Job ID = 6528517 SRX = SRX969910 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:33:42 prefetch.2.10.7: 1) Downloading 'SRR1931626'... 2020-06-29T15:33:42 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:38:47 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:38:47 prefetch.2.10.7: 1) 'SRR1931626' was downloaded successfully Read 43236646 spots for SRR1931626/SRR1931626.sra Written 43236646 spots for SRR1931626/SRR1931626.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:28 43236646 reads; of these: 43236646 (100.00%) were unpaired; of these: 6131253 (14.18%) aligned 0 times 29126439 (67.37%) aligned exactly 1 time 7978954 (18.45%) aligned >1 times 85.82% overall alignment rate Time searching: 00:12:28 Overall time: 00:12:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5718659 / 37105393 = 0.1541 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:12:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:12:32: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:12:32: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:12:36: 1000000 INFO @ Tue, 30 Jun 2020 01:12:41: 2000000 INFO @ Tue, 30 Jun 2020 01:12:46: 3000000 INFO @ Tue, 30 Jun 2020 01:12:50: 4000000 INFO @ Tue, 30 Jun 2020 01:12:55: 5000000 INFO @ Tue, 30 Jun 2020 01:13:00: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:13:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:13:02: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:13:02: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:13:04: 7000000 INFO @ Tue, 30 Jun 2020 01:13:07: 1000000 INFO @ Tue, 30 Jun 2020 01:13:09: 8000000 INFO @ Tue, 30 Jun 2020 01:13:12: 2000000 INFO @ Tue, 30 Jun 2020 01:13:14: 9000000 INFO @ Tue, 30 Jun 2020 01:13:16: 3000000 INFO @ Tue, 30 Jun 2020 01:13:18: 10000000 INFO @ Tue, 30 Jun 2020 01:13:21: 4000000 INFO @ Tue, 30 Jun 2020 01:13:23: 11000000 INFO @ Tue, 30 Jun 2020 01:13:26: 5000000 INFO @ Tue, 30 Jun 2020 01:13:28: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:13:30: 6000000 INFO @ Tue, 30 Jun 2020 01:13:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:13:32: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:13:32: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:13:32: 13000000 INFO @ Tue, 30 Jun 2020 01:13:35: 7000000 INFO @ Tue, 30 Jun 2020 01:13:36: 1000000 INFO @ Tue, 30 Jun 2020 01:13:37: 14000000 INFO @ Tue, 30 Jun 2020 01:13:40: 8000000 INFO @ Tue, 30 Jun 2020 01:13:41: 2000000 INFO @ Tue, 30 Jun 2020 01:13:42: 15000000 INFO @ Tue, 30 Jun 2020 01:13:45: 9000000 INFO @ Tue, 30 Jun 2020 01:13:46: 3000000 INFO @ Tue, 30 Jun 2020 01:13:46: 16000000 INFO @ Tue, 30 Jun 2020 01:13:50: 10000000 INFO @ Tue, 30 Jun 2020 01:13:51: 4000000 INFO @ Tue, 30 Jun 2020 01:13:51: 17000000 INFO @ Tue, 30 Jun 2020 01:13:55: 11000000 INFO @ Tue, 30 Jun 2020 01:13:55: 5000000 INFO @ Tue, 30 Jun 2020 01:13:55: 18000000 INFO @ Tue, 30 Jun 2020 01:13:59: 12000000 INFO @ Tue, 30 Jun 2020 01:14:00: 6000000 INFO @ Tue, 30 Jun 2020 01:14:00: 19000000 INFO @ Tue, 30 Jun 2020 01:14:04: 13000000 INFO @ Tue, 30 Jun 2020 01:14:05: 7000000 INFO @ Tue, 30 Jun 2020 01:14:05: 20000000 INFO @ Tue, 30 Jun 2020 01:14:09: 14000000 INFO @ Tue, 30 Jun 2020 01:14:09: 8000000 INFO @ Tue, 30 Jun 2020 01:14:09: 21000000 INFO @ Tue, 30 Jun 2020 01:14:13: 15000000 INFO @ Tue, 30 Jun 2020 01:14:14: 9000000 INFO @ Tue, 30 Jun 2020 01:14:14: 22000000 INFO @ Tue, 30 Jun 2020 01:14:18: 16000000 INFO @ Tue, 30 Jun 2020 01:14:19: 10000000 INFO @ Tue, 30 Jun 2020 01:14:19: 23000000 INFO @ Tue, 30 Jun 2020 01:14:23: 17000000 INFO @ Tue, 30 Jun 2020 01:14:23: 11000000 INFO @ Tue, 30 Jun 2020 01:14:23: 24000000 INFO @ Tue, 30 Jun 2020 01:14:27: 18000000 INFO @ Tue, 30 Jun 2020 01:14:28: 12000000 INFO @ Tue, 30 Jun 2020 01:14:28: 25000000 INFO @ Tue, 30 Jun 2020 01:14:32: 19000000 INFO @ Tue, 30 Jun 2020 01:14:33: 13000000 INFO @ Tue, 30 Jun 2020 01:14:33: 26000000 INFO @ Tue, 30 Jun 2020 01:14:36: 20000000 INFO @ Tue, 30 Jun 2020 01:14:37: 14000000 INFO @ Tue, 30 Jun 2020 01:14:37: 27000000 INFO @ Tue, 30 Jun 2020 01:14:41: 21000000 INFO @ Tue, 30 Jun 2020 01:14:42: 15000000 INFO @ Tue, 30 Jun 2020 01:14:42: 28000000 INFO @ Tue, 30 Jun 2020 01:14:46: 22000000 INFO @ Tue, 30 Jun 2020 01:14:47: 16000000 INFO @ Tue, 30 Jun 2020 01:14:47: 29000000 INFO @ Tue, 30 Jun 2020 01:14:50: 23000000 INFO @ Tue, 30 Jun 2020 01:14:51: 17000000 INFO @ Tue, 30 Jun 2020 01:14:51: 30000000 INFO @ Tue, 30 Jun 2020 01:14:55: 24000000 INFO @ Tue, 30 Jun 2020 01:14:56: 18000000 INFO @ Tue, 30 Jun 2020 01:14:56: 31000000 INFO @ Tue, 30 Jun 2020 01:14:58: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 01:14:58: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 01:14:58: #1 total tags in treatment: 31386734 INFO @ Tue, 30 Jun 2020 01:14:58: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:14:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:14:59: #1 tags after filtering in treatment: 31386734 INFO @ Tue, 30 Jun 2020 01:14:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:14:59: #1 finished! INFO @ Tue, 30 Jun 2020 01:14:59: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:14:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:15:00: 25000000 INFO @ Tue, 30 Jun 2020 01:15:00: 19000000 INFO @ Tue, 30 Jun 2020 01:15:00: #2 number of paired peaks: 18 WARNING @ Tue, 30 Jun 2020 01:15:00: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:15:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:15:05: 26000000 INFO @ Tue, 30 Jun 2020 01:15:05: 20000000 INFO @ Tue, 30 Jun 2020 01:15:09: 27000000 INFO @ Tue, 30 Jun 2020 01:15:10: 21000000 INFO @ Tue, 30 Jun 2020 01:15:14: 28000000 INFO @ Tue, 30 Jun 2020 01:15:14: 22000000 INFO @ Tue, 30 Jun 2020 01:15:18: 29000000 INFO @ Tue, 30 Jun 2020 01:15:19: 23000000 INFO @ Tue, 30 Jun 2020 01:15:23: 30000000 INFO @ Tue, 30 Jun 2020 01:15:24: 24000000 INFO @ Tue, 30 Jun 2020 01:15:28: 31000000 INFO @ Tue, 30 Jun 2020 01:15:28: 25000000 INFO @ Tue, 30 Jun 2020 01:15:30: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 01:15:30: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 01:15:30: #1 total tags in treatment: 31386734 INFO @ Tue, 30 Jun 2020 01:15:30: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:15:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:15:30: #1 tags after filtering in treatment: 31386734 INFO @ Tue, 30 Jun 2020 01:15:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:15:30: #1 finished! INFO @ Tue, 30 Jun 2020 01:15:30: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:15:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:15:32: #2 number of paired peaks: 18 WARNING @ Tue, 30 Jun 2020 01:15:32: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:15:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:15:33: 26000000 INFO @ Tue, 30 Jun 2020 01:15:38: 27000000 INFO @ Tue, 30 Jun 2020 01:15:42: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:15:47: 29000000 INFO @ Tue, 30 Jun 2020 01:15:51: 30000000 INFO @ Tue, 30 Jun 2020 01:15:56: 31000000 INFO @ Tue, 30 Jun 2020 01:15:58: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 01:15:58: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 01:15:58: #1 total tags in treatment: 31386734 INFO @ Tue, 30 Jun 2020 01:15:58: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:15:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:15:58: #1 tags after filtering in treatment: 31386734 INFO @ Tue, 30 Jun 2020 01:15:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:15:58: #1 finished! INFO @ Tue, 30 Jun 2020 01:15:58: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:15:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:16:01: #2 number of paired peaks: 18 WARNING @ Tue, 30 Jun 2020 01:16:01: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:16:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969910/SRX969910.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。