Job ID = 14172136 SRX = SRX9103828 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 50948328 spots for SRR12621028/SRR12621028.sra Written 50948328 spots for SRR12621028/SRR12621028.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172948 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:18:29 50948328 reads; of these: 50948328 (100.00%) were paired; of these: 15998273 (31.40%) aligned concordantly 0 times 18583726 (36.48%) aligned concordantly exactly 1 time 16366329 (32.12%) aligned concordantly >1 times ---- 15998273 pairs aligned concordantly 0 times; of these: 3988196 (24.93%) aligned discordantly 1 time ---- 12010077 pairs aligned 0 times concordantly or discordantly; of these: 24020154 mates make up the pairs; of these: 17942479 (74.70%) aligned 0 times 1552195 (6.46%) aligned exactly 1 time 4525480 (18.84%) aligned >1 times 82.39% overall alignment rate Time searching: 01:18:30 Overall time: 01:18:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 14019887 / 38349527 = 0.3656 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:15:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:15:22: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:15:22: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:15:31: 1000000 INFO @ Sat, 11 Dec 2021 16:15:41: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:15:50: 3000000 INFO @ Sat, 11 Dec 2021 16:15:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:15:51: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:15:51: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:16:00: 1000000 INFO @ Sat, 11 Dec 2021 16:16:00: 4000000 INFO @ Sat, 11 Dec 2021 16:16:08: 2000000 INFO @ Sat, 11 Dec 2021 16:16:10: 5000000 INFO @ Sat, 11 Dec 2021 16:16:16: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:16:19: 6000000 INFO @ Sat, 11 Dec 2021 16:16:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:16:21: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:16:21: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:16:24: 4000000 INFO @ Sat, 11 Dec 2021 16:16:29: 7000000 INFO @ Sat, 11 Dec 2021 16:16:31: 1000000 INFO @ Sat, 11 Dec 2021 16:16:33: 5000000 INFO @ Sat, 11 Dec 2021 16:16:38: 8000000 INFO @ Sat, 11 Dec 2021 16:16:40: 2000000 INFO @ Sat, 11 Dec 2021 16:16:41: 6000000 INFO @ Sat, 11 Dec 2021 16:16:48: 9000000 INFO @ Sat, 11 Dec 2021 16:16:49: 3000000 INFO @ Sat, 11 Dec 2021 16:16:50: 7000000 INFO @ Sat, 11 Dec 2021 16:16:58: 10000000 INFO @ Sat, 11 Dec 2021 16:16:58: 8000000 INFO @ Sat, 11 Dec 2021 16:16:59: 4000000 INFO @ Sat, 11 Dec 2021 16:17:07: 9000000 INFO @ Sat, 11 Dec 2021 16:17:07: 11000000 INFO @ Sat, 11 Dec 2021 16:17:08: 5000000 INFO @ Sat, 11 Dec 2021 16:17:15: 10000000 INFO @ Sat, 11 Dec 2021 16:17:17: 12000000 INFO @ Sat, 11 Dec 2021 16:17:17: 6000000 INFO @ Sat, 11 Dec 2021 16:17:24: 11000000 INFO @ Sat, 11 Dec 2021 16:17:27: 7000000 INFO @ Sat, 11 Dec 2021 16:17:27: 13000000 INFO @ Sat, 11 Dec 2021 16:17:32: 12000000 INFO @ Sat, 11 Dec 2021 16:17:36: 8000000 INFO @ Sat, 11 Dec 2021 16:17:38: 14000000 INFO @ Sat, 11 Dec 2021 16:17:40: 13000000 INFO @ Sat, 11 Dec 2021 16:17:46: 9000000 INFO @ Sat, 11 Dec 2021 16:17:49: 15000000 INFO @ Sat, 11 Dec 2021 16:17:49: 14000000 INFO @ Sat, 11 Dec 2021 16:17:55: 10000000 INFO @ Sat, 11 Dec 2021 16:17:57: 15000000 INFO @ Sat, 11 Dec 2021 16:17:58: 16000000 INFO @ Sat, 11 Dec 2021 16:18:05: 11000000 INFO @ Sat, 11 Dec 2021 16:18:06: 16000000 INFO @ Sat, 11 Dec 2021 16:18:08: 17000000 INFO @ Sat, 11 Dec 2021 16:18:14: 17000000 INFO @ Sat, 11 Dec 2021 16:18:15: 12000000 INFO @ Sat, 11 Dec 2021 16:18:18: 18000000 INFO @ Sat, 11 Dec 2021 16:18:22: 18000000 INFO @ Sat, 11 Dec 2021 16:18:24: 13000000 INFO @ Sat, 11 Dec 2021 16:18:27: 19000000 INFO @ Sat, 11 Dec 2021 16:18:31: 19000000 INFO @ Sat, 11 Dec 2021 16:18:34: 14000000 INFO @ Sat, 11 Dec 2021 16:18:37: 20000000 INFO @ Sat, 11 Dec 2021 16:18:40: 20000000 INFO @ Sat, 11 Dec 2021 16:18:43: 15000000 INFO @ Sat, 11 Dec 2021 16:18:46: 21000000 INFO @ Sat, 11 Dec 2021 16:18:49: 21000000 INFO @ Sat, 11 Dec 2021 16:18:53: 16000000 INFO @ Sat, 11 Dec 2021 16:18:56: 22000000 INFO @ Sat, 11 Dec 2021 16:18:57: 22000000 INFO @ Sat, 11 Dec 2021 16:19:02: 17000000 INFO @ Sat, 11 Dec 2021 16:19:06: 23000000 INFO @ Sat, 11 Dec 2021 16:19:06: 23000000 INFO @ Sat, 11 Dec 2021 16:19:12: 18000000 INFO @ Sat, 11 Dec 2021 16:19:15: 24000000 INFO @ Sat, 11 Dec 2021 16:19:15: 24000000 INFO @ Sat, 11 Dec 2021 16:19:21: 19000000 INFO @ Sat, 11 Dec 2021 16:19:24: 25000000 INFO @ Sat, 11 Dec 2021 16:19:25: 25000000 INFO @ Sat, 11 Dec 2021 16:19:31: 20000000 INFO @ Sat, 11 Dec 2021 16:19:33: 26000000 INFO @ Sat, 11 Dec 2021 16:19:34: 26000000 INFO @ Sat, 11 Dec 2021 16:19:41: 21000000 INFO @ Sat, 11 Dec 2021 16:19:42: 27000000 INFO @ Sat, 11 Dec 2021 16:19:44: 27000000 INFO @ Sat, 11 Dec 2021 16:19:50: 28000000 INFO @ Sat, 11 Dec 2021 16:19:50: 22000000 INFO @ Sat, 11 Dec 2021 16:19:53: 28000000 INFO @ Sat, 11 Dec 2021 16:19:58: 29000000 INFO @ Sat, 11 Dec 2021 16:20:00: 23000000 INFO @ Sat, 11 Dec 2021 16:20:03: 29000000 INFO @ Sat, 11 Dec 2021 16:20:07: 30000000 INFO @ Sat, 11 Dec 2021 16:20:09: 24000000 INFO @ Sat, 11 Dec 2021 16:20:13: 30000000 INFO @ Sat, 11 Dec 2021 16:20:15: 31000000 INFO @ Sat, 11 Dec 2021 16:20:18: 25000000 INFO @ Sat, 11 Dec 2021 16:20:24: 32000000 INFO @ Sat, 11 Dec 2021 16:20:24: 31000000 INFO @ Sat, 11 Dec 2021 16:20:28: 26000000 INFO @ Sat, 11 Dec 2021 16:20:33: 33000000 INFO @ Sat, 11 Dec 2021 16:20:34: 32000000 INFO @ Sat, 11 Dec 2021 16:20:37: 27000000 INFO @ Sat, 11 Dec 2021 16:20:41: 34000000 INFO @ Sat, 11 Dec 2021 16:20:43: 33000000 INFO @ Sat, 11 Dec 2021 16:20:47: 28000000 INFO @ Sat, 11 Dec 2021 16:20:50: 35000000 INFO @ Sat, 11 Dec 2021 16:20:53: 34000000 INFO @ Sat, 11 Dec 2021 16:20:56: 29000000 INFO @ Sat, 11 Dec 2021 16:20:58: 36000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 16:21:02: 35000000 INFO @ Sat, 11 Dec 2021 16:21:06: 30000000 INFO @ Sat, 11 Dec 2021 16:21:06: 37000000 INFO @ Sat, 11 Dec 2021 16:21:12: 36000000 INFO @ Sat, 11 Dec 2021 16:21:14: 38000000 INFO @ Sat, 11 Dec 2021 16:21:15: 31000000 INFO @ Sat, 11 Dec 2021 16:21:21: 37000000 INFO @ Sat, 11 Dec 2021 16:21:23: 39000000 INFO @ Sat, 11 Dec 2021 16:21:24: 32000000 INFO @ Sat, 11 Dec 2021 16:21:31: 40000000 INFO @ Sat, 11 Dec 2021 16:21:31: 38000000 INFO @ Sat, 11 Dec 2021 16:21:34: 33000000 INFO @ Sat, 11 Dec 2021 16:21:39: 41000000 INFO @ Sat, 11 Dec 2021 16:21:41: 39000000 INFO @ Sat, 11 Dec 2021 16:21:43: 34000000 INFO @ Sat, 11 Dec 2021 16:21:47: 42000000 INFO @ Sat, 11 Dec 2021 16:21:51: 40000000 INFO @ Sat, 11 Dec 2021 16:21:53: 35000000 INFO @ Sat, 11 Dec 2021 16:21:56: 43000000 INFO @ Sat, 11 Dec 2021 16:22:00: 41000000 INFO @ Sat, 11 Dec 2021 16:22:02: 36000000 INFO @ Sat, 11 Dec 2021 16:22:04: 44000000 INFO @ Sat, 11 Dec 2021 16:22:10: 42000000 INFO @ Sat, 11 Dec 2021 16:22:12: 37000000 INFO @ Sat, 11 Dec 2021 16:22:12: 45000000 INFO @ Sat, 11 Dec 2021 16:22:19: 43000000 INFO @ Sat, 11 Dec 2021 16:22:21: 46000000 INFO @ Sat, 11 Dec 2021 16:22:21: 38000000 INFO @ Sat, 11 Dec 2021 16:22:29: 44000000 INFO @ Sat, 11 Dec 2021 16:22:29: 47000000 INFO @ Sat, 11 Dec 2021 16:22:30: 39000000 INFO @ Sat, 11 Dec 2021 16:22:37: 48000000 INFO @ Sat, 11 Dec 2021 16:22:39: 45000000 INFO @ Sat, 11 Dec 2021 16:22:39: 40000000 INFO @ Sat, 11 Dec 2021 16:22:45: 49000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 16:22:48: 46000000 INFO @ Sat, 11 Dec 2021 16:22:49: 41000000 INFO @ Sat, 11 Dec 2021 16:22:54: 50000000 INFO @ Sat, 11 Dec 2021 16:22:57: 47000000 INFO @ Sat, 11 Dec 2021 16:22:58: 42000000 INFO @ Sat, 11 Dec 2021 16:23:02: 51000000 INFO @ Sat, 11 Dec 2021 16:23:07: 48000000 INFO @ Sat, 11 Dec 2021 16:23:07: 43000000 INFO @ Sat, 11 Dec 2021 16:23:10: 52000000 INFO @ Sat, 11 Dec 2021 16:23:16: 49000000 INFO @ Sat, 11 Dec 2021 16:23:17: 44000000 INFO @ Sat, 11 Dec 2021 16:23:18: 53000000 INFO @ Sat, 11 Dec 2021 16:23:26: 50000000 INFO @ Sat, 11 Dec 2021 16:23:27: 45000000 INFO @ Sat, 11 Dec 2021 16:23:27: 54000000 INFO @ Sat, 11 Dec 2021 16:23:35: 55000000 INFO @ Sat, 11 Dec 2021 16:23:35: 51000000 INFO @ Sat, 11 Dec 2021 16:23:36: 46000000 INFO @ Sat, 11 Dec 2021 16:23:43: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 16:23:43: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 16:23:43: #1 total tags in treatment: 21535146 INFO @ Sat, 11 Dec 2021 16:23:43: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:23:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:23:43: #1 tags after filtering in treatment: 16002891 INFO @ Sat, 11 Dec 2021 16:23:43: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 11 Dec 2021 16:23:43: #1 finished! INFO @ Sat, 11 Dec 2021 16:23:43: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:23:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:23:44: #2 number of paired peaks: 105 WARNING @ Sat, 11 Dec 2021 16:23:44: Fewer paired peaks (105) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 105 pairs to build model! INFO @ Sat, 11 Dec 2021 16:23:44: start model_add_line... INFO @ Sat, 11 Dec 2021 16:23:44: 52000000 INFO @ Sat, 11 Dec 2021 16:23:44: start X-correlation... INFO @ Sat, 11 Dec 2021 16:23:44: end of X-cor INFO @ Sat, 11 Dec 2021 16:23:44: #2 finished! INFO @ Sat, 11 Dec 2021 16:23:44: #2 predicted fragment length is 72 bps INFO @ Sat, 11 Dec 2021 16:23:44: #2 alternative fragment length(s) may be 3,13,72,95,557,564,576 bps INFO @ Sat, 11 Dec 2021 16:23:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.10_model.r WARNING @ Sat, 11 Dec 2021 16:23:44: #2 Since the d (72) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 16:23:44: #2 You may need to consider one of the other alternative d(s): 3,13,72,95,557,564,576 WARNING @ Sat, 11 Dec 2021 16:23:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 16:23:44: #3 Call peaks... INFO @ Sat, 11 Dec 2021 16:23:44: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 16:23:46: 47000000 INFO @ Sat, 11 Dec 2021 16:23:54: 53000000 INFO @ Sat, 11 Dec 2021 16:23:55: 48000000 INFO @ Sat, 11 Dec 2021 16:24:03: 54000000 INFO @ Sat, 11 Dec 2021 16:24:05: 49000000 INFO @ Sat, 11 Dec 2021 16:24:13: 55000000 INFO @ Sat, 11 Dec 2021 16:24:14: 50000000 INFO @ Sat, 11 Dec 2021 16:24:14: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 16:24:22: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 16:24:22: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 16:24:22: #1 total tags in treatment: 21535146 INFO @ Sat, 11 Dec 2021 16:24:22: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:24:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:24:22: #1 tags after filtering in treatment: 16002891 INFO @ Sat, 11 Dec 2021 16:24:22: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 11 Dec 2021 16:24:22: #1 finished! INFO @ Sat, 11 Dec 2021 16:24:22: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:24:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:24:23: 51000000 INFO @ Sat, 11 Dec 2021 16:24:23: #2 number of paired peaks: 105 WARNING @ Sat, 11 Dec 2021 16:24:23: Fewer paired peaks (105) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 105 pairs to build model! INFO @ Sat, 11 Dec 2021 16:24:23: start model_add_line... INFO @ Sat, 11 Dec 2021 16:24:23: start X-correlation... INFO @ Sat, 11 Dec 2021 16:24:23: end of X-cor INFO @ Sat, 11 Dec 2021 16:24:23: #2 finished! INFO @ Sat, 11 Dec 2021 16:24:23: #2 predicted fragment length is 72 bps INFO @ Sat, 11 Dec 2021 16:24:23: #2 alternative fragment length(s) may be 3,13,72,95,557,564,576 bps INFO @ Sat, 11 Dec 2021 16:24:23: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.05_model.r WARNING @ Sat, 11 Dec 2021 16:24:23: #2 Since the d (72) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 16:24:23: #2 You may need to consider one of the other alternative d(s): 3,13,72,95,557,564,576 WARNING @ Sat, 11 Dec 2021 16:24:23: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 16:24:23: #3 Call peaks... INFO @ Sat, 11 Dec 2021 16:24:23: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 16:24:32: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.10_peaks.xls INFO @ Sat, 11 Dec 2021 16:24:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 16:24:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.10_summits.bed INFO @ Sat, 11 Dec 2021 16:24:32: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (3513 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 16:24:32: 52000000 INFO @ Sat, 11 Dec 2021 16:24:42: 53000000 INFO @ Sat, 11 Dec 2021 16:24:51: 54000000 INFO @ Sat, 11 Dec 2021 16:24:53: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 16:25:00: 55000000 INFO @ Sat, 11 Dec 2021 16:25:08: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 16:25:08: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 16:25:08: #1 total tags in treatment: 21535146 INFO @ Sat, 11 Dec 2021 16:25:08: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:25:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:25:09: #1 tags after filtering in treatment: 16002891 INFO @ Sat, 11 Dec 2021 16:25:09: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 11 Dec 2021 16:25:09: #1 finished! INFO @ Sat, 11 Dec 2021 16:25:09: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:25:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:25:10: #2 number of paired peaks: 105 WARNING @ Sat, 11 Dec 2021 16:25:10: Fewer paired peaks (105) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 105 pairs to build model! INFO @ Sat, 11 Dec 2021 16:25:10: start model_add_line... INFO @ Sat, 11 Dec 2021 16:25:10: start X-correlation... INFO @ Sat, 11 Dec 2021 16:25:10: end of X-cor INFO @ Sat, 11 Dec 2021 16:25:10: #2 finished! INFO @ Sat, 11 Dec 2021 16:25:10: #2 predicted fragment length is 72 bps INFO @ Sat, 11 Dec 2021 16:25:10: #2 alternative fragment length(s) may be 3,13,72,95,557,564,576 bps INFO @ Sat, 11 Dec 2021 16:25:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.20_model.r WARNING @ Sat, 11 Dec 2021 16:25:10: #2 Since the d (72) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 16:25:10: #2 You may need to consider one of the other alternative d(s): 3,13,72,95,557,564,576 WARNING @ Sat, 11 Dec 2021 16:25:10: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 16:25:10: #3 Call peaks... INFO @ Sat, 11 Dec 2021 16:25:10: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 16:25:11: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.05_peaks.xls INFO @ Sat, 11 Dec 2021 16:25:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 16:25:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.05_summits.bed INFO @ Sat, 11 Dec 2021 16:25:11: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (8562 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 16:25:39: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 16:25:57: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.20_peaks.xls INFO @ Sat, 11 Dec 2021 16:25:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 16:25:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9103828/SRX9103828.20_summits.bed INFO @ Sat, 11 Dec 2021 16:25:57: Done! pass1 - making usageList (12 chroms): 1 millis pass2 - checking and writing primary data (499 records, 4 fields): 2 millis CompletedMACS2peakCalling