Job ID = 14171400 SRX = SRX9711626 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16630224 spots for SRR13282241/SRR13282241.sra Written 16630224 spots for SRR13282241/SRR13282241.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171998 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:14 16630224 reads; of these: 16630224 (100.00%) were paired; of these: 4049959 (24.35%) aligned concordantly 0 times 10338129 (62.16%) aligned concordantly exactly 1 time 2242136 (13.48%) aligned concordantly >1 times ---- 4049959 pairs aligned concordantly 0 times; of these: 1461144 (36.08%) aligned discordantly 1 time ---- 2588815 pairs aligned 0 times concordantly or discordantly; of these: 5177630 mates make up the pairs; of these: 4199483 (81.11%) aligned 0 times 423574 (8.18%) aligned exactly 1 time 554573 (10.71%) aligned >1 times 87.37% overall alignment rate Time searching: 00:25:14 Overall time: 00:25:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 336163 / 13926400 = 0.0241 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:07:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:07:39: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:07:39: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:07:47: 1000000 INFO @ Sat, 11 Dec 2021 12:07:55: 2000000 INFO @ Sat, 11 Dec 2021 12:08:04: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:08:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:08:08: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:08:08: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:08:13: 4000000 INFO @ Sat, 11 Dec 2021 12:08:17: 1000000 INFO @ Sat, 11 Dec 2021 12:08:22: 5000000 INFO @ Sat, 11 Dec 2021 12:08:25: 2000000 INFO @ Sat, 11 Dec 2021 12:08:32: 6000000 INFO @ Sat, 11 Dec 2021 12:08:33: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:08:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:08:38: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:08:38: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:08:41: 4000000 INFO @ Sat, 11 Dec 2021 12:08:41: 7000000 INFO @ Sat, 11 Dec 2021 12:08:47: 1000000 INFO @ Sat, 11 Dec 2021 12:08:49: 5000000 INFO @ Sat, 11 Dec 2021 12:08:51: 8000000 INFO @ Sat, 11 Dec 2021 12:08:55: 2000000 INFO @ Sat, 11 Dec 2021 12:08:58: 6000000 INFO @ Sat, 11 Dec 2021 12:09:01: 9000000 INFO @ Sat, 11 Dec 2021 12:09:04: 3000000 INFO @ Sat, 11 Dec 2021 12:09:07: 7000000 INFO @ Sat, 11 Dec 2021 12:09:10: 10000000 INFO @ Sat, 11 Dec 2021 12:09:12: 4000000 INFO @ Sat, 11 Dec 2021 12:09:15: 8000000 INFO @ Sat, 11 Dec 2021 12:09:19: 11000000 INFO @ Sat, 11 Dec 2021 12:09:21: 5000000 INFO @ Sat, 11 Dec 2021 12:09:24: 9000000 INFO @ Sat, 11 Dec 2021 12:09:29: 12000000 INFO @ Sat, 11 Dec 2021 12:09:29: 6000000 INFO @ Sat, 11 Dec 2021 12:09:32: 10000000 INFO @ Sat, 11 Dec 2021 12:09:38: 7000000 INFO @ Sat, 11 Dec 2021 12:09:38: 13000000 INFO @ Sat, 11 Dec 2021 12:09:41: 11000000 INFO @ Sat, 11 Dec 2021 12:09:46: 8000000 INFO @ Sat, 11 Dec 2021 12:09:48: 14000000 INFO @ Sat, 11 Dec 2021 12:09:49: 12000000 INFO @ Sat, 11 Dec 2021 12:09:55: 9000000 INFO @ Sat, 11 Dec 2021 12:09:57: 15000000 INFO @ Sat, 11 Dec 2021 12:09:57: 13000000 INFO @ Sat, 11 Dec 2021 12:10:03: 10000000 INFO @ Sat, 11 Dec 2021 12:10:06: 14000000 INFO @ Sat, 11 Dec 2021 12:10:06: 16000000 INFO @ Sat, 11 Dec 2021 12:10:12: 11000000 INFO @ Sat, 11 Dec 2021 12:10:14: 15000000 INFO @ Sat, 11 Dec 2021 12:10:16: 17000000 INFO @ Sat, 11 Dec 2021 12:10:20: 12000000 INFO @ Sat, 11 Dec 2021 12:10:23: 16000000 INFO @ Sat, 11 Dec 2021 12:10:25: 18000000 INFO @ Sat, 11 Dec 2021 12:10:29: 13000000 INFO @ Sat, 11 Dec 2021 12:10:31: 17000000 INFO @ Sat, 11 Dec 2021 12:10:34: 19000000 INFO @ Sat, 11 Dec 2021 12:10:37: 14000000 INFO @ Sat, 11 Dec 2021 12:10:40: 18000000 INFO @ Sat, 11 Dec 2021 12:10:44: 20000000 INFO @ Sat, 11 Dec 2021 12:10:46: 15000000 INFO @ Sat, 11 Dec 2021 12:10:48: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 12:10:53: 21000000 INFO @ Sat, 11 Dec 2021 12:10:54: 16000000 INFO @ Sat, 11 Dec 2021 12:10:56: 20000000 INFO @ Sat, 11 Dec 2021 12:11:02: 22000000 INFO @ Sat, 11 Dec 2021 12:11:02: 17000000 INFO @ Sat, 11 Dec 2021 12:11:05: 21000000 INFO @ Sat, 11 Dec 2021 12:11:11: 18000000 INFO @ Sat, 11 Dec 2021 12:11:12: 23000000 INFO @ Sat, 11 Dec 2021 12:11:13: 22000000 INFO @ Sat, 11 Dec 2021 12:11:19: 19000000 INFO @ Sat, 11 Dec 2021 12:11:21: 24000000 INFO @ Sat, 11 Dec 2021 12:11:21: 23000000 INFO @ Sat, 11 Dec 2021 12:11:27: 20000000 INFO @ Sat, 11 Dec 2021 12:11:30: 24000000 INFO @ Sat, 11 Dec 2021 12:11:30: 25000000 INFO @ Sat, 11 Dec 2021 12:11:36: 21000000 INFO @ Sat, 11 Dec 2021 12:11:38: 25000000 INFO @ Sat, 11 Dec 2021 12:11:40: 26000000 INFO @ Sat, 11 Dec 2021 12:11:44: 22000000 INFO @ Sat, 11 Dec 2021 12:11:46: 26000000 INFO @ Sat, 11 Dec 2021 12:11:49: 27000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 12:11:52: 23000000 INFO @ Sat, 11 Dec 2021 12:11:55: 27000000 INFO @ Sat, 11 Dec 2021 12:11:59: 28000000 INFO @ Sat, 11 Dec 2021 12:12:01: 24000000 INFO @ Sat, 11 Dec 2021 12:12:02: #1 tag size is determined as 101 bps INFO @ Sat, 11 Dec 2021 12:12:02: #1 tag size = 101 INFO @ Sat, 11 Dec 2021 12:12:02: #1 total tags in treatment: 12269181 INFO @ Sat, 11 Dec 2021 12:12:02: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:12:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:12:02: #1 tags after filtering in treatment: 11229819 INFO @ Sat, 11 Dec 2021 12:12:02: #1 Redundant rate of treatment: 0.08 INFO @ Sat, 11 Dec 2021 12:12:02: #1 finished! INFO @ Sat, 11 Dec 2021 12:12:02: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:12:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:12:03: 28000000 INFO @ Sat, 11 Dec 2021 12:12:03: #2 number of paired peaks: 92 WARNING @ Sat, 11 Dec 2021 12:12:03: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:12:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:12:06: #1 tag size is determined as 101 bps INFO @ Sat, 11 Dec 2021 12:12:06: #1 tag size = 101 INFO @ Sat, 11 Dec 2021 12:12:06: #1 total tags in treatment: 12269181 INFO @ Sat, 11 Dec 2021 12:12:06: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:12:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:12:06: #1 tags after filtering in treatment: 11229819 INFO @ Sat, 11 Dec 2021 12:12:06: #1 Redundant rate of treatment: 0.08 INFO @ Sat, 11 Dec 2021 12:12:06: #1 finished! INFO @ Sat, 11 Dec 2021 12:12:06: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:12:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:12:07: #2 number of paired peaks: 92 WARNING @ Sat, 11 Dec 2021 12:12:07: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:12:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:12:09: 25000000 INFO @ Sat, 11 Dec 2021 12:12:16: 26000000 INFO @ Sat, 11 Dec 2021 12:12:22: 27000000 INFO @ Sat, 11 Dec 2021 12:12:28: 28000000 INFO @ Sat, 11 Dec 2021 12:12:31: #1 tag size is determined as 101 bps INFO @ Sat, 11 Dec 2021 12:12:31: #1 tag size = 101 INFO @ Sat, 11 Dec 2021 12:12:31: #1 total tags in treatment: 12269181 INFO @ Sat, 11 Dec 2021 12:12:31: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:12:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:12:31: #1 tags after filtering in treatment: 11229819 INFO @ Sat, 11 Dec 2021 12:12:31: #1 Redundant rate of treatment: 0.08 INFO @ Sat, 11 Dec 2021 12:12:31: #1 finished! INFO @ Sat, 11 Dec 2021 12:12:31: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:12:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:12:32: #2 number of paired peaks: 92 WARNING @ Sat, 11 Dec 2021 12:12:32: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:12:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9711626/SRX9711626.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling