Job ID = 16439458 SRX = SRX9518316 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 19580528 spots for SRR13070799/SRR13070799.sra Written 19580528 spots for SRR13070799/SRR13070799.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439838 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:57:15 19580528 reads; of these: 19580528 (100.00%) were paired; of these: 1184644 (6.05%) aligned concordantly 0 times 14456407 (73.83%) aligned concordantly exactly 1 time 3939477 (20.12%) aligned concordantly >1 times ---- 1184644 pairs aligned concordantly 0 times; of these: 250973 (21.19%) aligned discordantly 1 time ---- 933671 pairs aligned 0 times concordantly or discordantly; of these: 1867342 mates make up the pairs; of these: 1217971 (65.22%) aligned 0 times 341089 (18.27%) aligned exactly 1 time 308282 (16.51%) aligned >1 times 96.89% overall alignment rate Time searching: 00:57:15 Overall time: 00:57:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 138766 / 18603967 = 0.0075 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:34:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:34:26: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:34:26: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:34:40: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:34:53: 2000000 INFO @ Tue, 02 Aug 2022 16:34:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:34:55: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:34:55: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:35:06: 3000000 INFO @ Tue, 02 Aug 2022 16:35:09: 1000000 INFO @ Tue, 02 Aug 2022 16:35:18: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:35:22: 2000000 INFO @ Tue, 02 Aug 2022 16:35:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:35:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:35:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:35:31: 5000000 INFO @ Tue, 02 Aug 2022 16:35:36: 3000000 INFO @ Tue, 02 Aug 2022 16:35:40: 1000000 INFO @ Tue, 02 Aug 2022 16:35:44: 6000000 INFO @ Tue, 02 Aug 2022 16:35:49: 4000000 INFO @ Tue, 02 Aug 2022 16:35:53: 2000000 INFO @ Tue, 02 Aug 2022 16:35:57: 7000000 INFO @ Tue, 02 Aug 2022 16:36:03: 5000000 INFO @ Tue, 02 Aug 2022 16:36:07: 3000000 INFO @ Tue, 02 Aug 2022 16:36:10: 8000000 INFO @ Tue, 02 Aug 2022 16:36:16: 6000000 INFO @ Tue, 02 Aug 2022 16:36:21: 4000000 INFO @ Tue, 02 Aug 2022 16:36:23: 9000000 INFO @ Tue, 02 Aug 2022 16:36:29: 7000000 INFO @ Tue, 02 Aug 2022 16:36:34: 5000000 INFO @ Tue, 02 Aug 2022 16:36:36: 10000000 INFO @ Tue, 02 Aug 2022 16:36:42: 8000000 INFO @ Tue, 02 Aug 2022 16:36:48: 6000000 INFO @ Tue, 02 Aug 2022 16:36:50: 11000000 INFO @ Tue, 02 Aug 2022 16:36:56: 9000000 INFO @ Tue, 02 Aug 2022 16:37:02: 7000000 INFO @ Tue, 02 Aug 2022 16:37:03: 12000000 INFO @ Tue, 02 Aug 2022 16:37:11: 10000000 INFO @ Tue, 02 Aug 2022 16:37:16: 13000000 INFO @ Tue, 02 Aug 2022 16:37:16: 8000000 INFO @ Tue, 02 Aug 2022 16:37:25: 11000000 INFO @ Tue, 02 Aug 2022 16:37:29: 9000000 INFO @ Tue, 02 Aug 2022 16:37:30: 14000000 INFO @ Tue, 02 Aug 2022 16:37:40: 12000000 INFO @ Tue, 02 Aug 2022 16:37:42: 10000000 INFO @ Tue, 02 Aug 2022 16:37:44: 15000000 INFO @ Tue, 02 Aug 2022 16:37:54: 13000000 INFO @ Tue, 02 Aug 2022 16:37:54: 11000000 INFO @ Tue, 02 Aug 2022 16:37:58: 16000000 INFO @ Tue, 02 Aug 2022 16:38:06: 12000000 INFO @ Tue, 02 Aug 2022 16:38:09: 14000000 INFO @ Tue, 02 Aug 2022 16:38:12: 17000000 INFO @ Tue, 02 Aug 2022 16:38:18: 13000000 INFO @ Tue, 02 Aug 2022 16:38:24: 15000000 INFO @ Tue, 02 Aug 2022 16:38:26: 18000000 INFO @ Tue, 02 Aug 2022 16:38:31: 14000000 INFO @ Tue, 02 Aug 2022 16:38:39: 19000000 INFO @ Tue, 02 Aug 2022 16:38:40: 16000000 INFO @ Tue, 02 Aug 2022 16:38:43: 15000000 INFO @ Tue, 02 Aug 2022 16:38:53: 20000000 INFO @ Tue, 02 Aug 2022 16:38:55: 16000000 INFO @ Tue, 02 Aug 2022 16:38:56: 17000000 INFO @ Tue, 02 Aug 2022 16:39:08: 21000000 INFO @ Tue, 02 Aug 2022 16:39:08: 17000000 INFO @ Tue, 02 Aug 2022 16:39:11: 18000000 INFO @ Tue, 02 Aug 2022 16:39:20: 18000000 INFO @ Tue, 02 Aug 2022 16:39:22: 22000000 INFO @ Tue, 02 Aug 2022 16:39:26: 19000000 INFO @ Tue, 02 Aug 2022 16:39:32: 19000000 INFO @ Tue, 02 Aug 2022 16:39:37: 23000000 INFO @ Tue, 02 Aug 2022 16:39:41: 20000000 INFO @ Tue, 02 Aug 2022 16:39:45: 20000000 INFO @ Tue, 02 Aug 2022 16:39:52: 24000000 INFO @ Tue, 02 Aug 2022 16:39:56: 21000000 INFO @ Tue, 02 Aug 2022 16:39:57: 21000000 INFO @ Tue, 02 Aug 2022 16:40:06: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:40:09: 22000000 INFO @ Tue, 02 Aug 2022 16:40:11: 22000000 INFO @ Tue, 02 Aug 2022 16:40:19: 26000000 INFO @ Tue, 02 Aug 2022 16:40:22: 23000000 INFO @ Tue, 02 Aug 2022 16:40:26: 23000000 INFO @ Tue, 02 Aug 2022 16:40:33: 27000000 INFO @ Tue, 02 Aug 2022 16:40:34: 24000000 INFO @ Tue, 02 Aug 2022 16:40:41: 24000000 INFO @ Tue, 02 Aug 2022 16:40:46: 25000000 INFO @ Tue, 02 Aug 2022 16:40:47: 28000000 INFO @ Tue, 02 Aug 2022 16:40:56: 25000000 INFO @ Tue, 02 Aug 2022 16:40:59: 26000000 INFO @ Tue, 02 Aug 2022 16:41:02: 29000000 INFO @ Tue, 02 Aug 2022 16:41:12: 26000000 INFO @ Tue, 02 Aug 2022 16:41:12: 27000000 INFO @ Tue, 02 Aug 2022 16:41:17: 30000000 INFO @ Tue, 02 Aug 2022 16:41:25: 28000000 INFO @ Tue, 02 Aug 2022 16:41:27: 27000000 INFO @ Tue, 02 Aug 2022 16:41:32: 31000000 INFO @ Tue, 02 Aug 2022 16:41:37: 29000000 INFO @ Tue, 02 Aug 2022 16:41:43: 28000000 INFO @ Tue, 02 Aug 2022 16:41:46: 32000000 INFO @ Tue, 02 Aug 2022 16:41:50: 30000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 16:42:00: 29000000 INFO @ Tue, 02 Aug 2022 16:42:02: 33000000 INFO @ Tue, 02 Aug 2022 16:42:03: 31000000 INFO @ Tue, 02 Aug 2022 16:42:15: 32000000 INFO @ Tue, 02 Aug 2022 16:42:16: 30000000 INFO @ Tue, 02 Aug 2022 16:42:17: 34000000 INFO @ Tue, 02 Aug 2022 16:42:27: 33000000 INFO @ Tue, 02 Aug 2022 16:42:31: 35000000 INFO @ Tue, 02 Aug 2022 16:42:32: 31000000 INFO @ Tue, 02 Aug 2022 16:42:40: 34000000 INFO @ Tue, 02 Aug 2022 16:42:46: 36000000 INFO @ Tue, 02 Aug 2022 16:42:48: 32000000 INFO @ Tue, 02 Aug 2022 16:42:52: 35000000 INFO @ Tue, 02 Aug 2022 16:43:01: 37000000 INFO @ Tue, 02 Aug 2022 16:43:04: 33000000 INFO @ Tue, 02 Aug 2022 16:43:05: 36000000 INFO @ Tue, 02 Aug 2022 16:43:11: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:43:11: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:43:11: #1 total tags in treatment: 18257804 INFO @ Tue, 02 Aug 2022 16:43:11: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:43:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:43:11: #1 tags after filtering in treatment: 17364442 INFO @ Tue, 02 Aug 2022 16:43:11: #1 Redundant rate of treatment: 0.05 INFO @ Tue, 02 Aug 2022 16:43:11: #1 finished! INFO @ Tue, 02 Aug 2022 16:43:11: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:43:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:43:13: #2 number of paired peaks: 13 WARNING @ Tue, 02 Aug 2022 16:43:13: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:43:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:43:16: 37000000 INFO @ Tue, 02 Aug 2022 16:43:19: 34000000 INFO @ Tue, 02 Aug 2022 16:43:24: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:43:24: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:43:24: #1 total tags in treatment: 18257804 INFO @ Tue, 02 Aug 2022 16:43:24: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:43:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:43:25: #1 tags after filtering in treatment: 17364442 INFO @ Tue, 02 Aug 2022 16:43:25: #1 Redundant rate of treatment: 0.05 INFO @ Tue, 02 Aug 2022 16:43:25: #1 finished! INFO @ Tue, 02 Aug 2022 16:43:25: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:43:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:43:26: #2 number of paired peaks: 13 WARNING @ Tue, 02 Aug 2022 16:43:26: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:43:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:43:33: 35000000 INFO @ Tue, 02 Aug 2022 16:43:47: 36000000 INFO @ Tue, 02 Aug 2022 16:44:01: 37000000 INFO @ Tue, 02 Aug 2022 16:44:10: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:44:10: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:44:10: #1 total tags in treatment: 18257804 INFO @ Tue, 02 Aug 2022 16:44:10: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:44:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:44:11: #1 tags after filtering in treatment: 17364442 INFO @ Tue, 02 Aug 2022 16:44:11: #1 Redundant rate of treatment: 0.05 INFO @ Tue, 02 Aug 2022 16:44:11: #1 finished! INFO @ Tue, 02 Aug 2022 16:44:11: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:44:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:44:12: #2 number of paired peaks: 13 WARNING @ Tue, 02 Aug 2022 16:44:12: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:44:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518316/SRX9518316.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling