Job ID = 14167189 SRX = SRX9090280 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 39238948 spots for SRR12606613/SRR12606613.sra Written 39238948 spots for SRR12606613/SRR12606613.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167748 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:35 39238948 reads; of these: 39238948 (100.00%) were unpaired; of these: 1568466 (4.00%) aligned 0 times 26809116 (68.32%) aligned exactly 1 time 10861366 (27.68%) aligned >1 times 96.00% overall alignment rate Time searching: 00:11:36 Overall time: 00:11:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 17252962 / 37670482 = 0.4580 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:10:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:10:32: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:10:32: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:10:37: 1000000 INFO @ Fri, 10 Dec 2021 11:10:42: 2000000 INFO @ Fri, 10 Dec 2021 11:10:47: 3000000 INFO @ Fri, 10 Dec 2021 11:10:52: 4000000 INFO @ Fri, 10 Dec 2021 11:10:57: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:11:02: 6000000 INFO @ Fri, 10 Dec 2021 11:11:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:11:02: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:11:02: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:11:08: 7000000 INFO @ Fri, 10 Dec 2021 11:11:08: 1000000 INFO @ Fri, 10 Dec 2021 11:11:14: 8000000 INFO @ Fri, 10 Dec 2021 11:11:15: 2000000 INFO @ Fri, 10 Dec 2021 11:11:20: 9000000 INFO @ Fri, 10 Dec 2021 11:11:21: 3000000 INFO @ Fri, 10 Dec 2021 11:11:26: 10000000 INFO @ Fri, 10 Dec 2021 11:11:28: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:11:32: 11000000 INFO @ Fri, 10 Dec 2021 11:11:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:11:32: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:11:32: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:11:34: 5000000 INFO @ Fri, 10 Dec 2021 11:11:38: 12000000 INFO @ Fri, 10 Dec 2021 11:11:38: 1000000 INFO @ Fri, 10 Dec 2021 11:11:40: 6000000 INFO @ Fri, 10 Dec 2021 11:11:43: 13000000 INFO @ Fri, 10 Dec 2021 11:11:45: 2000000 INFO @ Fri, 10 Dec 2021 11:11:47: 7000000 INFO @ Fri, 10 Dec 2021 11:11:49: 14000000 INFO @ Fri, 10 Dec 2021 11:11:52: 3000000 INFO @ Fri, 10 Dec 2021 11:11:53: 8000000 INFO @ Fri, 10 Dec 2021 11:11:55: 15000000 INFO @ Fri, 10 Dec 2021 11:11:58: 4000000 INFO @ Fri, 10 Dec 2021 11:11:59: 9000000 INFO @ Fri, 10 Dec 2021 11:12:01: 16000000 INFO @ Fri, 10 Dec 2021 11:12:04: 5000000 INFO @ Fri, 10 Dec 2021 11:12:06: 10000000 INFO @ Fri, 10 Dec 2021 11:12:07: 17000000 INFO @ Fri, 10 Dec 2021 11:12:11: 6000000 INFO @ Fri, 10 Dec 2021 11:12:12: 11000000 INFO @ Fri, 10 Dec 2021 11:12:13: 18000000 INFO @ Fri, 10 Dec 2021 11:12:17: 7000000 INFO @ Fri, 10 Dec 2021 11:12:19: 12000000 INFO @ Fri, 10 Dec 2021 11:12:19: 19000000 INFO @ Fri, 10 Dec 2021 11:12:23: 8000000 INFO @ Fri, 10 Dec 2021 11:12:25: 20000000 INFO @ Fri, 10 Dec 2021 11:12:25: 13000000 INFO @ Fri, 10 Dec 2021 11:12:27: #1 tag size is determined as 49 bps INFO @ Fri, 10 Dec 2021 11:12:27: #1 tag size = 49 INFO @ Fri, 10 Dec 2021 11:12:27: #1 total tags in treatment: 20417520 INFO @ Fri, 10 Dec 2021 11:12:27: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:12:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:12:28: #1 tags after filtering in treatment: 20417520 INFO @ Fri, 10 Dec 2021 11:12:28: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:12:28: #1 finished! INFO @ Fri, 10 Dec 2021 11:12:28: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:12:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:12:29: #2 number of paired peaks: 4083 INFO @ Fri, 10 Dec 2021 11:12:29: start model_add_line... INFO @ Fri, 10 Dec 2021 11:12:30: start X-correlation... INFO @ Fri, 10 Dec 2021 11:12:30: end of X-cor INFO @ Fri, 10 Dec 2021 11:12:30: #2 finished! INFO @ Fri, 10 Dec 2021 11:12:30: #2 predicted fragment length is 111 bps INFO @ Fri, 10 Dec 2021 11:12:30: #2 alternative fragment length(s) may be 111 bps INFO @ Fri, 10 Dec 2021 11:12:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.05_model.r INFO @ Fri, 10 Dec 2021 11:12:30: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:12:30: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:12:30: 9000000 INFO @ Fri, 10 Dec 2021 11:12:32: 14000000 INFO @ Fri, 10 Dec 2021 11:12:36: 10000000 INFO @ Fri, 10 Dec 2021 11:12:38: 15000000 INFO @ Fri, 10 Dec 2021 11:12:43: 11000000 INFO @ Fri, 10 Dec 2021 11:12:45: 16000000 INFO @ Fri, 10 Dec 2021 11:12:49: 12000000 INFO @ Fri, 10 Dec 2021 11:12:51: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 11:12:55: 13000000 INFO @ Fri, 10 Dec 2021 11:12:57: 18000000 INFO @ Fri, 10 Dec 2021 11:13:01: 14000000 INFO @ Fri, 10 Dec 2021 11:13:04: 19000000 INFO @ Fri, 10 Dec 2021 11:13:08: 15000000 INFO @ Fri, 10 Dec 2021 11:13:10: 20000000 INFO @ Fri, 10 Dec 2021 11:13:11: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:13:13: #1 tag size is determined as 49 bps INFO @ Fri, 10 Dec 2021 11:13:13: #1 tag size = 49 INFO @ Fri, 10 Dec 2021 11:13:13: #1 total tags in treatment: 20417520 INFO @ Fri, 10 Dec 2021 11:13:13: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:13:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:13:13: #1 tags after filtering in treatment: 20417520 INFO @ Fri, 10 Dec 2021 11:13:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:13:13: #1 finished! INFO @ Fri, 10 Dec 2021 11:13:13: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:13:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:13:14: 16000000 INFO @ Fri, 10 Dec 2021 11:13:15: #2 number of paired peaks: 4083 INFO @ Fri, 10 Dec 2021 11:13:15: start model_add_line... INFO @ Fri, 10 Dec 2021 11:13:15: start X-correlation... INFO @ Fri, 10 Dec 2021 11:13:15: end of X-cor INFO @ Fri, 10 Dec 2021 11:13:15: #2 finished! INFO @ Fri, 10 Dec 2021 11:13:15: #2 predicted fragment length is 111 bps INFO @ Fri, 10 Dec 2021 11:13:15: #2 alternative fragment length(s) may be 111 bps INFO @ Fri, 10 Dec 2021 11:13:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.10_model.r INFO @ Fri, 10 Dec 2021 11:13:15: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:13:15: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:13:20: 17000000 INFO @ Fri, 10 Dec 2021 11:13:26: 18000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 11:13:31: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.05_peaks.xls INFO @ Fri, 10 Dec 2021 11:13:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:13:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.05_summits.bed INFO @ Fri, 10 Dec 2021 11:13:31: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (10802 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:13:32: 19000000 INFO @ Fri, 10 Dec 2021 11:13:38: 20000000 INFO @ Fri, 10 Dec 2021 11:13:41: #1 tag size is determined as 49 bps INFO @ Fri, 10 Dec 2021 11:13:41: #1 tag size = 49 INFO @ Fri, 10 Dec 2021 11:13:41: #1 total tags in treatment: 20417520 INFO @ Fri, 10 Dec 2021 11:13:41: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:13:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:13:41: #1 tags after filtering in treatment: 20417520 INFO @ Fri, 10 Dec 2021 11:13:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:13:41: #1 finished! INFO @ Fri, 10 Dec 2021 11:13:41: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:13:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:13:43: #2 number of paired peaks: 4083 INFO @ Fri, 10 Dec 2021 11:13:43: start model_add_line... INFO @ Fri, 10 Dec 2021 11:13:43: start X-correlation... INFO @ Fri, 10 Dec 2021 11:13:43: end of X-cor INFO @ Fri, 10 Dec 2021 11:13:43: #2 finished! INFO @ Fri, 10 Dec 2021 11:13:43: #2 predicted fragment length is 111 bps INFO @ Fri, 10 Dec 2021 11:13:43: #2 alternative fragment length(s) may be 111 bps INFO @ Fri, 10 Dec 2021 11:13:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.20_model.r INFO @ Fri, 10 Dec 2021 11:13:43: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:13:43: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:13:56: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:14:16: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.10_peaks.xls INFO @ Fri, 10 Dec 2021 11:14:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:14:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.10_summits.bed INFO @ Fri, 10 Dec 2021 11:14:16: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (8891 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:14:25: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:14:44: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.20_peaks.xls INFO @ Fri, 10 Dec 2021 11:14:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:14:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9090280/SRX9090280.20_summits.bed INFO @ Fri, 10 Dec 2021 11:14:44: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (6385 records, 4 fields): 7 millis CompletedMACS2peakCalling