Job ID = 14172142 SRX = SRX9103831 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 55945969 spots for SRR12621031/SRR12621031.sra Written 55945969 spots for SRR12621031/SRR12621031.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172879 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:58:41 55945969 reads; of these: 55945969 (100.00%) were paired; of these: 17649474 (31.55%) aligned concordantly 0 times 20006691 (35.76%) aligned concordantly exactly 1 time 18289804 (32.69%) aligned concordantly >1 times ---- 17649474 pairs aligned concordantly 0 times; of these: 3727428 (21.12%) aligned discordantly 1 time ---- 13922046 pairs aligned 0 times concordantly or discordantly; of these: 27844092 mates make up the pairs; of these: 22174811 (79.64%) aligned 0 times 1374956 (4.94%) aligned exactly 1 time 4294325 (15.42%) aligned >1 times 80.18% overall alignment rate Time searching: 00:58:41 Overall time: 00:58:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 14159863 / 41452056 = 0.3416 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:51:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:51:47: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:51:47: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:51:52: 1000000 INFO @ Sat, 11 Dec 2021 15:51:57: 2000000 INFO @ Sat, 11 Dec 2021 15:52:02: 3000000 INFO @ Sat, 11 Dec 2021 15:52:07: 4000000 INFO @ Sat, 11 Dec 2021 15:52:12: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:52:16: 6000000 INFO @ Sat, 11 Dec 2021 15:52:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:52:17: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:52:17: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:52:22: 7000000 INFO @ Sat, 11 Dec 2021 15:52:23: 1000000 INFO @ Sat, 11 Dec 2021 15:52:27: 8000000 INFO @ Sat, 11 Dec 2021 15:52:28: 2000000 INFO @ Sat, 11 Dec 2021 15:52:33: 9000000 INFO @ Sat, 11 Dec 2021 15:52:33: 3000000 INFO @ Sat, 11 Dec 2021 15:52:38: 10000000 INFO @ Sat, 11 Dec 2021 15:52:39: 4000000 INFO @ Sat, 11 Dec 2021 15:52:43: 11000000 INFO @ Sat, 11 Dec 2021 15:52:44: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:52:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:52:47: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:52:47: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:52:49: 12000000 INFO @ Sat, 11 Dec 2021 15:52:50: 6000000 INFO @ Sat, 11 Dec 2021 15:52:53: 1000000 INFO @ Sat, 11 Dec 2021 15:52:54: 13000000 INFO @ Sat, 11 Dec 2021 15:52:55: 7000000 INFO @ Sat, 11 Dec 2021 15:52:58: 2000000 INFO @ Sat, 11 Dec 2021 15:53:00: 14000000 INFO @ Sat, 11 Dec 2021 15:53:01: 8000000 INFO @ Sat, 11 Dec 2021 15:53:04: 3000000 INFO @ Sat, 11 Dec 2021 15:53:05: 15000000 INFO @ Sat, 11 Dec 2021 15:53:06: 9000000 INFO @ Sat, 11 Dec 2021 15:53:09: 4000000 INFO @ Sat, 11 Dec 2021 15:53:11: 16000000 INFO @ Sat, 11 Dec 2021 15:53:11: 10000000 INFO @ Sat, 11 Dec 2021 15:53:14: 5000000 INFO @ Sat, 11 Dec 2021 15:53:16: 17000000 INFO @ Sat, 11 Dec 2021 15:53:17: 11000000 INFO @ Sat, 11 Dec 2021 15:53:20: 6000000 INFO @ Sat, 11 Dec 2021 15:53:21: 18000000 INFO @ Sat, 11 Dec 2021 15:53:22: 12000000 INFO @ Sat, 11 Dec 2021 15:53:25: 