Job ID = 14172102 SRX = SRX9103810 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 25172111 spots for SRR12621010/SRR12621010.sra Written 25172111 spots for SRR12621010/SRR12621010.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172684 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:39:03 25172111 reads; of these: 25172111 (100.00%) were paired; of these: 5939071 (23.59%) aligned concordantly 0 times 13654916 (54.25%) aligned concordantly exactly 1 time 5578124 (22.16%) aligned concordantly >1 times ---- 5939071 pairs aligned concordantly 0 times; of these: 2103380 (35.42%) aligned discordantly 1 time ---- 3835691 pairs aligned 0 times concordantly or discordantly; of these: 7671382 mates make up the pairs; of these: 5707139 (74.40%) aligned 0 times 775001 (10.10%) aligned exactly 1 time 1189242 (15.50%) aligned >1 times 88.66% overall alignment rate Time searching: 00:39:04 Overall time: 00:39:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3916742 / 21039799 = 0.1862 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:12:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:12:11: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:12:11: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:12:21: 1000000 INFO @ Sat, 11 Dec 2021 15:12:31: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:12:40: 3000000 INFO @ Sat, 11 Dec 2021 15:12:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:12:41: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:12:41: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:12:49: 4000000 INFO @ Sat, 11 Dec 2021 15:12:52: 1000000 INFO @ Sat, 11 Dec 2021 15:12:58: 5000000 INFO @ Sat, 11 Dec 2021 15:13:02: 2000000 INFO @ Sat, 11 Dec 2021 15:13:06: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:13:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:13:11: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:13:11: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:13:12: 3000000 INFO @ Sat, 11 Dec 2021 15:13:14: 7000000 INFO @ Sat, 11 Dec 2021 15:13:22: 1000000 INFO @ Sat, 11 Dec 2021 15:13:23: 4000000 INFO @ Sat, 11 Dec 2021 15:13:23: 8000000 INFO @ Sat, 11 Dec 2021 15:13:31: 9000000 INFO @ Sat, 11 Dec 2021 15:13:32: 2000000 INFO @ Sat, 11 Dec 2021 15:13:34: 5000000 INFO @ Sat, 11 Dec 2021 15:13:40: 10000000 INFO @ Sat, 11 Dec 2021 15:13:43: 3000000 INFO @ Sat, 11 Dec 2021 15:13:45: 6000000 INFO @ Sat, 11 Dec 2021 15:13:48: 11000000 INFO @ Sat, 11 Dec 2021 15:13:53: 4000000 INFO @ Sat, 11 Dec 2021 15:13:56: 7000000 INFO @ Sat, 11 Dec 2021 15:13:57: 12000000 INFO @ Sat, 11 Dec 2021 15:14:05: 5000000 INFO @ Sat, 11 Dec 2021 15:14:05: 13000000 INFO @ Sat, 11 Dec 2021 15:14:07: 8000000 INFO @ Sat, 11 Dec 2021 15:14:13: 14000000 INFO @ Sat, 11 Dec 2021 15:14:16: 6000000 INFO @ Sat, 11 Dec 2021 15:14:19: 9000000 INFO @ Sat, 11 Dec 2021 15:14:22: 15000000 INFO @ Sat, 11 Dec 2021 15:14:28: 7000000 INFO @ Sat, 11 Dec 2021 15:14:30: 16000000 INFO @ Sat, 11 Dec 2021 15:14:30: 10000000 INFO @ Sat, 11 Dec 2021 15:14:38: 17000000 INFO @ Sat, 11 Dec 2021 15:14:39: 8000000 INFO @ Sat, 11 Dec 2021 15:14:42: 11000000 INFO @ Sat, 11 Dec 2021 15:14:47: 18000000 INFO @ Sat, 11 Dec 2021 15:14:50: 9000000 INFO @ Sat, 11 Dec 2021 15:14:54: 12000000 INFO @ Sat, 11 Dec 2021 15:14:55: 19000000 INFO @ Sat, 11 Dec 2021 15:15:02: 10000000 INFO @ Sat, 11 Dec 2021 15:15:05: 20000000 INFO @ Sat, 11 Dec 2021 15:15:06: 13000000 INFO @ Sat, 11 Dec 2021 15:15:13: 21000000 INFO @ Sat, 11 Dec 2021 15:15:13: 11000000 INFO @ Sat, 11 Dec 2021 15:15:17: 14000000 INFO @ Sat, 11 Dec 2021 15:15:22: 22000000 INFO @ Sat, 11 Dec 2021 15:15:24: 12000000 INFO @ Sat, 11 Dec 2021 15:15:29: 15000000 INFO @ Sat, 11 Dec 2021 15:15:31: 23000000 INFO @ Sat, 11 Dec 2021 15:15:36: 13000000 INFO @ Sat, 11 Dec 2021 15:15:41: 16000000 INFO @ Sat, 11 Dec 2021 15:15:41: 24000000 INFO @ Sat, 11 Dec 2021 15:15:47: 14000000 INFO @ Sat, 11 Dec 2021 15:15:51: 25000000 INFO @ Sat, 11 Dec 2021 15:15:53: 17000000 INFO @ Sat, 11 Dec 2021 15:15:59: 15000000 INFO @ Sat, 11 Dec 2021 15:16:01: 26000000 INFO @ Sat, 11 Dec 2021 15:16:04: 18000000 INFO @ Sat, 11 Dec 2021 15:16:10: 27000000 INFO @ Sat, 11 Dec 2021 15:16:11: 16000000 INFO @ Sat, 11 Dec 2021 15:16:16: 19000000 INFO @ Sat, 11 Dec 2021 15:16:18: 28000000 INFO @ Sat, 11 Dec 2021 15:16:22: 17000000 INFO @ Sat, 11 Dec 2021 15:16:27: 29000000 INFO @ Sat, 11 Dec 2021 15:16:28: 20000000 INFO @ Sat, 11 Dec 2021 15:16:34: 18000000 INFO @ Sat, 11 Dec 2021 15:16:36: 30000000 INFO @ Sat, 11 Dec 2021 15:16:40: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 15:16:45: 19000000 INFO @ Sat, 11 Dec 2021 15:16:46: 31000000 INFO @ Sat, 11 Dec 2021 15:16:51: 22000000 INFO @ Sat, 11 Dec 2021 15:16:55: 32000000 INFO @ Sat, 11 Dec 2021 15:16:56: 20000000 INFO @ Sat, 11 Dec 2021 15:17:03: 23000000 INFO @ Sat, 11 Dec 2021 15:17:04: 33000000 INFO @ Sat, 11 Dec 2021 15:17:08: 21000000 INFO @ Sat, 11 Dec 2021 15:17:13: 34000000 INFO @ Sat, 11 Dec 2021 15:17:15: 24000000 INFO @ Sat, 11 Dec 2021 15:17:20: 22000000 INFO @ Sat, 11 Dec 2021 15:17:22: 35000000 INFO @ Sat, 11 Dec 2021 15:17:28: 25000000 INFO @ Sat, 11 Dec 2021 15:17:30: 36000000 INFO @ Sat, 11 Dec 2021 15:17:31: 23000000 INFO @ Sat, 11 Dec 2021 15:17:37: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:17:37: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:17:37: #1 total tags in treatment: 15484007 INFO @ Sat, 11 Dec 2021 15:17:37: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:17:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:17:37: #1 tags after filtering in treatment: 12862938 INFO @ Sat, 11 Dec 2021 15:17:37: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 11 Dec 2021 15:17:37: #1 finished! INFO @ Sat, 11 Dec 2021 15:17:37: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:17:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:17:38: #2 number of paired peaks: 66 WARNING @ Sat, 11 Dec 2021 15:17:38: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 15:17:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:17:41: 26000000 INFO @ Sat, 11 Dec 2021 15:17:43: 24000000 INFO @ Sat, 11 Dec 2021 15:17:54: 27000000 INFO @ Sat, 11 Dec 2021 15:17:54: 25000000 INFO @ Sat, 11 Dec 2021 15:18:06: 26000000 INFO @ Sat, 11 Dec 2021 15:18:07: 28000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 15:18:17: 27000000 INFO @ Sat, 11 Dec 2021 15:18:18: 29000000 INFO @ Sat, 11 Dec 2021 15:18:29: 30000000 INFO @ Sat, 11 Dec 2021 15:18:29: 28000000 INFO @ Sat, 11 Dec 2021 15:18:40: 31000000 INFO @ Sat, 11 Dec 2021 15:18:40: 29000000 INFO @ Sat, 11 Dec 2021 15:18:52: 30000000 INFO @ Sat, 11 Dec 2021 15:18:52: 32000000 INFO @ Sat, 11 Dec 2021 15:19:03: 31000000 INFO @ Sat, 11 Dec 2021 15:19:05: 33000000 INFO @ Sat, 11 Dec 2021 15:19:14: 32000000 INFO @ Sat, 11 Dec 2021 15:19:17: 34000000 INFO @ Sat, 11 Dec 2021 15:19:26: 33000000 INFO @ Sat, 11 Dec 2021 15:19:29: 35000000 INFO @ Sat, 11 Dec 2021 15:19:39: 34000000 INFO @ Sat, 11 Dec 2021 15:19:42: 36000000 INFO @ Sat, 11 Dec 2021 15:19:51: 35000000 INFO @ Sat, 11 Dec 2021 15:19:52: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:19:52: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:19:52: #1 total tags in treatment: 15484007 INFO @ Sat, 11 Dec 2021 15:19:52: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:19:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:19:52: #1 tags after filtering in treatment: 12862938 INFO @ Sat, 11 Dec 2021 15:19:52: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 11 Dec 2021 15:19:52: #1 finished! INFO @ Sat, 11 Dec 2021 15:19:52: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:19:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:19:54: #2 number of paired peaks: 66 WARNING @ Sat, 11 Dec 2021 15:19:54: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 15:19:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:20:04: 36000000 INFO @ Sat, 11 Dec 2021 15:20:15: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:20:15: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:20:15: #1 total tags in treatment: 15484007 INFO @ Sat, 11 Dec 2021 15:20:15: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:20:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:20:15: #1 tags after filtering in treatment: 12862938 INFO @ Sat, 11 Dec 2021 15:20:15: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 11 Dec 2021 15:20:15: #1 finished! INFO @ Sat, 11 Dec 2021 15:20:15: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:20:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:20:16: #2 number of paired peaks: 66 WARNING @ Sat, 11 Dec 2021 15:20:16: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 15:20:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9103810/SRX9103810.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling