Job ID = 16439459 SRX = SRX9518317 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16323386 spots for SRR13070800/SRR13070800.sra Written 16323386 spots for SRR13070800/SRR13070800.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439662 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:06 16323386 reads; of these: 16323386 (100.00%) were paired; of these: 976913 (5.98%) aligned concordantly 0 times 11019554 (67.51%) aligned concordantly exactly 1 time 4326919 (26.51%) aligned concordantly >1 times ---- 976913 pairs aligned concordantly 0 times; of these: 145545 (14.90%) aligned discordantly 1 time ---- 831368 pairs aligned 0 times concordantly or discordantly; of these: 1662736 mates make up the pairs; of these: 1051407 (63.23%) aligned 0 times 325335 (19.57%) aligned exactly 1 time 285994 (17.20%) aligned >1 times 96.78% overall alignment rate Time searching: 00:31:08 Overall time: 00:31:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 346544 / 15453631 = 0.0224 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:57:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:57:41: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:57:41: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:57:47: 1000000 INFO @ Tue, 02 Aug 2022 15:57:53: 2000000 INFO @ Tue, 02 Aug 2022 15:57:59: 3000000 INFO @ Tue, 02 Aug 2022 15:58:05: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:58:10: 5000000 INFO @ Tue, 02 Aug 2022 15:58:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:58:10: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:58:10: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:58:16: 1000000 INFO @ Tue, 02 Aug 2022 15:58:16: 6000000 INFO @ Tue, 02 Aug 2022 15:58:22: 2000000 INFO @ Tue, 02 Aug 2022 15:58:22: 7000000 INFO @ Tue, 02 Aug 2022 15:58:28: 3000000 INFO @ Tue, 02 Aug 2022 15:58:29: 8000000 INFO @ Tue, 02 Aug 2022 15:58:34: 4000000 INFO @ Tue, 02 Aug 2022 15:58:35: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:58:40: 5000000 INFO @ Tue, 02 Aug 2022 15:58:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:58:41: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:58:41: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:58:41: 10000000 INFO @ Tue, 02 Aug 2022 15:58:45: 6000000 INFO @ Tue, 02 Aug 2022 15:58:47: 1000000 INFO @ Tue, 02 Aug 2022 15:58:47: 11000000 INFO @ Tue, 02 Aug 2022 15:58:51: 7000000 INFO @ Tue, 02 Aug 2022 15:58:52: 2000000 INFO @ Tue, 02 Aug 2022 15:58:52: 12000000 INFO @ Tue, 02 Aug 2022 15:58:57: 8000000 INFO @ Tue, 02 Aug 2022 15:58:58: 3000000 INFO @ Tue, 02 Aug 2022 15:58:58: 13000000 INFO @ Tue, 02 Aug 2022 15:59:03: 9000000 INFO @ Tue, 02 Aug 2022 15:59:03: 4000000 INFO @ Tue, 02 Aug 2022 15:59:04: 14000000 INFO @ Tue, 02 Aug 2022 15:59:09: 10000000 INFO @ Tue, 02 Aug 2022 15:59:09: 5000000 INFO @ Tue, 02 Aug 2022 15:59:10: 15000000 INFO @ Tue, 02 Aug 2022 15:59:14: 11000000 INFO @ Tue, 02 Aug 2022 15:59:15: 6000000 INFO @ Tue, 02 Aug 2022 15:59:16: 16000000 INFO @ Tue, 02 Aug 2022 15:59:20: 12000000 INFO @ Tue, 02 Aug 2022 15:59:21: 7000000 INFO @ Tue, 02 Aug 2022 15:59:22: 17000000 INFO @ Tue, 02 Aug 2022 15:59:26: 13000000 INFO @ Tue, 02 Aug 2022 15:59:27: 8000000 INFO @ Tue, 02 Aug 2022 15:59:28: 18000000 INFO @ Tue, 02 Aug 2022 15:59:31: 14000000 INFO @ Tue, 02 Aug 2022 15:59:32: 9000000 INFO @ Tue, 02 Aug 2022 15:59:33: 19000000 INFO @ Tue, 02 Aug 2022 15:59:37: 15000000 INFO @ Tue, 02 Aug 2022 15:59:38: 10000000 INFO @ Tue, 02 Aug 2022 15:59:39: 20000000 INFO @ Tue, 02 Aug 2022 15:59:43: 16000000 INFO @ Tue, 02 Aug 2022 15:59:43: 11000000 INFO @ Tue, 02 Aug 2022 15:59:45: 21000000 INFO @ Tue, 02 Aug 2022 15:59:48: 17000000 INFO @ Tue, 02 Aug 2022 15:59:49: 12000000 INFO @ Tue, 02 Aug 2022 15:59:51: 22000000 INFO @ Tue, 02 Aug 2022 15:59:54: 18000000 INFO @ Tue, 02 Aug 2022 15:59:55: 13000000 INFO @ Tue, 02 Aug 2022 15:59:57: 23000000 INFO @ Tue, 02 Aug 2022 15:59:59: 19000000 INFO @ Tue, 02 Aug 2022 16:00:00: 14000000 INFO @ Tue, 02 Aug 2022 16:00:03: 24000000 INFO @ Tue, 02 Aug 2022 16:00:05: 20000000 INFO @ Tue, 02 Aug 2022 16:00:06: 15000000 INFO @ Tue, 02 Aug 2022 16:00:09: 25000000 INFO @ Tue, 02 Aug 2022 16:00:11: 21000000 INFO @ Tue, 02 Aug 2022 16:00:11: 16000000 INFO @ Tue, 02 Aug 2022 16:00:15: 26000000 INFO @ Tue, 02 Aug 2022 16:00:16: 22000000 INFO @ Tue, 02 Aug 2022 16:00:17: 17000000 INFO @ Tue, 02 Aug 2022 16:00:21: 27000000 INFO @ Tue, 02 Aug 2022 16:00:22: 23000000 INFO @ Tue, 02 Aug 2022 16:00:22: 18000000 INFO @ Tue, 02 Aug 2022 16:00:27: 28000000 INFO @ Tue, 02 Aug 2022 16:00:28: 24000000 INFO @ Tue, 02 Aug 2022 16:00:28: 19000000 INFO @ Tue, 02 Aug 2022 16:00:33: 29000000 INFO @ Tue, 02 Aug 2022 16:00:33: 20000000 INFO @ Tue, 02 Aug 2022 16:00:33: 25000000 INFO @ Tue, 02 Aug 2022 16:00:39: 30000000 INFO @ Tue, 02 Aug 2022 16:00:39: 21000000 INFO @ Tue, 02 Aug 2022 16:00:39: 26000000 INFO @ Tue, 02 Aug 2022 16:00:44: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:00:44: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:00:44: #1 total tags in treatment: 15000676 INFO @ Tue, 02 Aug 2022 16:00:44: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:00:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:00:44: #1 tags after filtering in treatment: 14101894 INFO @ Tue, 02 Aug 2022 16:00:44: #1 Redundant rate of treatment: 0.06 INFO @ Tue, 02 Aug 2022 16:00:44: #1 finished! INFO @ Tue, 02 Aug 2022 16:00:44: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:00:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:00:45: 22000000 INFO @ Tue, 02 Aug 2022 16:00:45: 27000000 INFO @ Tue, 02 Aug 2022 16:00:45: #2 number of paired peaks: 78 WARNING @ Tue, 02 Aug 2022 16:00:45: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:00:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:00:50: 23000000 INFO @ Tue, 02 Aug 2022 16:00:51: 28000000 INFO @ Tue, 02 Aug 2022 16:00:56: 24000000 INFO @ Tue, 02 Aug 2022 16:00:56: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:01:02: 30000000 INFO @ Tue, 02 Aug 2022 16:01:02: 25000000 INFO @ Tue, 02 Aug 2022 16:01:07: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:01:07: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:01:07: #1 total tags in treatment: 15000676 INFO @ Tue, 02 Aug 2022 16:01:07: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:01:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:01:07: #1 tags after filtering in treatment: 14101894 INFO @ Tue, 02 Aug 2022 16:01:07: #1 Redundant rate of treatment: 0.06 INFO @ Tue, 02 Aug 2022 16:01:07: #1 finished! INFO @ Tue, 02 Aug 2022 16:01:07: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:01:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:01:08: 26000000 INFO @ Tue, 02 Aug 2022 16:01:08: #2 number of paired peaks: 78 WARNING @ Tue, 02 Aug 2022 16:01:08: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:01:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:01:13: 27000000 INFO @ Tue, 02 Aug 2022 16:01:19: 28000000 INFO @ Tue, 02 Aug 2022 16:01:25: 29000000 INFO @ Tue, 02 Aug 2022 16:01:30: 30000000 INFO @ Tue, 02 Aug 2022 16:01:35: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:01:35: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:01:35: #1 total tags in treatment: 15000676 INFO @ Tue, 02 Aug 2022 16:01:35: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:01:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:01:35: #1 tags after filtering in treatment: 14101894 INFO @ Tue, 02 Aug 2022 16:01:35: #1 Redundant rate of treatment: 0.06 INFO @ Tue, 02 Aug 2022 16:01:35: #1 finished! INFO @ Tue, 02 Aug 2022 16:01:35: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:01:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:01:36: #2 number of paired peaks: 78 WARNING @ Tue, 02 Aug 2022 16:01:36: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:01:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518317/SRX9518317.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。