7000000 INFO @ Sat, 11 Dec 2021 15:53:27: 19000000 INFO @ Sat, 11 Dec 2021 15:53:28: 13000000 INFO @ Sat, 11 Dec 2021 15:53:31: 8000000 INFO @ Sat, 11 Dec 2021 15:53:32: 20000000 INFO @ Sat, 11 Dec 2021 15:53:34: 14000000 INFO @ Sat, 11 Dec 2021 15:53:36: 9000000 INFO @ Sat, 11 Dec 2021 15:53:38: 21000000 INFO @ Sat, 11 Dec 2021 15:53:39: 15000000 INFO @ Sat, 11 Dec 2021 15:53:42: 10000000 INFO @ Sat, 11 Dec 2021 15:53:43: 22000000 INFO @ Sat, 11 Dec 2021 15:53:44: 16000000 INFO @ Sat, 11 Dec 2021 15:53:47: 11000000 INFO @ Sat, 11 Dec 2021 15:53:48: 23000000 INFO @ Sat, 11 Dec 2021 15:53:50: 17000000 INFO @ Sat, 11 Dec 2021 15:53:53: 12000000 INFO @ Sat, 11 Dec 2021 15:53:54: 24000000 INFO @ Sat, 11 Dec 2021 15:53:56: 18000000 INFO @ Sat, 11 Dec 2021 15:53:58: 13000000 INFO @ Sat, 11 Dec 2021 15:53:59: 25000000 INFO @ Sat, 11 Dec 2021 15:54:01: 19000000 INFO @ Sat, 11 Dec 2021 15:54:03: 14000000 INFO @ Sat, 11 Dec 2021 15:54:05: 26000000 INFO @ Sat, 11 Dec 2021 15:54:06: 20000000 INFO @ Sat, 11 Dec 2021 15:54:09: 15000000 INFO @ Sat, 11 Dec 2021 15:54:10: 27000000 INFO @ Sat, 11 Dec 2021 15:54:12: 21000000 INFO @ Sat, 11 Dec 2021 15:54:14: 16000000 INFO @ Sat, 11 Dec 2021 15:54:15: 28000000 INFO @ Sat, 11 Dec 2021 15:54:17: 22000000 INFO @ Sat, 11 Dec 2021 15:54:20: 17000000 INFO @ Sat, 11 Dec 2021 15:54:21: 29000000 INFO @ Sat, 11 Dec 2021 15:54:23: 23000000 INFO @ Sat, 11 Dec 2021 15:54:25: 18000000 INFO @ Sat, 11 Dec 2021 15:54:26: 30000000 INFO @ Sat, 11 Dec 2021 15:54:28: 24000000 INFO @ Sat, 11 Dec 2021 15:54:30: 19000000 INFO @ Sat, 11 Dec 2021 15:54:32: 31000000 INFO @ Sat, 11 Dec 2021 15:54:33: 25000000 INFO @ Sat, 11 Dec 2021 15:54:36: 20000000 INFO @ Sat, 11 Dec 2021 15:54:37: 32000000 INFO @ Sat, 11 Dec 2021 15:54:39: 26000000 INFO @ Sat, 11 Dec 2021 15:54:41: 21000000 INFO @ Sat, 11 Dec 2021 15:54:42: 33000000 INFO @ Sat, 11 Dec 2021 15:54:44: 27000000 INFO @ Sat, 11 Dec 2021 15:54:47: 22000000 INFO @ Sat, 11 Dec 2021 15:54:48: 34000000 INFO @ Sat, 11 Dec 2021 15:54:50: 28000000 INFO @ Sat, 11 Dec 2021 15:54:52: 23000000 INFO @ Sat, 11 Dec 2021 15:54:54: 35000000 INFO @ Sat, 11 Dec 2021 15:54:55: 29000000 INFO @ Sat, 11 Dec 2021 15:54:57: 24000000 INFO @ Sat, 11 Dec 2021 15:54:59: 36000000 INFO @ Sat, 11 Dec 2021 15:55:00: 30000000 INFO @ Sat, 11 Dec 2021 15:55:03: 25000000 INFO @ Sat, 11 Dec 2021 15:55:04: 37000000 INFO @ Sat, 11 Dec 2021 15:55:06: 31000000 INFO @ Sat, 11 Dec 2021 15:55:08: 26000000 INFO @ Sat, 11 Dec 2021 15:55:10: 38000000 INFO @ Sat, 11 Dec 2021 15:55:11: 32000000 INFO @ Sat, 11 Dec 2021 15:55:14: 27000000 INFO @ Sat, 11 Dec 2021 15:55:15: 39000000 INFO @ Sat, 11 Dec 2021 15:55:17: 33000000 INFO @ Sat, 11 Dec 2021 15:55:19: 28000000 INFO @ Sat, 11 Dec 2021 15:55:21: 40000000 INFO @ Sat, 11 Dec 2021 15:55:22: 34000000 INFO @ Sat, 11 Dec 2021 15:55:24: 29000000 INFO @ Sat, 11 Dec 2021 15:55:26: 41000000 INFO @ Sat, 11 Dec 2021 15:55:27: 35000000 INFO @ Sat, 11 Dec 2021 15:55:30: 30000000 INFO @ Sat, 11 Dec 2021 15:55:31: 42000000 INFO @ Sat, 11 Dec 2021 15:55:33: 36000000 INFO @ Sat, 11 Dec 2021 15:55:35: 31000000 INFO @ Sat, 11 Dec 2021 15:55:36: 43000000 INFO @ Sat, 11 Dec 2021 15:55:38: 37000000 INFO @ Sat, 11 Dec 2021 15:55:40: 32000000 INFO @ Sat, 11 Dec 2021 15:55:41: 44000000 INFO @ Sat, 11 Dec 2021 15:55:43: 38000000 INFO @ Sat, 11 Dec 2021 15:55:46: 33000000 INFO @ Sat, 11 Dec 2021 15:55:46: 45000000 INFO @ Sat, 11 Dec 2021 15:55:49: 39000000 INFO @ Sat, 11 Dec 2021 15:55:51: 34000000 INFO @ Sat, 11 Dec 2021 15:55:52: 46000000 INFO @ Sat, 11 Dec 2021 15:55:54: 40000000 INFO @ Sat, 11 Dec 2021 15:55:56: 35000000 INFO @ Sat, 11 Dec 2021 15:55:57: 47000000 INFO @ Sat, 11 Dec 2021 15:55:59: 41000000 INFO @ Sat, 11 Dec 2021 15:56:02: 36000000 INFO @ Sat, 11 Dec 2021 15:56:03: 48000000 INFO @ Sat, 11 Dec 2021 15:56:04: 42000000 INFO @ Sat, 11 Dec 2021 15:56:07: 37000000 INFO @ Sat, 11 Dec 2021 15:56:08: 49000000 INFO @ Sat, 11 Dec 2021 15:56:09: 43000000 INFO @ Sat, 11 Dec 2021 15:56:12: 38000000 INFO @ Sat, 11 Dec 2021 15:56:13: 50000000 INFO @ Sat, 11 Dec 2021 15:56:14: 44000000 INFO @ Sat, 11 Dec 2021 15:56:18: 39000000 INFO @ Sat, 11 Dec 2021 15:56:19: 51000000 INFO @ Sat, 11 Dec 2021 15:56:20: 45000000 INFO @ Sat, 11 Dec 2021 15:56:23: 40000000 INFO @ Sat, 11 Dec 2021 15:56:24: 52000000 INFO @ Sat, 11 Dec 2021 15:56:25: 46000000 INFO @ Sat, 11 Dec 2021 15:56:28: 41000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 15:56:29: 53000000 INFO @ Sat, 11 Dec 2021 15:56:30: 47000000 INFO @ Sat, 11 Dec 2021 15:56:33: 42000000 INFO @ Sat, 11 Dec 2021 15:56:35: 54000000 INFO @ Sat, 11 Dec 2021 15:56:36: 48000000 INFO @ Sat, 11 Dec 2021 15:56:39: 43000000 INFO @ Sat, 11 Dec 2021 15:56:41: 55000000 INFO @ Sat, 11 Dec 2021 15:56:42: 49000000 INFO @ Sat, 11 Dec 2021 15:56:44: 44000000 INFO @ Sat, 11 Dec 2021 15:56:46: 56000000 INFO @ Sat, 11 Dec 2021 15:56:47: 50000000 INFO @ Sat, 11 Dec 2021 15:56:49: 45000000 INFO @ Sat, 11 Dec 2021 15:56:52: 57000000 INFO @ Sat, 11 Dec 2021 15:56:53: 51000000 INFO @ Sat, 11 Dec 2021 15:56:55: 46000000 INFO @ Sat, 11 Dec 2021 15:56:57: 58000000 INFO @ Sat, 11 Dec 2021 15:56:58: 52000000 INFO @ Sat, 11 Dec 2021 15:57:00: 47000000 INFO @ Sat, 11 Dec 2021 15:57:03: 59000000 INFO @ Sat, 11 Dec 2021 15:57:04: 53000000 INFO @ Sat, 11 Dec 2021 15:57:06: 48000000 INFO @ Sat, 11 Dec 2021 15:57:08: 60000000 INFO @ Sat, 11 Dec 2021 15:57:09: 54000000 INFO @ Sat, 11 Dec 2021 15:57:11: 49000000 INFO @ Sat, 11 Dec 2021 15:57:13: 61000000 INFO @ Sat, 11 Dec 2021 15:57:15: 55000000 INFO @ Sat, 11 Dec 2021 15:57:16: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:57:16: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:57:16: #1 total tags in treatment: 24563294 INFO @ Sat, 11 Dec 2021 15:57:16: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:57:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:57:16: #1 tags after filtering in treatment: 18433422 INFO @ Sat, 11 Dec 2021 15:57:16: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 11 Dec 2021 15:57:16: #1 finished! INFO @ Sat, 11 Dec 2021 15:57:16: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:57:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:57:17: 50000000 INFO @ Sat, 11 Dec 2021 15:57:17: #2 number of paired peaks: 127 WARNING @ Sat, 11 Dec 2021 15:57:17: Fewer paired peaks (127) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 127 pairs to build model! INFO @ Sat, 11 Dec 2021 15:57:17: start model_add_line... INFO @ Sat, 11 Dec 2021 15:57:17: start X-correlation... INFO @ Sat, 11 Dec 2021 15:57:17: end of X-cor INFO @ Sat, 11 Dec 2021 15:57:17: #2 finished! INFO @ Sat, 11 Dec 2021 15:57:17: #2 predicted fragment length is 1 bps INFO @ Sat, 11 Dec 2021 15:57:17: #2 alternative fragment length(s) may be 1,10,57,103,144,224,262,317,428,432,496,574 bps INFO @ Sat, 11 Dec 2021 15:57:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.05_model.r WARNING @ Sat, 11 Dec 2021 15:57:18: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 15:57:18: #2 You may need to consider one of the other alternative d(s): 1,10,57,103,144,224,262,317,428,432,496,574 WARNING @ Sat, 11 Dec 2021 15:57:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 15:57:18: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:57:18: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:57:20: 56000000 INFO @ Sat, 11 Dec 2021 15:57:22: 51000000 INFO @ Sat, 11 Dec 2021 15:57:25: 57000000 INFO @ Sat, 11 Dec 2021 15:57:28: 52000000 INFO @ Sat, 11 Dec 2021 15:57:31: 58000000 INFO @ Sat, 11 Dec 2021 15:57:33: 53000000 INFO @ Sat, 11 Dec 2021 15:57:36: 59000000 INFO @ Sat, 11 Dec 2021 15:57:38: 54000000 INFO @ Sat, 11 Dec 2021 15:57:41: 60000000 INFO @ Sat, 11 Dec 2021 15:57:44: 55000000 INFO @ Sat, 11 Dec 2021 15:57:47: 61000000 INFO @ Sat, 11 Dec 2021 15:57:49: 56000000 INFO @ Sat, 11 Dec 2021 15:57:49: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:57:49: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:57:49: #1 total tags in treatment: 24563294 INFO @ Sat, 11 Dec 2021 15:57:49: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:57:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:57:49: #1 tags after filtering in treatment: 18433422 INFO @ Sat, 11 Dec 2021 15:57:49: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 11 Dec 2021 15:57:49: #1 finished! INFO @ Sat, 11 Dec 2021 15:57:49: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:57:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:57:49: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:57:51: #2 number of paired peaks: 127 WARNING @ Sat, 11 Dec 2021 15:57:51: Fewer paired peaks (127) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 127 pairs to build model! INFO @ Sat, 11 Dec 2021 15:57:51: start model_add_line... INFO @ Sat, 11 Dec 2021 15:57:51: start X-correlation... INFO @ Sat, 11 Dec 2021 15:57:51: end of X-cor INFO @ Sat, 11 Dec 2021 15:57:51: #2 finished! INFO @ Sat, 11 Dec 2021 15:57:51: #2 predicted fragment length is 1 bps INFO @ Sat, 11 Dec 2021 15:57:51: #2 alternative fragment length(s) may be 1,10,57,103,144,224,262,317,428,432,496,574 bps INFO @ Sat, 11 Dec 2021 15:57:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.10_model.r WARNING @ Sat, 11 Dec 2021 15:57:51: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 15:57:51: #2 You may need to consider one of the other alternative d(s): 1,10,57,103,144,224,262,317,428,432,496,574 WARNING @ Sat, 11 Dec 2021 15:57:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 15:57:51: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:57:51: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 15:57:54: 57000000 INFO @ Sat, 11 Dec 2021 15:57:59: 58000000 INFO @ Sat, 11 Dec 2021 15:58:04: 59000000 INFO @ Sat, 11 Dec 2021 15:58:04: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.05_peaks.xls INFO @ Sat, 11 Dec 2021 15:58:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:58:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.05_summits.bed INFO @ Sat, 11 Dec 2021 15:58:04: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:58:09: 60000000 INFO @ Sat, 11 Dec 2021 15:58:14: 61000000 INFO @ Sat, 11 Dec 2021 15:58:16: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:58:16: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:58:16: #1 total tags in treatment: 24563294 INFO @ Sat, 11 Dec 2021 15:58:16: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:58:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:58:17: #1 tags after filtering in treatment: 18433422 INFO @ Sat, 11 Dec 2021 15:58:17: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 11 Dec 2021 15:58:17: #1 finished! INFO @ Sat, 11 Dec 2021 15:58:17: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:58:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:58:18: #2 number of paired peaks: 127 WARNING @ Sat, 11 Dec 2021 15:58:18: Fewer paired peaks (127) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 127 pairs to build model! INFO @ Sat, 11 Dec 2021 15:58:18: start model_add_line... INFO @ Sat, 11 Dec 2021 15:58:18: start X-correlation... INFO @ Sat, 11 Dec 2021 15:58:18: end of X-cor INFO @ Sat, 11 Dec 2021 15:58:18: #2 finished! INFO @ Sat, 11 Dec 2021 15:58:18: #2 predicted fragment length is 1 bps INFO @ Sat, 11 Dec 2021 15:58:18: #2 alternative fragment length(s) may be 1,10,57,103,144,224,262,317,428,432,496,574 bps INFO @ Sat, 11 Dec 2021 15:58:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.20_model.r WARNING @ Sat, 11 Dec 2021 15:58:18: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 15:58:18: #2 You may need to consider one of the other alternative d(s): 1,10,57,103,144,224,262,317,428,432,496,574 WARNING @ Sat, 11 Dec 2021 15:58:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 15:58:18: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:58:18: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:58:21: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:58:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.10_peaks.xls INFO @ Sat, 11 Dec 2021 15:58:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:58:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.10_summits.bed INFO @ Sat, 11 Dec 2021 15:58:36: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:58:48: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:59:03: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.20_peaks.xls INFO @ Sat, 11 Dec 2021 15:59:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:59:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9103831/SRX9103831.20_summits.bed INFO @ Sat, 11 Dec 2021 15:59:03: Done! pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling