Job ID = 6528524 SRX = SRX969930 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:18:03 prefetch.2.10.7: 1) Downloading 'SRR1931646'... 2020-06-29T15:18:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:20:04 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:20:04 prefetch.2.10.7: 1) 'SRR1931646' was downloaded successfully Read 28962246 spots for SRR1931646/SRR1931646.sra Written 28962246 spots for SRR1931646/SRR1931646.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:07 28962246 reads; of these: 28962246 (100.00%) were unpaired; of these: 883810 (3.05%) aligned 0 times 20533727 (70.90%) aligned exactly 1 time 7544709 (26.05%) aligned >1 times 96.95% overall alignment rate Time searching: 00:10:07 Overall time: 00:10:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3732178 / 28078436 = 0.1329 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:43:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:43:31: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:43:31: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:43:36: 1000000 INFO @ Tue, 30 Jun 2020 00:43:41: 2000000 INFO @ Tue, 30 Jun 2020 00:43:46: 3000000 INFO @ Tue, 30 Jun 2020 00:43:51: 4000000 INFO @ Tue, 30 Jun 2020 00:43:56: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:44:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:44:01: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:44:01: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:44:01: 6000000 INFO @ Tue, 30 Jun 2020 00:44:06: 1000000 INFO @ Tue, 30 Jun 2020 00:44:06: 7000000 INFO @ Tue, 30 Jun 2020 00:44:12: 2000000 INFO @ Tue, 30 Jun 2020 00:44:12: 8000000 INFO @ Tue, 30 Jun 2020 00:44:17: 3000000 INFO @ Tue, 30 Jun 2020 00:44:17: 9000000 INFO @ Tue, 30 Jun 2020 00:44:22: 4000000 INFO @ Tue, 30 Jun 2020 00:44:22: 10000000 INFO @ Tue, 30 Jun 2020 00:44:28: 5000000 INFO @ Tue, 30 Jun 2020 00:44:28: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:44:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:44:31: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:44:31: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:44:33: 6000000 INFO @ Tue, 30 Jun 2020 00:44:33: 12000000 INFO @ Tue, 30 Jun 2020 00:44:36: 1000000 INFO @ Tue, 30 Jun 2020 00:44:38: 7000000 INFO @ Tue, 30 Jun 2020 00:44:38: 13000000 INFO @ Tue, 30 Jun 2020 00:44:42: 2000000 INFO @ Tue, 30 Jun 2020 00:44:44: 8000000 INFO @ Tue, 30 Jun 2020 00:44:44: 14000000 INFO @ Tue, 30 Jun 2020 00:44:47: 3000000 INFO @ Tue, 30 Jun 2020 00:44:49: 9000000 INFO @ Tue, 30 Jun 2020 00:44:49: 15000000 INFO @ Tue, 30 Jun 2020 00:44:52: 4000000 INFO @ Tue, 30 Jun 2020 00:44:55: 10000000 INFO @ Tue, 30 Jun 2020 00:44:55: 16000000 INFO @ Tue, 30 Jun 2020 00:44:58: 5000000 INFO @ Tue, 30 Jun 2020 00:45:00: 11000000 INFO @ Tue, 30 Jun 2020 00:45:00: 17000000 INFO @ Tue, 30 Jun 2020 00:45:03: 6000000 INFO @ Tue, 30 Jun 2020 00:45:06: 12000000 INFO @ Tue, 30 Jun 2020 00:45:06: 18000000 INFO @ Tue, 30 Jun 2020 00:45:08: 7000000 INFO @ Tue, 30 Jun 2020 00:45:11: 13000000 INFO @ Tue, 30 Jun 2020 00:45:11: 19000000 INFO @ Tue, 30 Jun 2020 00:45:14: 8000000 INFO @ Tue, 30 Jun 2020 00:45:16: 14000000 INFO @ Tue, 30 Jun 2020 00:45:17: 20000000 INFO @ Tue, 30 Jun 2020 00:45:19: 9000000 INFO @ Tue, 30 Jun 2020 00:45:22: 15000000 INFO @ Tue, 30 Jun 2020 00:45:22: 21000000 INFO @ Tue, 30 Jun 2020 00:45:25: 10000000 INFO @ Tue, 30 Jun 2020 00:45:27: 16000000 INFO @ Tue, 30 Jun 2020 00:45:28: 22000000 INFO @ Tue, 30 Jun 2020 00:45:30: 11000000 INFO @ Tue, 30 Jun 2020 00:45:33: 17000000 INFO @ Tue, 30 Jun 2020 00:45:33: 23000000 INFO @ Tue, 30 Jun 2020 00:45:36: 12000000 INFO @ Tue, 30 Jun 2020 00:45:38: 18000000 INFO @ Tue, 30 Jun 2020 00:45:39: 24000000 INFO @ Tue, 30 Jun 2020 00:45:41: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:45:41: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:45:41: #1 total tags in treatment: 24346258 INFO @ Tue, 30 Jun 2020 00:45:41: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:45:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:45:41: 13000000 INFO @ Tue, 30 Jun 2020 00:45:41: #1 tags after filtering in treatment: 24346258 INFO @ Tue, 30 Jun 2020 00:45:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:45:41: #1 finished! INFO @ Tue, 30 Jun 2020 00:45:41: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:45:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:45:43: #2 number of paired peaks: 89 WARNING @ Tue, 30 Jun 2020 00:45:43: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:45:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:45:44: 19000000 INFO @ Tue, 30 Jun 2020 00:45:46: 14000000 INFO @ Tue, 30 Jun 2020 00:45:49: 20000000 INFO @ Tue, 30 Jun 2020 00:45:52: 15000000 INFO @ Tue, 30 Jun 2020 00:45:55: 21000000 INFO @ Tue, 30 Jun 2020 00:45:57: 16000000 INFO @ Tue, 30 Jun 2020 00:46:00: 22000000 INFO @ Tue, 30 Jun 2020 00:46:03: 17000000 INFO @ Tue, 30 Jun 2020 00:46:05: 23000000 INFO @ Tue, 30 Jun 2020 00:46:08: 18000000 INFO @ Tue, 30 Jun 2020 00:46:11: 24000000 INFO @ Tue, 30 Jun 2020 00:46:13: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:46:13: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:46:13: #1 total tags in treatment: 24346258 INFO @ Tue, 30 Jun 2020 00:46:13: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:46:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:46:13: #1 tags after filtering in treatment: 24346258 INFO @ Tue, 30 Jun 2020 00:46:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:46:13: #1 finished! INFO @ Tue, 30 Jun 2020 00:46:13: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:46:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:46:13: 19000000 INFO @ Tue, 30 Jun 2020 00:46:15: #2 number of paired peaks: 89 WARNING @ Tue, 30 Jun 2020 00:46:15: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:46:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:46:19: 20000000 INFO @ Tue, 30 Jun 2020 00:46:24: 21000000 INFO @ Tue, 30 Jun 2020 00:46:29: 22000000 INFO @ Tue, 30 Jun 2020 00:46:34: 23000000 INFO @ Tue, 30 Jun 2020 00:46:40: 24000000 INFO @ Tue, 30 Jun 2020 00:46:42: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:46:42: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:46:42: #1 total tags in treatment: 24346258 INFO @ Tue, 30 Jun 2020 00:46:42: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:46:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:46:42: #1 tags after filtering in treatment: 24346258 INFO @ Tue, 30 Jun 2020 00:46:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:46:42: #1 finished! INFO @ Tue, 30 Jun 2020 00:46:42: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:46:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:46:43: #2 number of paired peaks: 89 WARNING @ Tue, 30 Jun 2020 00:46:43: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:46:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969930/SRX969930.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。