR13288858/SRR13288858.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167400 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:04 54090415 reads; of these: 54090415 (100.00%) were unpaired; of these: 13638655 (25.21%) aligned 0 times 29247398 (54.07%) aligned exactly 1 time 11204362 (20.71%) aligned >1 times 74.79% overall alignment rate Time searching: 00:18:04 Overall time: 00:18:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 18656036 / 40451760 = 0.4612 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:19:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:19:50: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:19:50: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:19:55: 1000000 INFO @ Fri, 10 Dec 2021 09:20:00: 2000000 INFO @ Fri, 10 Dec 2021 09:20:05: 3000000 INFO @ Fri, 10 Dec 2021 09:20:10: 4000000 INFO @ Fri, 10 Dec 2021 09:20:16: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:20:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:20:19: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:20:19: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:20:21: 6000000 INFO @ Fri, 10 Dec 2021 09:20:24: 1000000 INFO @ Fri, 10 Dec 2021 09:20:26: 7000000 INFO @ Fri, 10 Dec 2021 09:20:28: 2000000 INFO @ Fri, 10 Dec 2021 09:20:31: 8000000 INFO @ Fri, 10 Dec 2021 09:20:33: 3000000 INFO @ Fri, 10 Dec 2021 09:20:36: 9000000 INFO @ Fri, 10 Dec 2021 09:20:38: 4000000 INFO @ Fri, 10 Dec 2021 09:20:41: 10000000 INFO @ Fri, 10 Dec 2021 09:20:42: 5000000 INFO @ Fri, 10 Dec 2021 09:20:47: 11000000 INFO @ Fri, 10 Dec 2021 09:20:47: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:20:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:20:49: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:20:49: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:20:51: 7000000 INFO @ Fri, 10 Dec 2021 09:20:52: 12000000 INFO @ Fri, 10 Dec 2021 09:20:54: 1000000 INFO @ Fri, 10 Dec 2021 09:20:56: 8000000 INFO @ Fri, 10 Dec 2021 09:20:57: 13000000 INFO @ Fri, 10 Dec 2021 09:20:58: 2000000 INFO @ Fri, 10 Dec 2021 09:21:01: 9000000 INFO @ Fri, 10 Dec 2021 09:21:02: 14000000 INFO @ Fri, 10 Dec 2021 09:21:03: 3000000 INFO @ Fri, 10 Dec 2021 09:21:05: 10000000 INFO @ Fri, 10 Dec 2021 09:21:07: 15000000 INFO @ Fri, 10 Dec 2021 09:21:08: 4000000 INFO @ Fri, 10 Dec 2021 09:21:10: 11000000 INFO @ Fri, 10 Dec 2021 09:21:12: 5000000 INFO @ Fri, 10 Dec 2021 09:21:12: 16000000 INFO @ Fri, 10 Dec 2021 09:21:14: 12000000 INFO @ Fri, 10 Dec 2021 09:21:17: 6000000 INFO @ Fri, 10 Dec 2021 09:21:18: 17000000 INFO @ Fri, 10 Dec 2021 09:21:19: 13000000 INFO @ Fri, 10 Dec 2021 09:21:22: 7000000 INFO @ Fri, 10 Dec 2021 09:21:23: 18000000 INFO @ Fri, 10 Dec 2021 09:21:23: 14000000 INFO @ Fri, 10 Dec 2021 09:21:26: 8000000 INFO @ Fri, 10 Dec 2021 09:21:28: 19000000 INFO @ Fri, 10 Dec 2021 09:21:28: 15000000 INFO @ Fri, 10 Dec 2021 09:21:31: 9000000 INFO @ Fri, 10 Dec 2021 09:21:33: 16000000 INFO @ Fri, 10 Dec 2021 09:21:33: 20000000 INFO @ Fri, 10 Dec 2021 09:21:35: 10000000 INFO @ Fri, 10 Dec 2021 09:21:37: 17000000 INFO @ Fri, 10 Dec 2021 09:21:38: 21000000 INFO @ Fri, 10 Dec 2021 09:21:40: 11000000 INFO @ Fri, 10 Dec 2021 09:21:42: 18000000 INFO @ Fri, 10 Dec 2021 09:21:43: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:21:43: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:21:43: #1 total tags in treatment: 21795724 INFO @ Fri, 10 Dec 2021 09:21:43: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:21:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:21:43: #1 tags after filtering in treatment: 21795724 INFO @ Fri, 10 Dec 2021 09:21:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:21:43: #1 finished! INFO @ Fri, 10 Dec 2021 09:21:43: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:21:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:21:44: #2 number of paired peaks: 6 WARNING @ Fri, 10 Dec 2021 09:21:44: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:21:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:21:45: 12000000 INFO @ Fri, 10 Dec 2021 09:21:46: 19000000 INFO @ Fri, 10 Dec 2021 09:21:49: 13000000 INFO @ Fri, 10 Dec 2021 09:21:51: 20000000 INFO @ Fri, 10 Dec 2021 09:21:54: 14000000 INFO @ Fri, 10 Dec 2021 09:21:55: 21000000 INFO @ Fri, 10 Dec 2021 09:21:58: 15000000 INFO @ Fri, 10 Dec 2021 09:21:59: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:21:59: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:21:59: #1 total tags in treatment: 21795724 INFO @ Fri, 10 Dec 2021 09:21:59: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:21:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:22:00: #1 tags after filtering in treatment: 21795724 INFO @ Fri, 10 Dec 2021 09:22:00: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:22:00: #1 finished! INFO @ Fri, 10 Dec 2021 09:22:00: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:22:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:22:01: #2 number of paired peaks: 6 WARNING @ Fri, 10 Dec 2021 09:22:01: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:22:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:22:03: 16000000 INFO @ Fri, 10 Dec 2021 09:22:07: 17000000 INFO @ Fri, 10 Dec 2021 09:22:11: 18000000 INFO @ Fri, 10 Dec 2021 09:22:16: 19000000 INFO @ Fri, 10 Dec 2021 09:22:20: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 09:22:25: 21000000 INFO @ Fri, 10 Dec 2021 09:22:28: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:22:28: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:22:28: #1 total tags in treatment: 21795724 INFO @ Fri, 10 Dec 2021 09:22:28: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:22:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:22:29: #1 tags after filtering in treatment: 21795724 INFO @ Fri, 10 Dec 2021 09:22:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:22:29: #1 finished! INFO @ Fri, 10 Dec 2021 09:22:29: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:22:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:22:30: #2 number of paired peaks: 6 WARNING @ Fri, 10 Dec 2021 09:22:30: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:22:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717951/SRX9717951.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。