Job ID = 14171544 SRX = SRX9008800 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 21451417 spots for SRR12518272/SRR12518272.sra Written 21451417 spots for SRR12518272/SRR12518272.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172007 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:50 21451417 reads; of these: 21451417 (100.00%) were unpaired; of these: 4724631 (22.02%) aligned 0 times 14164712 (66.03%) aligned exactly 1 time 2562074 (11.94%) aligned >1 times 77.98% overall alignment rate Time searching: 00:04:50 Overall time: 00:04:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2993683 / 16726786 = 0.1790 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:06:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:06:53: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:06:53: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:06:57: 1000000 INFO @ Sat, 11 Dec 2021 12:07:02: 2000000 INFO @ Sat, 11 Dec 2021 12:07:07: 3000000 INFO @ Sat, 11 Dec 2021 12:07:11: 4000000 INFO @ Sat, 11 Dec 2021 12:07:16: 5000000 INFO @ Sat, 11 Dec 2021 12:07:20: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:07:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:07:23: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:07:23: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:07:25: 7000000 INFO @ Sat, 11 Dec 2021 12:07:28: 1000000 INFO @ Sat, 11 Dec 2021 12:07:29: 8000000 INFO @ Sat, 11 Dec 2021 12:07:33: 2000000 INFO @ Sat, 11 Dec 2021 12:07:34: 9000000 INFO @ Sat, 11 Dec 2021 12:07:38: 3000000 INFO @ Sat, 11 Dec 2021 12:07:39: 10000000 INFO @ Sat, 11 Dec 2021 12:07:42: 4000000 INFO @ Sat, 11 Dec 2021 12:07:43: 11000000 INFO @ Sat, 11 Dec 2021 12:07:47: 5000000 INFO @ Sat, 11 Dec 2021 12:07:48: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:07:52: 6000000 INFO @ Sat, 11 Dec 2021 12:07:52: 13000000 INFO @ Sat, 11 Dec 2021 12:07:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:07:53: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:07:53: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:07:56: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:07:56: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:07:56: #1 total tags in treatment: 13733103 INFO @ Sat, 11 Dec 2021 12:07:56: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:07:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:07:56: #1 tags after filtering in treatment: 13733103 INFO @ Sat, 11 Dec 2021 12:07:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:07:56: #1 finished! INFO @ Sat, 11 Dec 2021 12:07:56: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:07:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:07:57: 7000000 INFO @ Sat, 11 Dec 2021 12:07:57: #2 number of paired peaks: 50 WARNING @ Sat, 11 Dec 2021 12:07:57: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:07:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:07:57: 1000000 INFO @ Sat, 11 Dec 2021 12:08:02: 8000000 INFO @ Sat, 11 Dec 2021 12:08:02: 2000000 INFO @ Sat, 11 Dec 2021 12:08:07: 9000000 INFO @ Sat, 11 Dec 2021 12:08:07: 3000000 INFO @ Sat, 11 Dec 2021 12:08:11: 4000000 INFO @ Sat, 11 Dec 2021 12:08:11: 10000000 INFO @ Sat, 11 Dec 2021 12:08:16: 5000000 INFO @ Sat, 11 Dec 2021 12:08:16: 11000000 INFO @ Sat, 11 Dec 2021 12:08:20: 6000000 INFO @ Sat, 11 Dec 2021 12:08:21: 12000000 INFO @ Sat, 11 Dec 2021 12:08:25: 7000000 INFO @ Sat, 11 Dec 2021 12:08:26: 13000000 INFO @ Sat, 11 Dec 2021 12:08:30: 8000000 INFO @ Sat, 11 Dec 2021 12:08:30: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:08:30: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:08:30: #1 total tags in treatment: 13733103 INFO @ Sat, 11 Dec 2021 12:08:30: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:08:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:08:30: #1 tags after filtering in treatment: 13733103 INFO @ Sat, 11 Dec 2021 12:08:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:08:30: #1 finished! INFO @ Sat, 11 Dec 2021 12:08:30: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:08:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:08:31: #2 number of paired peaks: 50 WARNING @ Sat, 11 Dec 2021 12:08:31: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:08:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:08:34: 9000000 INFO @ Sat, 11 Dec 2021 12:08:39: 10000000 INFO @ Sat, 11 Dec 2021 12:08:43: 11000000 INFO @ Sat, 11 Dec 2021 12:08:48: 12000000 INFO @ Sat, 11 Dec 2021 12:08:52: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 12:08:56: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:08:56: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:08:56: #1 total tags in treatment: 13733103 INFO @ Sat, 11 Dec 2021 12:08:56: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:08:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:08:56: #1 tags after filtering in treatment: 13733103 INFO @ Sat, 11 Dec 2021 12:08:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:08:56: #1 finished! INFO @ Sat, 11 Dec 2021 12:08:56: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:08:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:08:57: #2 number of paired peaks: 50 WARNING @ Sat, 11 Dec 2021 12:08:57: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:08:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。