Job ID = 6528520 SRX = SRX969915 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:59:58 prefetch.2.10.7: 1) Downloading 'SRR1931631'... 2020-06-29T14:59:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:02:41 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:02:41 prefetch.2.10.7: 1) 'SRR1931631' was downloaded successfully Read 26029709 spots for SRR1931631/SRR1931631.sra Written 26029709 spots for SRR1931631/SRR1931631.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:54 26029709 reads; of these: 26029709 (100.00%) were unpaired; of these: 675283 (2.59%) aligned 0 times 18809369 (72.26%) aligned exactly 1 time 6545057 (25.14%) aligned >1 times 97.41% overall alignment rate Time searching: 00:08:54 Overall time: 00:08:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3159257 / 25354426 = 0.1246 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:24:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:24:22: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:24:22: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:24:28: 1000000 INFO @ Tue, 30 Jun 2020 00:24:34: 2000000 INFO @ Tue, 30 Jun 2020 00:24:39: 3000000 INFO @ Tue, 30 Jun 2020 00:24:45: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:24:50: 5000000 INFO @ Tue, 30 Jun 2020 00:24:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:24:52: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:24:52: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:24:56: 6000000 INFO @ Tue, 30 Jun 2020 00:24:58: 1000000 INFO @ Tue, 30 Jun 2020 00:25:02: 7000000 INFO @ Tue, 30 Jun 2020 00:25:04: 2000000 INFO @ Tue, 30 Jun 2020 00:25:08: 8000000 INFO @ Tue, 30 Jun 2020 00:25:10: 3000000 INFO @ Tue, 30 Jun 2020 00:25:14: 9000000 INFO @ Tue, 30 Jun 2020 00:25:16: 4000000 INFO @ Tue, 30 Jun 2020 00:25:20: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:25:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:25:22: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:25:22: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:25:22: 5000000 INFO @ Tue, 30 Jun 2020 00:25:26: 11000000 INFO @ Tue, 30 Jun 2020 00:25:28: 1000000 INFO @ Tue, 30 Jun 2020 00:25:28: 6000000 INFO @ Tue, 30 Jun 2020 00:25:32: 12000000 INFO @ Tue, 30 Jun 2020 00:25:34: 2000000 INFO @ Tue, 30 Jun 2020 00:25:34: 7000000 INFO @ Tue, 30 Jun 2020 00:25:37: 13000000 INFO @ Tue, 30 Jun 2020 00:25:40: 3000000 INFO @ Tue, 30 Jun 2020 00:25:41: 8000000 INFO @ Tue, 30 Jun 2020 00:25:43: 14000000 INFO @ Tue, 30 Jun 2020 00:25:46: 4000000 INFO @ Tue, 30 Jun 2020 00:25:47: 9000000 INFO @ Tue, 30 Jun 2020 00:25:49: 15000000 INFO @ Tue, 30 Jun 2020 00:25:52: 5000000 INFO @ Tue, 30 Jun 2020 00:25:53: 10000000 INFO @ Tue, 30 Jun 2020 00:25:55: 16000000 INFO @ Tue, 30 Jun 2020 00:25:58: 6000000 INFO @ Tue, 30 Jun 2020 00:25:59: 11000000 INFO @ Tue, 30 Jun 2020 00:26:01: 17000000 INFO @ Tue, 30 Jun 2020 00:26:04: 7000000 INFO @ Tue, 30 Jun 2020 00:26:05: 12000000 INFO @ Tue, 30 Jun 2020 00:26:07: 18000000 INFO @ Tue, 30 Jun 2020 00:26:11: 8000000 INFO @ Tue, 30 Jun 2020 00:26:11: 13000000 INFO @ Tue, 30 Jun 2020 00:26:13: 19000000 INFO @ Tue, 30 Jun 2020 00:26:17: 9000000 INFO @ Tue, 30 Jun 2020 00:26:17: 14000000 INFO @ Tue, 30 Jun 2020 00:26:19: 20000000 INFO @ Tue, 30 Jun 2020 00:26:23: 10000000 INFO @ Tue, 30 Jun 2020 00:26:23: 15000000 INFO @ Tue, 30 Jun 2020 00:26:24: 21000000 INFO @ Tue, 30 Jun 2020 00:26:28: 11000000 INFO @ Tue, 30 Jun 2020 00:26:29: 16000000 INFO @ Tue, 30 Jun 2020 00:26:30: 22000000 INFO @ Tue, 30 Jun 2020 00:26:32: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:26:32: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:26:32: #1 total tags in treatment: 22195169 INFO @ Tue, 30 Jun 2020 00:26:32: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:26:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:26:32: #1 tags after filtering in treatment: 22195169 INFO @ Tue, 30 Jun 2020 00:26:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:26:32: #1 finished! INFO @ Tue, 30 Jun 2020 00:26:32: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:26:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:26:33: #2 number of paired peaks: 34 WARNING @ Tue, 30 Jun 2020 00:26:33: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:26:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:26:34: 12000000 INFO @ Tue, 30 Jun 2020 00:26:35: 17000000 INFO @ Tue, 30 Jun 2020 00:26:40: 13000000 INFO @ Tue, 30 Jun 2020 00:26:40: 18000000 INFO @ Tue, 30 Jun 2020 00:26:46: 14000000 INFO @ Tue, 30 Jun 2020 00:26:47: 19000000 INFO @ Tue, 30 Jun 2020 00:26:52: 15000000 INFO @ Tue, 30 Jun 2020 00:26:53: 20000000 INFO @ Tue, 30 Jun 2020 00:26:58: 16000000 INFO @ Tue, 30 Jun 2020 00:26:58: 21000000 INFO @ Tue, 30 Jun 2020 00:27:04: 17000000 INFO @ Tue, 30 Jun 2020 00:27:05: 22000000 INFO @ Tue, 30 Jun 2020 00:27:06: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:27:06: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:27:06: #1 total tags in treatment: 22195169 INFO @ Tue, 30 Jun 2020 00:27:06: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:27:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:27:06: #1 tags after filtering in treatment: 22195169 INFO @ Tue, 30 Jun 2020 00:27:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:27:06: #1 finished! INFO @ Tue, 30 Jun 2020 00:27:06: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:27:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:27:08: #2 number of paired peaks: 34 WARNING @ Tue, 30 Jun 2020 00:27:08: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:27:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:27:10: 18000000 INFO @ Tue, 30 Jun 2020 00:27:15: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:27:21: 20000000 INFO @ Tue, 30 Jun 2020 00:27:27: 21000000 INFO @ Tue, 30 Jun 2020 00:27:33: 22000000 INFO @ Tue, 30 Jun 2020 00:27:34: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:27:34: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:27:34: #1 total tags in treatment: 22195169 INFO @ Tue, 30 Jun 2020 00:27:34: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:27:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:27:35: #1 tags after filtering in treatment: 22195169 INFO @ Tue, 30 Jun 2020 00:27:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:27:35: #1 finished! INFO @ Tue, 30 Jun 2020 00:27:35: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:27:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:27:36: #2 number of paired peaks: 34 WARNING @ Tue, 30 Jun 2020 00:27:36: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:27:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969915/SRX969915.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。