Job ID = 14167015 SRX = SRX9717949 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 65978093 spots for SRR13288856/SRR13288856.sra Written 65978093 spots for SRR13288856/SRR13288856.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167412 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:29:17 65978093 reads; of these: 65978093 (100.00%) were unpaired; of these: 23266613 (35.26%) aligned 0 times 31344818 (47.51%) aligned exactly 1 time 11366662 (17.23%) aligned >1 times 64.74% overall alignment rate Time searching: 00:29:18 Overall time: 00:29:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 27841714 / 42711480 = 0.6519 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:34:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:34:16: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:34:16: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:34:26: 1000000 INFO @ Fri, 10 Dec 2021 09:34:36: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:34:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:34:45: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:34:45: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:34:45: 3000000 INFO @ Fri, 10 Dec 2021 09:34:52: 1000000 INFO @ Fri, 10 Dec 2021 09:34:54: 4000000 INFO @ Fri, 10 Dec 2021 09:34:59: 2000000 INFO @ Fri, 10 Dec 2021 09:35:04: 5000000 INFO @ Fri, 10 Dec 2021 09:35:07: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:35:14: 6000000 INFO @ Fri, 10 Dec 2021 09:35:15: 4000000 INFO @ Fri, 10 Dec 2021 09:35:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:35:15: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:35:15: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:35:22: 5000000 INFO @ Fri, 10 Dec 2021 09:35:23: 1000000 INFO @ Fri, 10 Dec 2021 09:35:24: 7000000 INFO @ Fri, 10 Dec 2021 09:35:30: 6000000 INFO @ Fri, 10 Dec 2021 09:35:30: 2000000 INFO @ Fri, 10 Dec 2021 09:35:33: 8000000 INFO @ Fri, 10 Dec 2021 09:35:37: 7000000 INFO @ Fri, 10 Dec 2021 09:35:38: 3000000 INFO @ Fri, 10 Dec 2021 09:35:43: 9000000 INFO @ Fri, 10 Dec 2021 09:35:45: 8000000 INFO @ Fri, 10 Dec 2021 09:35:46: 4000000 INFO @ Fri, 10 Dec 2021 09:35:53: 10000000 INFO @ Fri, 10 Dec 2021 09:35:54: 9000000 INFO @ Fri, 10 Dec 2021 09:35:55: 5000000 INFO @ Fri, 10 Dec 2021 09:36:02: 10000000 INFO @ Fri, 10 Dec 2021 09:36:03: 11000000 INFO @ Fri, 10 Dec 2021 09:36:03: 6000000 INFO @ Fri, 10 Dec 2021 09:36:10: 11000000 INFO @ Fri, 10 Dec 2021 09:36:11: 7000000 INFO @ Fri, 10 Dec 2021 09:36:12: 12000000 INFO @ Fri, 10 Dec 2021 09:36:18: 12000000 INFO @ Fri, 10 Dec 2021 09:36:20: 8000000 INFO @ Fri, 10 Dec 2021 09:36:22: 13000000 INFO @ Fri, 10 Dec 2021 09:36:25: 13000000 INFO @ Fri, 10 Dec 2021 09:36:27: 9000000 INFO @ Fri, 10 Dec 2021 09:36:32: 14000000 INFO @ Fri, 10 Dec 2021 09:36:33: 14000000 INFO @ Fri, 10 Dec 2021 09:36:35: 10000000 INFO @ Fri, 10 Dec 2021 09:36:40: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:36:40: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:36:40: #1 total tags in treatment: 14869766 INFO @ Fri, 10 Dec 2021 09:36:40: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:36:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:36:40: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:36:40: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:36:40: #1 total tags in treatment: 14869766 INFO @ Fri, 10 Dec 2021 09:36:40: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:36:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:36:41: #1 tags after filtering in treatment: 14869766 INFO @ Fri, 10 Dec 2021 09:36:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:36:41: #1 finished! INFO @ Fri, 10 Dec 2021 09:36:41: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:36:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:36:41: #1 tags after filtering in treatment: 14869766 INFO @ Fri, 10 Dec 2021 09:36:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:36:41: #1 finished! INFO @ Fri, 10 Dec 2021 09:36:41: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:36:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:36:42: #2 number of paired peaks: 47 WARNING @ Fri, 10 Dec 2021 09:36:42: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:36:42: Process for pairing-model is terminated! INFO @ Fri, 10 Dec 2021 09:36:42: #2 number of paired peaks: 47 WARNING @ Fri, 10 Dec 2021 09:36:42: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:36:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) cut: /home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.10_peaks.narrowPeak: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:36:43: 11000000 INFO @ Fri, 10 Dec 2021 09:36:50: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 09:36:58: 13000000 INFO @ Fri, 10 Dec 2021 09:37:06: 14000000 INFO @ Fri, 10 Dec 2021 09:37:13: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:37:13: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:37:13: #1 total tags in treatment: 14869766 INFO @ Fri, 10 Dec 2021 09:37:13: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:37:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:37:13: #1 tags after filtering in treatment: 14869766 INFO @ Fri, 10 Dec 2021 09:37:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:37:13: #1 finished! INFO @ Fri, 10 Dec 2021 09:37:13: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:37:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:37:14: #2 number of paired peaks: 47 WARNING @ Fri, 10 Dec 2021 09:37:14: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:37:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717949/SRX9717949.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。