Job ID = 16439461 SRX = SRX9518319 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 20209290 spots for SRR13070802/SRR13070802.sra Written 20209290 spots for SRR13070802/SRR13070802.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439879 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:06:44 20209290 reads; of these: 20209290 (100.00%) were paired; of these: 1219310 (6.03%) aligned concordantly 0 times 13639372 (67.49%) aligned concordantly exactly 1 time 5350608 (26.48%) aligned concordantly >1 times ---- 1219310 pairs aligned concordantly 0 times; of these: 129275 (10.60%) aligned discordantly 1 time ---- 1090035 pairs aligned 0 times concordantly or discordantly; of these: 2180070 mates make up the pairs; of these: 1371991 (62.93%) aligned 0 times 403562 (18.51%) aligned exactly 1 time 404517 (18.56%) aligned >1 times 96.61% overall alignment rate Time searching: 01:06:44 Overall time: 01:06:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 528606 / 19081120 = 0.0277 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:43:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:43:05: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:43:05: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:43:14: 1000000 INFO @ Tue, 02 Aug 2022 16:43:23: 2000000 INFO @ Tue, 02 Aug 2022 16:43:32: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:43:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:43:35: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:43:35: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:43:41: 4000000 INFO @ Tue, 02 Aug 2022 16:43:44: 1000000 INFO @ Tue, 02 Aug 2022 16:43:49: 5000000 INFO @ Tue, 02 Aug 2022 16:43:53: 2000000 INFO @ Tue, 02 Aug 2022 16:43:58: 6000000 INFO @ Tue, 02 Aug 2022 16:44:01: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:44:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:44:05: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:44:05: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:44:06: 7000000 INFO @ Tue, 02 Aug 2022 16:44:11: 4000000 INFO @ Tue, 02 Aug 2022 16:44:14: 1000000 INFO @ Tue, 02 Aug 2022 16:44:15: 8000000 INFO @ Tue, 02 Aug 2022 16:44:21: 5000000 INFO @ Tue, 02 Aug 2022 16:44:24: 2000000 INFO @ Tue, 02 Aug 2022 16:44:24: 9000000 INFO @ Tue, 02 Aug 2022 16:44:30: 6000000 INFO @ Tue, 02 Aug 2022 16:44:33: 3000000 INFO @ Tue, 02 Aug 2022 16:44:34: 10000000 INFO @ Tue, 02 Aug 2022 16:44:41: 7000000 INFO @ Tue, 02 Aug 2022 16:44:43: 4000000 INFO @ Tue, 02 Aug 2022 16:44:44: 11000000 INFO @ Tue, 02 Aug 2022 16:44:51: 8000000 INFO @ Tue, 02 Aug 2022 16:44:52: 5000000 INFO @ Tue, 02 Aug 2022 16:44:54: 12000000 INFO @ Tue, 02 Aug 2022 16:45:01: 9000000 INFO @ Tue, 02 Aug 2022 16:45:01: 6000000 INFO @ Tue, 02 Aug 2022 16:45:05: 13000000 INFO @ Tue, 02 Aug 2022 16:45:11: 7000000 INFO @ Tue, 02 Aug 2022 16:45:11: 10000000 INFO @ Tue, 02 Aug 2022 16:45:15: 14000000 INFO @ Tue, 02 Aug 2022 16:45:21: 8000000 INFO @ Tue, 02 Aug 2022 16:45:21: 11000000 INFO @ Tue, 02 Aug 2022 16:45:25: 15000000 INFO @ Tue, 02 Aug 2022 16:45:31: 9000000 INFO @ Tue, 02 Aug 2022 16:45:31: 12000000 INFO @ Tue, 02 Aug 2022 16:45:35: 16000000 INFO @ Tue, 02 Aug 2022 16:45:41: 10000000 INFO @ Tue, 02 Aug 2022 16:45:41: 13000000 INFO @ Tue, 02 Aug 2022 16:45:46: 17000000 INFO @ Tue, 02 Aug 2022 16:45:51: 14000000 INFO @ Tue, 02 Aug 2022 16:45:52: 11000000 INFO @ Tue, 02 Aug 2022 16:45:57: 18000000 INFO @ Tue, 02 Aug 2022 16:46:02: 15000000 INFO @ Tue, 02 Aug 2022 16:46:02: 12000000 INFO @ Tue, 02 Aug 2022 16:46:08: 19000000 INFO @ Tue, 02 Aug 2022 16:46:12: 16000000 INFO @ Tue, 02 Aug 2022 16:46:13: 13000000 INFO @ Tue, 02 Aug 2022 16:46:19: 20000000 INFO @ Tue, 02 Aug 2022 16:46:22: 17000000 INFO @ Tue, 02 Aug 2022 16:46:23: 14000000 INFO @ Tue, 02 Aug 2022 16:46:29: 21000000 INFO @ Tue, 02 Aug 2022 16:46:32: 18000000 INFO @ Tue, 02 Aug 2022 16:46:33: 15000000 INFO @ Tue, 02 Aug 2022 16:46:40: 22000000 INFO @ Tue, 02 Aug 2022 16:46:42: 16000000 INFO @ Tue, 02 Aug 2022 16:46:43: 19000000 INFO @ Tue, 02 Aug 2022 16:46:49: 23000000 INFO @ Tue, 02 Aug 2022 16:46:52: 17000000 INFO @ Tue, 02 Aug 2022 16:46:53: 20000000 INFO @ Tue, 02 Aug 2022 16:46:59: 24000000 INFO @ Tue, 02 Aug 2022 16:47:02: 18000000 INFO @ Tue, 02 Aug 2022 16:47:03: 21000000 INFO @ Tue, 02 Aug 2022 16:47:12: 25000000 INFO @ Tue, 02 Aug 2022 16:47:13: 19000000 INFO @ Tue, 02 Aug 2022 16:47:16: 22000000 INFO @ Tue, 02 Aug 2022 16:47:23: 26000000 INFO @ Tue, 02 Aug 2022 16:47:24: 20000000 INFO @ Tue, 02 Aug 2022 16:47:26: 23000000 INFO @ Tue, 02 Aug 2022 16:47:33: 27000000 INFO @ Tue, 02 Aug 2022 16:47:34: 21000000 INFO @ Tue, 02 Aug 2022 16:47:37: 24000000 INFO @ Tue, 02 Aug 2022 16:47:43: 28000000 INFO @ Tue, 02 Aug 2022 16:47:45: 22000000 INFO @ Tue, 02 Aug 2022 16:47:47: 25000000 INFO @ Tue, 02 Aug 2022 16:47:54: 29000000 INFO @ Tue, 02 Aug 2022 16:47:54: 23000000 INFO @ Tue, 02 Aug 2022 16:47:59: 26000000 INFO @ Tue, 02 Aug 2022 16:48:04: 24000000 INFO @ Tue, 02 Aug 2022 16:48:05: 30000000 INFO @ Tue, 02 Aug 2022 16:48:09: 27000000 INFO @ Tue, 02 Aug 2022 16:48:15: 25000000 INFO @ Tue, 02 Aug 2022 16:48:15: 31000000 INFO @ Tue, 02 Aug 2022 16:48:20: 28000000 INFO @ Tue, 02 Aug 2022 16:48:26: 26000000 INFO @ Tue, 02 Aug 2022 16:48:26: 32000000 INFO @ Tue, 02 Aug 2022 16:48:31: 29000000 INFO @ Tue, 02 Aug 2022 16:48:36: 27000000 INFO @ Tue, 02 Aug 2022 16:48:37: 33000000 INFO @ Tue, 02 Aug 2022 16:48:42: 30000000 INFO @ Tue, 02 Aug 2022 16:48:46: 28000000 INFO @ Tue, 02 Aug 2022 16:48:48: 34000000 INFO @ Tue, 02 Aug 2022 16:48:52: 31000000 INFO @ Tue, 02 Aug 2022 16:48:56: 29000000 INFO @ Tue, 02 Aug 2022 16:48:58: 35000000 INFO @ Tue, 02 Aug 2022 16:49:04: 32000000 INFO @ Tue, 02 Aug 2022 16:49:07: 30000000 INFO @ Tue, 02 Aug 2022 16:49:08: 36000000 INFO @ Tue, 02 Aug 2022 16:49:14: 33000000 INFO @ Tue, 02 Aug 2022 16:49:18: 31000000 INFO @ Tue, 02 Aug 2022 16:49:19: 37000000 INFO @ Tue, 02 Aug 2022 16:49:26: 34000000 INFO @ Tue, 02 Aug 2022 16:49:28: 32000000 INFO @ Tue, 02 Aug 2022 16:49:29: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:49:29: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:49:29: #1 total tags in treatment: 18461919 INFO @ Tue, 02 Aug 2022 16:49:29: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:49:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:49:30: #1 tags after filtering in treatment: 17261204 INFO @ Tue, 02 Aug 2022 16:49:30: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 16:49:30: #1 finished! INFO @ Tue, 02 Aug 2022 16:49:30: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:49:30: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:49:31: #2 number of paired peaks: 72 WARNING @ Tue, 02 Aug 2022 16:49:31: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:49:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:49:36: 35000000 INFO @ Tue, 02 Aug 2022 16:49:37: 33000000 INFO @ Tue, 02 Aug 2022 16:49:47: 34000000 INFO @ Tue, 02 Aug 2022 16:49:47: 36000000 INFO @ Tue, 02 Aug 2022 16:49:56: 35000000 INFO @ Tue, 02 Aug 2022 16:49:58: 37000000 INFO @ Tue, 02 Aug 2022 16:50:06: 36000000 INFO @ Tue, 02 Aug 2022 16:50:09: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:50:09: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:50:09: #1 total tags in treatment: 18461919 INFO @ Tue, 02 Aug 2022 16:50:09: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:50:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:50:10: #1 tags after filtering in treatment: 17261204 INFO @ Tue, 02 Aug 2022 16:50:10: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 16:50:10: #1 finished! INFO @ Tue, 02 Aug 2022 16:50:10: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:50:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:50:11: #2 number of paired peaks: 72 WARNING @ Tue, 02 Aug 2022 16:50:11: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:50:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:50:16: 37000000 INFO @ Tue, 02 Aug 2022 16:50:26: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:50:26: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:50:26: #1 total tags in treatment: 18461919 INFO @ Tue, 02 Aug 2022 16:50:26: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:50:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:50:27: #1 tags after filtering in treatment: 17261204 INFO @ Tue, 02 Aug 2022 16:50:27: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 16:50:27: #1 finished! INFO @ Tue, 02 Aug 2022 16:50:27: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:50:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:50:28: #2 number of paired peaks: 72 WARNING @ Tue, 02 Aug 2022 16:50:28: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:50:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518319/SRX9518319.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。