Job ID = 9159949 sra ファイルのダウンロード中... Completed: 1169851K bytes transferred in 14 seconds (662565K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 35931394 spots for /home/okishinya/chipatlas/results/dm3/SRX969924/SRR1931640.sra Written 35931394 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:00 35931394 reads; of these: 35931394 (100.00%) were unpaired; of these: 1059998 (2.95%) aligned 0 times 24337780 (67.73%) aligned exactly 1 time 10533616 (29.32%) aligned >1 times 97.05% overall alignment rate Time searching: 00:16:00 Overall time: 00:16:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6770799 / 34871396 = 0.1942 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 01:44:31: # Command line: callpeak -t SRX969924.bam -f BAM -g dm -n SRX969924.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX969924.20 # format = BAM # ChIP-seq file = ['SRX969924.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:44:31: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:44:31: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:44:31: # Command line: callpeak -t SRX969924.bam -f BAM -g dm -n SRX969924.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX969924.05 # format = BAM # ChIP-seq file = ['SRX969924.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:44:31: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:44:31: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:44:31: # Command line: callpeak -t SRX969924.bam -f BAM -g dm -n SRX969924.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX969924.10 # format = BAM # ChIP-seq file = ['SRX969924.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:44:31: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:44:31: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:44:39: 1000000 INFO @ Wed, 28 Jun 2017 01:44:39: 1000000 INFO @ Wed, 28 Jun 2017 01:44:39: 1000000 INFO @ Wed, 28 Jun 2017 01:44:47: 2000000 INFO @ Wed, 28 Jun 2017 01:44:47: 2000000 INFO @ Wed, 28 Jun 2017 01:44:47: 2000000 INFO @ Wed, 28 Jun 2017 01:44:55: 3000000 INFO @ Wed, 28 Jun 2017 01:44:55: 3000000 INFO @ Wed, 28 Jun 2017 01:44:55: 3000000 INFO @ Wed, 28 Jun 2017 01:45:03: 4000000 INFO @ Wed, 28 Jun 2017 01:45:03: 4000000 INFO @ Wed, 28 Jun 2017 01:45:03: 4000000 INFO @ Wed, 28 Jun 2017 01:45:11: 5000000 INFO @ Wed, 28 Jun 2017 01:45:11: 5000000 INFO @ Wed, 28 Jun 2017 01:45:11: 5000000 INFO @ Wed, 28 Jun 2017 01:45:19: 6000000 INFO @ Wed, 28 Jun 2017 01:45:19: 6000000 INFO @ Wed, 28 Jun 2017 01:45:19: 6000000 INFO @ Wed, 28 Jun 2017 01:45:27: 7000000 INFO @ Wed, 28 Jun 2017 01:45:27: 7000000 INFO @ Wed, 28 Jun 2017 01:45:27: 7000000 INFO @ Wed, 28 Jun 2017 01:45:35: 8000000 INFO @ Wed, 28 Jun 2017 01:45:35: 8000000 INFO @ Wed, 28 Jun 2017 01:45:35: 8000000 INFO @ Wed, 28 Jun 2017 01:45:43: 9000000 INFO @ Wed, 28 Jun 2017 01:45:43: 9000000 INFO @ Wed, 28 Jun 2017 01:45:43: 9000000 INFO @ Wed, 28 Jun 2017 01:45:51: 10000000 INFO @ Wed, 28 Jun 2017 01:45:51: 10000000 INFO @ Wed, 28 Jun 2017 01:45:51: 10000000 INFO @ Wed, 28 Jun 2017 01:45:58: 11000000 INFO @ Wed, 28 Jun 2017 01:45:59: 11000000 INFO @ Wed, 28 Jun 2017 01:45:59: 11000000 INFO @ Wed, 28 Jun 2017 01:46:05: 12000000 INFO @ Wed, 28 Jun 2017 01:46:07: 12000000 INFO @ Wed, 28 Jun 2017 01:46:07: 12000000 INFO @ Wed, 28 Jun 2017 01:46:13: 13000000 INFO @ Wed, 28 Jun 2017 01:46:14: 13000000 INFO @ Wed, 28 Jun 2017 01:46:14: 13000000 INFO @ Wed, 28 Jun 2017 01:46:20: 14000000 INFO @ Wed, 28 Jun 2017 01:46:22: 14000000 INFO @ Wed, 28 Jun 2017 01:46:22: 14000000 INFO @ Wed, 28 Jun 2017 01:46:27: 15000000 INFO @ Wed, 28 Jun 2017 01:46:30: 15000000 INFO @ Wed, 28 Jun 2017 01:46:30: 15000000 INFO @ Wed, 28 Jun 2017 01:46:35: 16000000 INFO @ Wed, 28 Jun 2017 01:46:38: 16000000 INFO @ Wed, 28 Jun 2017 01:46:38: 16000000 INFO @ Wed, 28 Jun 2017 01:46:42: 17000000 INFO @ Wed, 28 Jun 2017 01:46:46: 17000000 INFO @ Wed, 28 Jun 2017 01:46:46: 17000000 INFO @ Wed, 28 Jun 2017 01:46:49: 18000000 INFO @ Wed, 28 Jun 2017 01:46:54: 18000000 INFO @ Wed, 28 Jun 2017 01:46:54: 18000000 INFO @ Wed, 28 Jun 2017 01:46:57: 19000000 INFO @ Wed, 28 Jun 2017 01:47:02: 19000000 INFO @ Wed, 28 Jun 2017 01:47:02: 19000000 INFO @ Wed, 28 Jun 2017 01:47:04: 20000000 INFO @ Wed, 28 Jun 2017 01:47:09: 20000000 INFO @ Wed, 28 Jun 2017 01:47:10: 20000000 INFO @ Wed, 28 Jun 2017 01:47:12: 21000000 INFO @ Wed, 28 Jun 2017 01:47:18: 21000000 INFO @ Wed, 28 Jun 2017 01:47:18: 21000000 INFO @ Wed, 28 Jun 2017 01:47:19: 22000000 INFO @ Wed, 28 Jun 2017 01:47:26: 22000000 INFO @ Wed, 28 Jun 2017 01:47:26: 22000000 INFO @ Wed, 28 Jun 2017 01:47:27: 23000000 INFO @ Wed, 28 Jun 2017 01:47:34: 24000000 INFO @ Wed, 28 Jun 2017 01:47:34: 23000000 INFO @ Wed, 28 Jun 2017 01:47:34: 23000000 INFO @ Wed, 28 Jun 2017 01:47:42: 25000000 INFO @ Wed, 28 Jun 2017 01:47:43: 24000000 INFO @ Wed, 28 Jun 2017 01:47:43: 24000000 INFO @ Wed, 28 Jun 2017 01:47:49: 26000000 INFO @ Wed, 28 Jun 2017 01:47:51: 25000000 INFO @ Wed, 28 Jun 2017 01:47:51: 25000000 INFO @ Wed, 28 Jun 2017 01:47:56: 27000000 INFO @ Wed, 28 Jun 2017 01:47:59: 26000000 INFO @ Wed, 28 Jun 2017 01:47:59: 26000000 INFO @ Wed, 28 Jun 2017 01:48:04: 28000000 INFO @ Wed, 28 Jun 2017 01:48:04: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 01:48:04: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 01:48:04: #1 total tags in treatment: 28100597 INFO @ Wed, 28 Jun 2017 01:48:04: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:48:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:48:05: #1 tags after filtering in treatment: 28100597 INFO @ Wed, 28 Jun 2017 01:48:05: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:48:05: #1 finished! INFO @ Wed, 28 Jun 2017 01:48:05: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:48:05: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:48:07: 27000000 INFO @ Wed, 28 Jun 2017 01:48:07: #2 number of paired peaks: 66 WARNING @ Wed, 28 Jun 2017 01:48:07: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:48:07: Process for pairing-model is terminated! cat: SRX969924.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Wed, 28 Jun 2017 01:48:07: 27000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX969924.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX969924.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX969924.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 01:48:15: 28000000 INFO @ Wed, 28 Jun 2017 01:48:15: 28000000 INFO @ Wed, 28 Jun 2017 01:48:16: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 01:48:16: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 01:48:16: #1 total tags in treatment: 28100597 INFO @ Wed, 28 Jun 2017 01:48:16: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:48:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:48:16: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 01:48:16: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 01:48:16: #1 total tags in treatment: 28100597 INFO @ Wed, 28 Jun 2017 01:48:16: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:48:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:48:16: #1 tags after filtering in treatment: 28100597 INFO @ Wed, 28 Jun 2017 01:48:16: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:48:16: #1 finished! INFO @ Wed, 28 Jun 2017 01:48:16: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:48:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:48:16: #1 tags after filtering in treatment: 28100597 INFO @ Wed, 28 Jun 2017 01:48:16: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:48:16: #1 finished! INFO @ Wed, 28 Jun 2017 01:48:16: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:48:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:48:18: #2 number of paired peaks: 66 WARNING @ Wed, 28 Jun 2017 01:48:18: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:48:18: Process for pairing-model is terminated! cat: SRX969924.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX969924.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX969924.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX969924.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 01:48:18: #2 number of paired peaks: 66 WARNING @ Wed, 28 Jun 2017 01:48:18: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:48:18: Process for pairing-model is terminated! cat: SRX969924.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX969924.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX969924.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX969924.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。